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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Apr 13;35(3):366–376. doi: 10.1038/onc.2015.91

Figure 4. Requirement for Runx2-S301, S319 phosphorylation in stimulation of prostate cell migration.

Figure 4

PC3 and RWPE1 cells were transduced with LacZ, Runx2-WT or Runx2-SA adenovirus vectors. After 48 h, cells were trypsinized and cell migration was measured as described in Methods. (A, C) PC3 cell migration. Cell images after 0 and 6 h (A). Percent occupancy of the initially cell-free area was determined after the indicated times (C). (B, D) RWPE1 cell migration. Images of cells after 0 or 24 h (B). Percent occupancy of the initially cell-free area was determined after the indicated times (D). Statistics: C, aSignificantly different from LacZ control (p < 0.001), bSignificantly different from Runx2-WT (p < 0.001); D, aSignificantly different from LacZ control (p < 0.05), bSignificantly different from Runx2-WT (p < 0.01), N = 4/group.