Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Thorax logoLink to Thorax
. 1987 Jan;42(1):19–25. doi: 10.1136/thx.42.1.19

Suppression of host defences by Aspergillus fumigatus.

M D Robertson, A Seaton, L J Milne, J A Raeburn
PMCID: PMC460598  PMID: 3039682

Abstract

An important feature of the microbicidal action of phagocytic cells is their ability to produce reactive oxygen intermediates. In an attempt to identify the mechanisms by which the fungus Aspergillus fumigatus resists normal host defences the effect of spores and spore diffusates of A fumigatus on the production of superoxide anion and hydrogen peroxide by primed rodent phagocytic cells has been measured. For comparison we have used the non-pathogenic fungus Penicillium ochrochloron. Production of these reactive oxygen intermediates in response to A fumigatus was significantly lower than that in response to P ochrochloron. A similar reduction was achieved by diffusate prepared from freshly washed spores. The inhibitory component was of low molecular weight (less than 14,000) and its effect was dose dependent. These results suggest that spores of A fumigatus fail to trigger and also inhibit the production of reactive oxygen intermediates by phagocytic cells.

Full text

PDF
19

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayars G. H., Altman L. C., Rosen H., Doyle T. The injurious effect of neutrophils on pneumocytes in vitro. Am Rev Respir Dis. 1984 Jun;129(6):964–973. doi: 10.1164/arrd.1984.129.6.964. [DOI] [PubMed] [Google Scholar]
  2. Babior B. M. Oxygen-dependent microbial killing by phagocytes (first of two parts). N Engl J Med. 1978 Mar 23;298(12):659–668. doi: 10.1056/NEJM197803232981205. [DOI] [PubMed] [Google Scholar]
  3. Buchanan D. R., Lamb D. Saprophytic invasion of infarcted pulmonary tissue by Aspergillus species. Thorax. 1982 Sep;37(9):693–698. doi: 10.1136/thx.37.9.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diamond R. D., Clark R. A. Damage to Aspergillus fumigatus and Rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro. Infect Immun. 1982 Nov;38(2):487–495. doi: 10.1128/iai.38.2.487-495.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donaldson K., Slight J., Hannant D., Bolton R. E. Increased release of hydrogen peroxide and superoxide anion from asbestos-primed macrophages. Effect of hydrogen peroxide on the functional activity of alpha 1-protease inhibitor. Inflammation. 1985 Jun;9(2):139–147. doi: 10.1007/BF00917586. [DOI] [PubMed] [Google Scholar]
  6. Glasgow L. A., Fischbach J., Bryant S. M., Kern E. R. Immunomodulation of host resistance to experimental viral infections in mice: effects of Corynebacterium acnes, Corynebacterium parvum, and Bacille calmette-guérin. J Infect Dis. 1977 May;135(5):763–770. doi: 10.1093/infdis/135.5.763. [DOI] [PubMed] [Google Scholar]
  7. Karnovsky M. L., Badwey J. A. Determinants of the production of active oxygen species by granulocytes and macrophages. J Clin Chem Clin Biochem. 1983 Sep;21(9):545–553. [PubMed] [Google Scholar]
  8. Kurup V. P. Interaction of Aspergillus fumigatus spores and pulmonary alveolar macrophages of rabbits. Immunobiology. 1984 Jan;166(1):53–61. doi: 10.1016/S0171-2985(84)80143-6. [DOI] [PubMed] [Google Scholar]
  9. Lehrer R. I., Jan R. G. Interaction of Aspergillus fumigatus Spores with Human Leukocytes and Serum. Infect Immun. 1970 Apr;1(4):345–350. doi: 10.1128/iai.1.4.345-350.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levitz S. M., Diamond R. D. Killing of Aspergillus fumigatus spores and Candida albicans yeast phase by the iron-hydrogen peroxide-iodide cytotoxic system: comparison with the myeloperoxidase-hydrogen peroxide-halide system. Infect Immun. 1984 Mar;43(3):1100–1102. doi: 10.1128/iai.43.3.1100-1102.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McLeod D. T., Milne L. J., Seaton A. Successful treatment of invasive pulmonary aspergillosis complicating influenza A. Br Med J (Clin Res Ed) 1982 Oct 23;285(6349):1166–1167. doi: 10.1136/bmj.285.6349.1166-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mullins J., Harvey R., Seaton A. Sources and incidence of airborne Aspergillus fumigatus (Fres). Clin Allergy. 1976 May;6(3):209–217. doi: 10.1111/j.1365-2222.1976.tb01899.x. [DOI] [PubMed] [Google Scholar]
  13. Mullins J., Seaton A. Fungal spores in lung and sputum. Clin Allergy. 1978 Sep;8(5):525–533. doi: 10.1111/j.1365-2222.1978.tb01506.x. [DOI] [PubMed] [Google Scholar]
  14. Murray H. W., Juangbhanich C. W., Nathan C. F., Cohn Z. A. Macrophage oxygen-dependent antimicrobial activity. II. The role of oxygen intermediates. J Exp Med. 1979 Oct 1;150(4):950–964. doi: 10.1084/jem.150.4.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Müllbacher A., Waring P., Eichner R. D. Identification of an agent in cultures of Aspergillus fumigatus displaying anti-phagocytic and immunomodulating activity in vitro. J Gen Microbiol. 1985 May;131(5):1251–1258. doi: 10.1099/00221287-131-5-1251. [DOI] [PubMed] [Google Scholar]
  16. Pick E., Keisari Y. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods. 1980;38(1-2):161–170. doi: 10.1016/0022-1759(80)90340-3. [DOI] [PubMed] [Google Scholar]
  17. Sasada M., Johnston R. B., Jr Macrophage microbicidal activity. Correlation between phagocytosis-associated oxidative metabolism and the killing of Candida by macrophages. J Exp Med. 1980 Jul 1;152(1):85–98. doi: 10.1084/jem.152.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schaffner A., Douglas H., Braude A. Selective protection against conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to Aspergillus. Observations on these two lines of defense in vivo and in vitro with human and mouse phagocytes. J Clin Invest. 1982 Mar;69(3):617–631. doi: 10.1172/JCI110489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Swartzberg J. E., Krahenbuhl J. L., Remington J. S. Dichotomy between macrophage activation and degree of protection against Listeria monocytogenes and Toxoplasma gondii in mice stimulated with Corynebacterium parvum. Infect Immun. 1975 Nov;12(5):1037–1043. doi: 10.1128/iai.12.5.1037-1043.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. White L. O. Germination of Aspergillus fumigatus conidia in the lungs of normal and cortisone-treated mice. Sabouraudia. 1977 Mar;15(1):37–41. [PubMed] [Google Scholar]

Articles from Thorax are provided here courtesy of BMJ Publishing Group

RESOURCES