Correction to: Cell Death and Disease (2013) 4, e641; doi:10.1038/cddis.2013.165; published online 23 May 2013
Since the publication of this paper the authors have noticed the blots in Figure 5c displaying p-PHSP27 and HSP27 were incorrect. The correct figure is shown below. The corrected article appears online together with this corrigendum.
Figure 5.
P38-MAPK activation was involved in the resistance to ZOL of DU145R80 cells. (a) Analysis of p38-MAPK and HSP27 activation evaluated by a phosphoprotein ELISA-based immunoassay (see Materials and Methods) (a and b) and by western blot (a and b inset panels). Values from phosphoprotein assay are means±S.D. of two independent experiments performed in triplicates and statistical analysis of DU145R80 versus DU145 cells is reported (*P=0.037, p38-MAPK; P=0.005, HSP27). (c) DU145R80 cells were treated for 96 h with increasing concentrations of ZOL alone or after 24 h of pretreatment with SB 30 μM and compared with DU145 treated with ZOL alone. Cell growth expressed as percentage of control was assessed by sulforhodamine B colorimetric assay (see Materials and Methods) and each point is the mean±S.D. of three independent experiments. Expression of p-HSP27 and HSP-27 in DU145R80 cells untreated or treated with SB 30 μM for 24 h were evaluated by western blot (inset panel). (d) Apoptosis evaluated by AnnexinV binding and cytofluorimetric analysis in DU145R80 cells untreated or treated with ZOL 20 μM alone or in combination with SB 30 μM for 48 h. Values of apoptotic cells are means±S.D. of three independent experiments and were expressed as per cent of untreated cells (100%). Statistical analysis demonstrated significant differences only in ZOL+SB combination versus untreated cells (*P=0.026). (e) Soft-agar clonogenic assay was performed on DU145 and DU145R80 cells untreated or treated with ZOL alone (20 μM) or in combination with SB (30 μM) for 21 days, in 24-well plates. Colonies >100 μm were scored by a colony counter. Left: images from a representative experiment; right: values expressed as number of colonies are means±S.D. from at least two independent experiments performed in triplicates. Statistical analysis results are reported (*P<0.001, ZOL versus untreated cells in DU145; P=0.005, ZOL versus untreated cells in DU145R80; P=0.002, P=0.005, P=0.003, ZOL+SB combination versus untreated, versus ZOL or versus SB-treated cells, respectively, in DU145R80)
The authors would like to apologize for any inconvenience.