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. 1987 Mar;42(3):183–189. doi: 10.1136/thx.42.3.183

Calcitonin gene related peptide immunoreactivity in rat lung: light and electron microscopic study.

J M Lauweryns, L van Ranst
PMCID: PMC460686  PMID: 3303426

Abstract

Calcitonin gene related peptide immunoreactivity was investigated in the rat lung. Immunoreactive material was observed in nerve fibres surrounding bronchi, bronchioli, and blood vessels, and also in neuroendocrine cells and neuroepithelial bodies. In addition, the peptide was identified by immunoelectron microscopy in dense cored vesicles of granulated cells of the neuroepithelial bodies. These observations suggest that calcitonin gene related peptide plays a part in the regulatory function of the rat respiratory tract, exerting its influence by endocrine, paracrine, neurosecretory pathways, or a combination of these.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amara S. G., Arriza J. L., Leff S. E., Swanson L. W., Evans R. M., Rosenfeld M. G. Expression in brain of a messenger RNA encoding a novel neuropeptide homologous to calcitonin gene-related peptide. Science. 1985 Sep 13;229(4718):1094–1097. doi: 10.1126/science.2994212. [DOI] [PubMed] [Google Scholar]
  2. Bendayan M., Zollinger M. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. J Histochem Cytochem. 1983 Jan;31(1):101–109. doi: 10.1177/31.1.6187796. [DOI] [PubMed] [Google Scholar]
  3. Clague J. R., Sternini C., Brecha N. C. Localization of calcitonin gene-related peptide-like immunoreactivity in neurons of the rat gastrointestinal tract. Neurosci Lett. 1985 May 1;56(1):63–68. doi: 10.1016/0304-3940(85)90441-0. [DOI] [PubMed] [Google Scholar]
  4. Cutz E., Chan W., Track N. S. Bombesin, calcitonin and leu-enkephalin immunoreactivity in endocrine cells of human lung. Experientia. 1981 Jul 15;37(7):765–767. doi: 10.1007/BF01967969. [DOI] [PubMed] [Google Scholar]
  5. Gibson S. J., Polak J. M., Bloom S. R., Sabate I. M., Mulderry P. M., Ghatei M. A., McGregor G. P., Morrison J. F., Kelly J. S., Evans R. M. Calcitonin gene-related peptide immunoreactivity in the spinal cord of man and of eight other species. J Neurosci. 1984 Dec;4(12):3101–3111. doi: 10.1523/JNEUROSCI.04-12-03101.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gosney J. R., Sissons M. C. Widespread distribution of bronchopulmonary endocrine cells immunoreactive for calcitonin in the lung of the normal adult rat. Thorax. 1985 Mar;40(3):194–198. doi: 10.1136/thx.40.3.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hanko J., Hardebo J. E., Kåhrström J., Owman C., Sundler F. Calcitonin gene-related peptide is present in mammalian cerebrovascular nerve fibres and dilates pial and peripheral arteries. Neurosci Lett. 1985 Jun 4;57(1):91–95. doi: 10.1016/0304-3940(85)90045-x. [DOI] [PubMed] [Google Scholar]
  8. Höppener J. W., Steenbergh P. H., Zandberg J., Geurts van Kessel A. H., Baylin S. B., Nelkin B. D., Jansz H. S., Lips C. J. The second human calcitonin/CGRP gene is located on chromosome 11. Hum Genet. 1985;70(3):259–263. doi: 10.1007/BF00273453. [DOI] [PubMed] [Google Scholar]
  9. Lauweryns J. M., Cokelaere M., Deleersynder M., Liebens M. Intrapulmonary neuro-epithelial bodies in newborn rabbits. Influence of hypoxia, hyperoxia, hypercapnia, nicotine, reserpine, L-DOPA and 5-HTP. Cell Tissue Res. 1977 Sep 5;182(4):425–440. doi: 10.1007/BF00219827. [DOI] [PubMed] [Google Scholar]
  10. Lauweryns J. M., Cokelaere M., Theunynck P. Neuro-epithelial bodies in the respiratory mucosa of various mammals. A light optical, histochemical and ultrastructural investigation. Z Zellforsch Mikrosk Anat. 1972;135(4):569–592. doi: 10.1007/BF00583438. [DOI] [PubMed] [Google Scholar]
  11. Lauweryns J. M., Liebens M. Microspectrography of formaldehyde and fluorescamine-induced fluorescence in rabbit pulmonary neuroepithelial bodies: demonstration of a new, probably polypeptide intracytoplasmic substance. Experientia. 1977 Nov 15;33(11):1510–1511. doi: 10.1007/BF01918840. [DOI] [PubMed] [Google Scholar]
  12. Lauweryns J. M., Peuskens J. C. Neuro-epithelial bodies (neuroreceptor or secretory organs?) in human infant bronchial and bronchiolar epithelium. Anat Rec. 1972 Mar;172(3):471–481. doi: 10.1002/ar.1091720301. [DOI] [PubMed] [Google Scholar]
  13. Lauweryns J. M., Van Lommel A. T., Dom R. J. Innervation of rabbit intrapulmonary neuroepithelial bodies. Quantitative and qualitative ultrastructural study after vagotomy. J Neurol Sci. 1985 Jan;67(1):81–92. doi: 10.1016/0022-510x(85)90024-3. [DOI] [PubMed] [Google Scholar]
  14. Lauweryns J. M., Van Lommel A. Morphometric analysis of hypoxia-induced synaptic activity in intrapulmonary neuroepithelial bodies. Cell Tissue Res. 1982;226(1):201–214. doi: 10.1007/BF00217094. [DOI] [PubMed] [Google Scholar]
  15. Lauweryns J. M., Van Ranst L., Verhofstad A. A. Ultrastructural localization of serotonin in the intrapulmonary neuroepithelial bodies of neonatal rabbits by use of immunoelectron microscopy. Cell Tissue Res. 1986;243(3):455–459. doi: 10.1007/BF00218051. [DOI] [PubMed] [Google Scholar]
  16. Lauweryns J. M., de Bock V., Verhofstad A. A., Steinbusch H. W. Immunohistochemical localization of serotonin in intrapulmonary neuro-epithelial bodies. Cell Tissue Res. 1982;226(1):215–223. doi: 10.1007/BF00217095. [DOI] [PubMed] [Google Scholar]
  17. Mason R. T., Peterfreund R. A., Sawchenko P. E., Corrigan A. Z., Rivier J. E., Vale W. W. Release of the predicted calcitonin gene-related peptide from cultured rat trigeminal ganglion cells. Nature. 1984 Apr 12;308(5960):653–655. doi: 10.1038/308653a0. [DOI] [PubMed] [Google Scholar]
  18. Rodrigo J., Polak J. M., Terenghi G., Cervantes C., Ghatei M. A., Mulderry P. K., Bloom S. R. Calcitonin gene-related peptide (CGRP)-immunoreactive sensory and motor nerves of the mammalian palate. Histochemistry. 1985;82(1):67–74. doi: 10.1007/BF00502092. [DOI] [PubMed] [Google Scholar]
  19. Rosenfeld M. G., Mermod J. J., Amara S. G., Swanson L. W., Sawchenko P. E., Rivier J., Vale W. W., Evans R. M. Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature. 1983 Jul 14;304(5922):129–135. doi: 10.1038/304129a0. [DOI] [PubMed] [Google Scholar]
  20. Sabate M. I., Stolarsky L. S., Polak J. M., Bloom S. R., Varndell I. M., Ghatei M. A., Evans R. M., Rosenfeld M. G. Regulation of neuroendocrine gene expression by alternative RNA processing. Colocalization of calcitonin and calcitonin gene-related peptide in thyroid C-cells. J Biol Chem. 1985 Mar 10;260(5):2589–2592. [PubMed] [Google Scholar]
  21. Seifert H., Sawchenko P., Chesnut J., Rivier J., Vale W., Pandol S. J. Receptor for calcitonin gene-related peptide: binding to exocrine pancreas mediates biological actions. Am J Physiol. 1985 Jul;249(1 Pt 1):G147–G151. doi: 10.1152/ajpgi.1985.249.1.G147. [DOI] [PubMed] [Google Scholar]
  22. Severs N. J. Inverted logic. 1984 Apr 26-May 2Nature. 308(5962):776–776. doi: 10.1038/308776b0. [DOI] [PubMed] [Google Scholar]
  23. Sikri K. L., Varndell I. M., Hamid Q. A., Wilson B. S., Kameya T., Ponder B. A., Lloyd R. V., Bloom S. R., Polak J. M. Medullary carcinoma of the thyroid. An immunocytochemical and histochemical study of 25 cases using eight separate markers. Cancer. 1985 Nov 15;56(10):2481–2491. doi: 10.1002/1097-0142(19851115)56:10<2481::aid-cncr2820561026>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  24. Varndell I. M., Tapia F. J., Probert L., Buchan A. M., Gu J., De Mey J., Bloom S. R., Polak J. M. Immunogold staining procedure for the localisation of regulatory peptides. Peptides. 1982 May-Jun;3(3):259–272. doi: 10.1016/0196-9781(82)90086-9. [DOI] [PubMed] [Google Scholar]
  25. Wasano K. Neuro-epithelial bodies in the lung of the rat and the mouse. Arch Histol Jpn. 1977;40 (Suppl):207–219. doi: 10.1679/aohc1950.40.supplement_207. [DOI] [PubMed] [Google Scholar]
  26. Wharton J., Polak J. M., Bloom S. R., Ghatei M. A., Solcia E., Brown M. R., Pearse A. G. Bombesin-like immunoreactivity in the lung. Nature. 1978 Jun 29;273(5665):769–770. doi: 10.1038/273769a0. [DOI] [PubMed] [Google Scholar]

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