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. 1987 Apr;42(4):296–301. doi: 10.1136/thx.42.4.296

Transmission electron microscopy of percutaneous fine needle aspirates from lung: a study of 70 cases.

D C Davies, A J Russell, R Tayar, N T Cooke, M M Levene
PMCID: PMC460711  PMID: 3616988

Abstract

Seventy consecutive lung fine needle aspirates (FNA) from 69 patients were examined by cytology/cell block histology, by transmission electron microscopy (TEM), and, where available, at histological follow up to determine the value of TEM in interpreting lung FNAs. Of 70 FNAs, 50 were suitable for TEM. Transmission electron microscopy was helpful in classifying seven of 10 poorly differentiated tumours, and two case histories are reported. In 26 of 30 well differentiated tumours TEM confirmed diagnosis by light microscopy. Two TEM false negatives, but no TEM false positives, were recorded. It is concluded that transmission electron microscopy of cells aspirated from lung can be helpful when unequivocal diagnosis has not been achieved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akhtar M., Ali M. A., Owen E. W. Application of electron microscopy in the interpretation of fine-needle aspiration biopsies. Cancer. 1981 Dec 1;48(11):2458–2463. doi: 10.1002/1097-0142(19811201)48:11<2458::aid-cncr2820481119>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  2. Akhtar M., Ali M. A., Owen E., Bakry M. A simple method for processing fine-needle aspiration biopsy specimens for electron microscopy. J Clin Pathol. 1980 Dec;33(12):1214–1216. doi: 10.1136/jcp.33.12.1214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berkman W. A., Chowdhury L., Brown N. L., Padleckas R. Value of electron microscopy in cytologic diagnosis of fine-needle biopsy. AJR Am J Roentgenol. 1983 Jun;140(6):1253–1258. doi: 10.2214/ajr.140.6.1253. [DOI] [PubMed] [Google Scholar]
  4. Coleman D. V., Russell W. J., Hodgson J., Pe T., Mowbray J. F. Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy. J Clin Pathol. 1977 Nov;30(11):1015–1020. doi: 10.1136/jcp.30.11.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunnill M. S., Gatter K. C. Cellular heterogeneity in lung cancer. Histopathology. 1986 May;10(5):461–475. doi: 10.1111/j.1365-2559.1986.tb02498.x. [DOI] [PubMed] [Google Scholar]
  6. Fisher C., Ramsay A. D., Griffiths M., McDougall J. An assessment of the value of electron microscopy in tumour diagnosis. J Clin Pathol. 1985 Apr;38(4):403–408. doi: 10.1136/jcp.38.4.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hagelqvist E. Light and electron microscopic studies on material obtained by fine needle biopsy. A methodological study on aspirates from tumours of the head and neck region with special emphasis on salivary gland tumours. Acta Otolaryngol Suppl. 1978;354:1–75. [PubMed] [Google Scholar]
  8. Hajdu S. I., Melamed M. R. Limitations of aspiration cytology in the diagnosis of primary neoplasms. Acta Cytol. 1984 May-Jun;28(3):337–345. [PubMed] [Google Scholar]
  9. Kline T. S., Neal H. S. Needle aspiration biopsy: a critical appraisal. Eight years and 3,267 specimens later. JAMA. 1978 Jan 2;239(1):36–39. [PubMed] [Google Scholar]
  10. Leong A. S. The relevance of ultrastructural examination in the classification of primary lung tumours. Pathology. 1982 Jan;14(1):37–46. doi: 10.3109/00313028209069041. [DOI] [PubMed] [Google Scholar]
  11. Mennemeyer R., Hammar S. P., Bauermeister D. E., Wheelis R. F., Jones H. W., Bartha M. Cytologic, histologic and electron microscopic correlations in poorly differentiated primary lung carcinoma. A study of 43 cases. Acta Cytol. 1979 Jul-Aug;23(4):297–302. [PubMed] [Google Scholar]
  12. Payne C. R., Stovin P. G., Barker V., McVittie S., Stark J. E. Diagnostic accuracy of cytology and biopsy in primary bronchial carcinoma. Thorax. 1979 Jun;34(3):294–299. doi: 10.1136/thx.34.3.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pilotti S., Rilke F., Gribaudi G., Damascelli B. Fine needle aspiration biopsy cytology of primary and metastatic pulmonary tumors. Acta Cytol. 1982 Sep-Oct;26(5):661–666. [PubMed] [Google Scholar]
  14. Pilotti S., Rilke F., Gribaudi G., Damascelli B., Ravasi G. Transthoracic fine needle aspiration biopsy in pulmonary lesions. Updated results. Acta Cytol. 1984 May-Jun;28(3):225–232. [PubMed] [Google Scholar]
  15. Sehested M., Francis D., Hainau B. Electron microscopy of trans-thoracic fine needle aspiration biopsies. Acta Pathol Microbiol Immunol Scand A. 1983 Nov;91(6):457–461. doi: 10.1111/j.1699-0463.1983.tb02778.x. [DOI] [PubMed] [Google Scholar]
  16. Stevens G. M., Jackman R. J. Outpatient needle biopsy of the lung: its safety and utility. Radiology. 1984 May;151(2):301–304. doi: 10.1148/radiology.151.2.6709896. [DOI] [PubMed] [Google Scholar]
  17. Thornbury J. R., Burke D. P., Naylor B. Transthoracic needle aspiration biopsy: accuracy of cytologic typing of malignant neoplasms. AJR Am J Roentgenol. 1981 Apr;136(4):719–724. doi: 10.2214/ajr.136.4.719. [DOI] [PubMed] [Google Scholar]
  18. Wang N. S. Applications of electron microscopy to diagnostic pulmonary pathology. Hum Pathol. 1983 Oct;14(10):888–900. doi: 10.1016/s0046-8177(83)80164-6. [DOI] [PubMed] [Google Scholar]
  19. Wilson T. S., McDowell E. M., Marangos P. J., Trump B. F. Histochemical studies of dense-core granulated tumors of the lung. Neuron-specific enolase as a marker for granulated cells. Arch Pathol Lab Med. 1985 Jul;109(7):613–620. [PubMed] [Google Scholar]
  20. Wilson T. S., McDowell E. M., Trump B. F. Immunohistochemical studies of keratin in human bronchus and lung tumors. Arch Pathol Lab Med. 1985 Jul;109(7):621–628. [PubMed] [Google Scholar]

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