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. 2015 Jan 20;6(1):40–54. doi: 10.1080/19491034.2015.1004260

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Michal Schwartz, Anna Travesa, Steven W Martell, and Douglass J Forbes

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Nucleoporins anchored to the LacO array recruit other nucleoporins from the same subcomplex. Immunofluorescence microscopy of U2OS 2–6–3 cells transiently transfected with either LacI-CFP-Nup53 (A, B), LacI-CFP-Nup155 (C, D), or LacI-CFP-Nup58 (E, F) were stained with the indicated antibodies. Chromatin is visualized using DAPI stain. In panels (A) and (B), LacI-CFP-Nup53 was detected by CFP fluorescence. The right hand panels show a merged image of the 3 stains. The smaller insets show a magnified image of the LacO array in each image. Colocalization of the untagged endogenous Nup in question (middle panels) with the transfected LacI-CFP-Nup being tested (left panels) is summarized at the right.