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Analytical Cellular Pathology: the Journal of the European Society for Analytical Cellular Pathology logoLink to Analytical Cellular Pathology: the Journal of the European Society for Analytical Cellular Pathology
. 2000 Jan 1;20(2-3):69–82. doi: 10.1155/2000/495102

DNA Ploidy and Chromosomal Imbalances in Invasive Ductal Breast Cancer. A Comparative Study of DNA Image Cytometry and Comparative Genomic Hybridization (CGH)

Katrin Friedrich 1,*, Jens Scheithauer 1, Volker Dimmer 1, Wolfdietrich Meyer 1, Franz Theissig 1, Gunter Haroske 1, Klaus Dietmar Kunze 1
PMCID: PMC4618430  PMID: 11153610

Abstract

Chromosomal imbalances were analyzed in 62 breast cancers with different DNA ploidy by CGH. The results of DNA image cytometry and CGH are consistent with peridiploid and aneuploid cases. The peritetraploid tumors harbored a high number of chromosomal imbalances, as a hint for an unfavorable prognosis. The quantitative analysis of imbalances highlighted the role of different physical constituents of the chromosome, and of chromosomal losses in different DNA ploidy groups. The peritetraploid and aneuploid tumors differed from the peridiploid tumors in losses at 8p and 18q. The peritetraploid cancers exhibited more gains at 8q, the aneuploid tumors more losses at 17p than their peridiploid counterparts. The aneuploid cases differed from the peritetraploid tumors in a higher number of losses at 11q and 14q. Combinations of imbalances provide further insights into the genetic background of DNA ploidy. Hypotheses for the progression from peridiploid to nondiploid breast cancers are given.

Figures on http://www.esacp.org/acp/2000/20-2_3/friedrich.htm.

Keywords: Breast cancer, chromosomal imbalances, DNA ploidy, CGH, tumor progression


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