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Specific activities of DvLysin and BoYkfC mutants
| Substrate | Sp act (nmol/min/mg) of WT or mutant forma: |
|||||
|---|---|---|---|---|---|---|
| DvLysin |
BoYkfC |
|||||
| WT | C333A | A211Y | WT | Y80A | Y80A D221N | |
| Peptidoglycan | >35 | NA | NA | NA | NA | NA |
| TCT | 5500 | 0.7 | 90 | NA | NA | 0.6 |
| MurNAc-tetrapeptide | 5400 | 0.7 | 60 | NA | NA | 1.1 |
| Lactoyl-pentapeptide | 2300 | 10 | 1100 | 1.5 | NA | NA |
| Tetrapeptide | 6100 | 30 | 4900 | 25,800 | 3500 | 3300 |
NA, no activity detected with up to 20 µg of protein under the assay conditions used.
