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Time course of PTX-3 protein induction in fibrocytes by bTSH. Confluent cultures were incubated without or with bTSH (5 mIU/mL) for the intervals indicated along the abscissas. A, Medium was collected and subjected to a PTX-3–specific ELISA. Data are expressed as means ± SD of triplicates from 3 independent experiments. P < .05 compared with baseline. B, Cellular protein was extracted for Western blot analysis. C, Bands from B were quantified by densitometric analysis.
