Skip to main content
NCBI home page
Thorax logoLink to Thorax
. 1988 Dec;43(12):1007–1010. doi: 10.1136/thx.43.12.1007

Effect of aerosol challenge with sensitising antigen on the permeability of the surface of the rat trachea in life.

S Mukherjee 1, P Heap 1, F Carswell 1
PMCID: PMC461617  PMID: 3238630

Abstract

A rat model of immediate pulmonary hypersensitivity was used to investigate the permeability changes in the tracheal epithelium produced by aerosol challenge with antigen. The rats were sensitised by the intraperitoneal injection of antigen (dinitrophenyl (DNP19) ovalbumin). Sensitised and control animals were then challenged for 60 minutes with an aerosol of the same antigen, which also contained the electron dense pore marker lanthanum. Histological examination and x ray probe microanalysis showed a greater intercellular concentration of lanthanum in the tracheal epithelium in sensitised than in control animals. The results show that in sensitised rats increased intercellular penetrance of antigen can occur after antigen challenge.

Full text

PDF
1007

Images in this article

1008Image
on p.1008
1009Image
on p.1009

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrowcliffe M. P., Jones J. G. Solute permeability of the alveolar capillary barrier. Thorax. 1987 Jan;42(1):1–10. doi: 10.1136/thx.42.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boucher R. C., Johnson J., Inoue S., Hulbert W., Hogg J. C. The effect of cigarette smoke on the permeability of guinea pig airways. Lab Invest. 1980 Jul;43(1):94–100. [PubMed] [Google Scholar]
  3. Boucher R. C., Pare P. D., Gilmore N. J., Moroz L. A., Hogg J. C. Airway mucosal permeability in the Ascaris suum-sensitive rhesus monkey. J Allergy Clin Immunol. 1977 Aug;60(2):134–140. doi: 10.1016/0091-6749(77)90039-2. [DOI] [PubMed] [Google Scholar]
  4. Boucher R. C., Pare P. D., Hogg J. C. Relationship between airway hyperreactivity and hyperpermeability in Ascaris-sensitive monkeys. J Allergy Clin Immunol. 1979 Sep;64(3):197–201. doi: 10.1016/0091-6749(79)90095-2. [DOI] [PubMed] [Google Scholar]
  5. Carswell F., Oliver J. Respiratory functional changes on intratracheal challenge of sensitized rats. Respiration. 1985;47(2):138–150. doi: 10.1159/000194760. [DOI] [PubMed] [Google Scholar]
  6. Carswell F., Oliver J. Site of respiratory reaction in allergic rats challenged via the airways. Int Arch Allergy Appl Immunol. 1978;57(4):358–363. doi: 10.1159/000232125. [DOI] [PubMed] [Google Scholar]
  7. Carswell F., Oliver J. The respiratory response in sensitized rats to aerosol challenge. Immunology. 1978 Mar;34(3):465–470. [PMC free article] [PubMed] [Google Scholar]
  8. Chandler J. A. Recent developments in analytical electron microscopy. J Microsc. 1973 Aug;98(3):359–378. doi: 10.1111/j.1365-2818.1973.tb03840.x. [DOI] [PubMed] [Google Scholar]
  9. Elwood R. K., Kennedy S., Belzberg A., Hogg J. C., Paré P. D. Respiratory mucosal permeability in asthma. Am Rev Respir Dis. 1983 Sep;128(3):523–527. doi: 10.1164/arrd.1983.128.3.523. [DOI] [PubMed] [Google Scholar]
  10. Empey D. W., Laitinen L. A., Jacobs L., Gold W. M., Nadel J. A. Mechanisms of bronchial hyperreactivity in normal subjects after upper respiratory tract infection. Am Rev Respir Dis. 1976 Feb;113(2):131–139. doi: 10.1164/arrd.1976.113.2.131. [DOI] [PubMed] [Google Scholar]
  11. Hogg J. C., Paré P. D., Boucher R. C. Bronchial mucosal permeability. Fed Proc. 1979 Feb;38(2):197–201. [PubMed] [Google Scholar]
  12. Hulbert W. M., McLean T., Hogg J. C. The effect of acute airway inflammation on bronchial reactivity in guinea pigs. Am Rev Respir Dis. 1985 Jul;132(1):7–11. doi: 10.1164/arrd.1985.132.1.7. [DOI] [PubMed] [Google Scholar]
  13. Mezey R. J., Cohn M. A., Fernandez R. J., Januszkiewicz A. J., Wanner A. Mucociliary transport in allergic patients with antigen-induced bronchospasm. Am Rev Respir Dis. 1978 Oct;118(4):677–684. doi: 10.1164/arrd.1978.118.4.677. [DOI] [PubMed] [Google Scholar]
  14. Morrissey J. F., Carswell F. The fate of inhaled antigen within the respiratory tract of sensitized and unsensitized rats. Monogr Allergy. 1983;18:226–230. [PubMed] [Google Scholar]
  15. Mukherjee S., Heap P., Carswell F. Lanthanum penetration of the trachea after the immediate response of sensitized rats to aerosol antigen. Clin Exp Immunol. 1986 Sep;65(3):647–653. [PMC free article] [PubMed] [Google Scholar]
  16. Murlas C. G., Roum J. H. Sequence of pathologic changes in the airway mucosa of guinea pigs during ozone-induced bronchial hyperreactivity. Am Rev Respir Dis. 1985 Mar;131(3):314–320. doi: 10.1164/arrd.1985.131.3.314. [DOI] [PubMed] [Google Scholar]
  17. Salvato G. Some histological changes in chronic bronchitis and asthma. Thorax. 1968 Mar;23(2):168–172. doi: 10.1136/thx.23.2.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shaklai M., Tavassoli M. Lanthanum as an electron microscopic stain. J Histochem Cytochem. 1982 Dec;30(12):1325–1330. doi: 10.1177/30.12.6185564. [DOI] [PubMed] [Google Scholar]

Articles from Thorax are provided here courtesy of BMJ Publishing Group

RESOURCES