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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Immunity. 2013 Jun 20;38(6):1271–1284. doi: 10.1016/j.immuni.2013.05.011

Figure 4.

Figure 4

Enhancer and STAT6 Motif Validation by ChIP-qPCR. (A) Time course of Th2 cell polarization and Th0 cells as a control. H3K4me1, H3K27ac and STAT6 localization were tested by ChIP-qPCR at 6 ChIP-seq determined loci of Th2 cell-specific H3K4me1 marked enhancers containing STAT6 motifs. (B) ChIP-qPCR for STAT6 binding at 4hrs and 72hrs in early stages of Th2 cell polarization. Th0 and Thp cells serve as controls. Enrichment is a percent of input. (C) As in A, except for H3K4me1. (D) As in A, except for H3K27ac. Error bars were represented as mean +/− SEM.