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. 2015 Oct 9;8:519. doi: 10.1186/s13071-015-1134-5

Erratum to: Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

B L Webster 1,2,, M Rabone 1, T Pennance 1,3, A M Emery 1, F Allan 1, A Gouvras 1, S Knopp 1,4,5, A Garba 6, A A Hamidou 6, K A Mohammed 7, S M Ame 7, D Rollinson 1, J P Webster 2,3
PMCID: PMC4600265  PMID: 26453014

Unfortunately, the original version of this article [1], contained a mistake. In Table 1, the primers for Sh6 and Sh9 were included incorrectly. Instead of GGGATGTATGCAGACTTG TTGTTTGGCTGCAGTAAC and GCTGAGCTTGAGATTG CTTCTGTCCCATCGATACC they should have been Sh6 Forward Primer GGTGGATTACGCAATAG, Sh6 Reverse Primer TTTAATCAACCGGGTGTC and Sh9 Forward Primer GGGATGTATGCAGACTTG, Sh9 Reverse Primer TTGTTTGGCTGCAGTAAC respectively.

Table 1.

Details of the 18 selected microsatellite loci and the characteristics of the two multiplex microsatellite PCR assays. Loci Sh1-15 are from Travis et al., 2013 and Loci C102, C111 and C131 are from Gower et al., 2011. For Niger Ho = 0.596, He = 0.609, for Pemba Ho = 0.599, He = 0.638. The overall Ho = 0.597, He = 0.623

Panel 1 Marker Forward Primer 5'- 3' Reverse Primer 5'- 3' Dye Size Range (bp) Repeat A Niger Zanzibar
H o H e H o H e
Panel 1 C102 TGTCTCTGTGAATGACCGAAT TTAGATGAATAATAATGTTGAAACCAC VIC 184-199 ATT 6 0.42 0.37 0.02 0.02
Sh1 GCATCCAATTTCGTACAC CCACATTAGGCCAACAAG VIC 245-284 AAT 13 0.76 0.72 0.84 0.80
Sh14 GTCCTCCTTCCCTCTTTG CACATTCGTCCTAGATATCG NED 184-240 ACTC 15 0.94 0.85 0.86 0.88
C131 CTTGTCATTTGGGCATTGTG CATGGTGAGGTTCAAACGTG NED 253-265 AAT 4 0.00 0.00 0.00 0.00
Sh6 GGTGGATTACGCAATAG TTTAATCAACCGGGTGTC NED 309-321 AAT 7 0.48 0.44 0.84 0.76
Sh9 GGGATGTATGCAGACTTG TTGTTTGGCTGCAGTAAC 6-FAM 197-227 AAT 11 0.46 0.76 0.46 0.86
Sh3 GCTGAGCTTGAGATTG CTTCTGTCCCATCGATACC 6-FAM 270-366 AAT 30 0.76 0.86 0.94 0.86
C111 CCCTTGTCTTCAATGCGTTA GAACGTCTAACTGGCGATCA PET 201-225 ATT 9 0.74 0.67 0.76 0.68
Sh7 TCCAAGCACCATTATCAAG ACGGAAACTTGTTGAAATG PET 293-311 AAT 7 0.46 0.62 0.42 0.48
Panel 2 Sh2 TTAGTGTGTTTGGCTTCAAC CCTCGAATGAAATCCTGAC NED 155-218 AAT 21 0.84 0.90 0.56 0.89
Sh5 TGTGCACAAGAAAGATTAAATG ACGACAATGTTGCAAGTTC NED 263-314 AAT 16 0.78 0.81 0.36 0.48
Sh13 GAGCAGCTATTTCGTATCG ACCGTGGACAGTTCATCAG 6-FAM 163-211 AAT 17 0.78 0.72 0.68 0.64
Sh4 CCCATCGCTGATATTAAAG TCTAGTCGTCTTGGGATCC 6-FAM 268-313 AAT 13 0.84 0.78 0.72 0.79
Sh10 CGCATGTCATACCTATCTCC GCTTATCAGGCCTATCTCC PET 183-207 AAT 9 0.18 0.34 0.74 0.70
Sh12 CGTCTTAGTGAGCCAGATG CTCGTGGACATCATCAG PET 245-278 AAC 11 0.06 0.06 0.56 0.65
Sh8 CTAAACTGGCAAGATTTC CAACGTGCCTTTATTTC PET 282-321 AAT 14 0.76 0.81 0.84 0.83
Sh11 TTGGTTTAGAAATTACATCACC CCAACAATATTAATGGACAGC VIC 183-213 ATC 9 0.68 0.58 0.68 0.69
Sh15 CTTTCAGTAGGATTTGTTG CGACGTCAAGCACTGTAC VIC 274-301 ATC 10 0.78 0.65 0.50 0.466

Panel = single mulitplex PCR. A = observed number of alleles. Dye = the fluorescent dye label of the forward primer (VIC = green, NED = yellow, 6-FAM = Blue, PET = red). Ho = observed heterozygosity, He = expected heterozygosity

A corrected version of Table 1 is included below.

Footnotes

The online version of the original article can be found under doi:10.1186/s13071-015-1044-6.

Reference

  • 1.Webster BL, Rabone M, Pennance T, Emery AM, Allan F, Gouvras S, et al. Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium. Parasit and Vectors. 2015;8:432. doi: 10.1186/s13071-015-1044-6. [DOI] [PMC free article] [PubMed] [Google Scholar]

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