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. 2015 Oct 13;3:e1305. doi: 10.7717/peerj.1305

Figure 2. Inhibition of tyrosinase activity by E. characias leaves extracts.

Figure 2

Lineweaver-Burk plots for inhibition of extracts on mushroom tyrosinase activity using l-DOPA as substrate. Reaction mixtures (1 mL) contained mushroom tyrosinase in 25 mM phosphate buffer (pH 6.8) and l-DOPA and extracts at different concentrations. (A) Aqueous extract: the concentration used were 0 (●), 0.05 mg/mL (○), 0.1 mg/mL (▴) and 0.25 mg/mL (▵). (B) Ethanolic extract: the concentration used were 0 (●), 0.05 mg/mL (○), 0.1 mg/mL (▴) and 0.15 mg/mL (▵).