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Analytical Cellular Pathology: the Journal of the European Society for Analytical Cellular Pathology logoLink to Analytical Cellular Pathology: the Journal of the European Society for Analytical Cellular Pathology
. 1998 Jan 1;16(3):125–130. doi: 10.1155/1998/705216

Detection of Human Papillomavirus Type 2 Related Sequence in Oral Papilloma

Taihei Yamaguchi 1,2,5, Masanobu Shindoh 3,5, Akira Amemiya 3, Nobuo Inoue 4, Masaaki Kawamura 4, Hiroshi Sakaoka 2, Masakazu Inoue 1, Kei Fujinaga 5
PMCID: PMC4611164  PMID: 9699941

Abstract

Oral papilloma is a benign tumourous lesion. Part of this lesion is associated with human papillomavirus (HPV) infection. We analysed the genetical and histopathological evidence for HPV type 2 infection in three oral papillomas. Southern blot hybridization showed HPV 2a sequence in one lesion. Cells of the positive specimen appeared to contain high copy numbers of the viral DNA in an episomal state. In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. Two other specimens contained no HPV sequences by labeled probe of full length linear HPVs 2a, 6b, 11, 16, 18, 31 and 33 DNA under low stringency hybridization conditions. These results showed the possibility that HPV 2 plays a role in oral papilloma.

Keywords: Human papillomavirus type 2, oral papilloma, southern blot hybridization


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