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. 2015 May 8;3:298. Originally published 2014 Dec 8. [Version 2] doi: 10.12688/f1000research.5919.2

Figure 5. Mean explant weight changes (mg) over incubation times from PS-2, HF205 and HF210.

Figure 5.

After surface sterilization and dissection, inner vegetative buds from three genotypes were placed on either mDCR or mDCR+MES medium at each initial pH level (3.6, 5.1, 5.7, 6.3, and 7.8) for incubation up to 42 days. These buds were incubated in a growth chamber at 25°C with light regime adjusted to 16-hour light followed by 8-hour darkness each day. Bud weight change was recorded using an electronic scale when sample reached each incubation time point. Vertical bars represent mean weight change (mg)±S.E. Sample sizes according to the incubation time (1–42 days) were for HF205, n=45, 15, 15, 30, 30, 20, 20, and 30, for HF210, n=30, 30, 30, 30, 30, 31, 30, 38, and 15, and for PS-2, n=15, 30, 30, 30, 30, 16, 33, and 6. Means sharing the same letter indicate non-significant difference between means ( P>0.05). Tukey’s (HSD) multiple comparison was used. Please see Dataset 4 for the raw data.