Figure 4.

Effect of nucleotides and inhibitors on the Hsp90β-p50^Cdc37 complex as determined by ELISA. (A) Dose-response experiments for the complex formation between immobilized GST-p50^Cdc37 and Hsp90β (unphosphorylated or phosphorylated) in the presence of ADP, ATP, AMP-PNP, NVP-AUY922, gedunin, or H2-GMZ, as indicated; (B) Replot of (A) to compare the effect of phosphorylation on the stability of the complex. The Hsp90-(p)p50^Cdc37 complex was probed only against ADP and ATP but not against inhibitors. IC₅₀ values are shown in Table 2.