Erratum

The authors of “Oxidation of 7-dehydrocholesterol and desmosterol by human cytochrome P450 46A1” (J. Lipid Res. 2014. 55: 1933–1943) have advised that there were several errors in this article. There was an error in calculating the K_m values in Table 1 that led to subsequent errors in the k_cat/K_m values in the same table. The correct K_m and k_cat/K_m values were calculated and are shown in the corrected Table 1.

TABLE 1.

Kinetic parameters measured for 7-dehydrocholesterol, desmosterol, and cholesterol

Substrate	Product	kcat (pmol nmol−1 min−1)	Km (μM)	kcat/Km (mM−1 min−1)
7-Dehydrocholesterol	24-hydroxy-7-dehydrocholesterol	24 ± 1	0.24 ± 0.008	100 ± 5
	25-hydroxy-7-dehydrocholesterol	∼110	—	—
Desmosterol	24S,25-epoxycholesterol	33 ± 1	2.17 ± 0.28	15 ± 2
	27-hydroxydesmosterol	∼ 44	—	—
Cholesterol	24-hydroxycholesterol	59 ± 6	1.52 ± 0.21	39 ± 6.6

PAGE 1938:

“The calculated k_cat and K_M values for the formation of 24-hydroxy-7-dehydrocholesterol (from 7-dehydrocholesterol) were 24 ± 1 pmol nmol⁻¹ min⁻¹ and 2.8 ± 0.02 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).” should read, “The calculated k_cat and K_M values for the formation of 24-hydroxy-7-dehydrocholesterol (from 7-dehydrocholesterol) were 24 ± 1 pmol nmol⁻¹ min⁻¹ and 0.24 ± 0.008 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).”

“The k_cat and K_M values for 24S,25-epoxycholesterol (from desmosterol) were 33 ± 1 pmol nmol⁻¹ min⁻¹ and 9.4 ± 1 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).” should read, “The k_cat and K_M values for 24S,25-epoxycholesterol (from desmosterol) were 33 ± 1 pmol nmol⁻¹ min⁻¹ and 2.17 ± 0.28 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).”

PAGE 1939:

“The enzyme efficiency (k_cat/K_m) was similar for 7-dehydrocholesterol compared with cholesterol but 2–3 times higher than that of desmosterol.” should read, “The enzyme efficiency (k_cat/K_m) for 7-dehydrocholesterol was higher by two–three times compared to cholesterol and six–seven times higher than that of desmosterol.”

These errors do not affect the overall results or conclusions of this work.