Extended Data Table 1. Statistics of structure determination.
| a. Statistics of APC/CCdh1.Emi1 structure determination | |
|---|---|
| Data collection | |
| EM: | FEI Polara, 300keV |
| Detector: | FEI Falcon II |
| Pixel size (Å): | 1.36 |
| Defocus range (μm): | 1.5-4 |
| Reconstruction | |
| Software: | RELION 1.3 |
| Accuracy of rotations (degrees): | 1.073 |
| Accuracy of translations (pixels): | 0.691 |
| Final Resolution (Å): | 3.6 |
| Refinement | |
| Software: | RefMac 5.8 |
| Refmac weight: | 0.04 |
| Resolution limits (Å): | 3.5 |
| Residue number | 8408 |
| Average Fourier shell correlation | 0.8315 |
| R factor: | 0.222 |
| Rms BondLength: | 0.0135 |
| Rms BondAngle: | 1.8098 |
| Validation | |
| Ramachandran plot: | |
| Preferred: | 7669 (92.88%) |
| Allowed: | 352 (4.26%) |
| Outliers: | 236 (2.86%) |
| b. Statistics of all cryo-EM data | ||||
|---|---|---|---|---|
| Samples | Micrographs | Particles (after movie correction) | Resolution (Å) | |
| APC/CCdh1.Emi1 | 3328 | In total:363,774 | - | |
| Ternary | 202,084 (55.6%) | 3.6 | ||
| Apo | 161,690 (44.4%) | 4.3 | ||
| *Others (Hybrid, Weak Apc2 and Bad particles) |
*52,178 particles deleted before movie correction |
- | ||
| APC/CCdh1.Hsl1-UbcH10RL~Ub | 4524 | In total: 477,850 | - | |
| Ternary | 282,440 (59.1%) | 4.1 | ||
| Apo | 56,865 (11.9%) | - | ||
| Others (Hybrid, Weak Apc2 and Bad particles) |
138,545 (29.0%) | - | ||
| APC/CCdh1.Hsl1-UbcH10Apc11-UbcH10 | 869 | In total: 97,999 | - | |
| Ternary | 79,004 (80.6%) | 4.3 | ||
| Apo | 9,114 (9.3%) | - | ||
| Others (Hybrid, Weak Apc2 and Bad particles) |
9,801 (10.1%) | - | ||