Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation

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(Stem Cell Reports 4, 61–73; January 13, 2015)

We regret that there were inadvertent errors in the Supplemental Information (Table S5 and S6) in our recent article. Several primer sequences were misplaced during copying from the source, two gene symbols (used as primer names) were misspelled, and two GenBank Accession numbers were also wrong. We apologize for any inconvenience this may have caused. Fortunately, the errors listed above did not affect the PCR reactions (as the correct primers were used), the results or conclusions drawn from the experiments.

The combined list of corrections appears below, and the Supplemental Information files have been updated online.

Corrections for Table S5: Primers for qRT-PCR

S/N	Primer Name	Sequence (5’-3’) (Correct)	Sequence (5’-3’) (Wrong)
1	18s_F	AATCGCATTTGCCATCACCG	TCGCTAGTTGGCATCGTTTATG
2	18s_R	TCACCACCCTCTCAACCTCA	CGGAGGTTCGAAGACGATCA
181	rfx6_ F	AAGGTGTTTGTCTGCCACGG	TGCGTGTCGAAGTAGTTCCC
182	rfx6_R	TCTGGTTCCAAGCCTTCGTG	ACAGGTTCAGTATGTGCAGGAG
227	traf2a_F	ACAGCACTGCAGGAGGTTTTA	GAACACCAAGGAGTCCCTCAG
239	zp2_F	CCCACAGTCTCTGTCTCCTGTGC	CGCGTCTATCCAGTGCTCTC
240	zp2_R	TTCCTTGCGCAACTCTGCTCAC	GGAGTAAACGGCCTGGGTG
	Primer Name (Correct)	Primer Name (Wrong)
93	henmt1_F	hemnt1_F
94	henmt1_R	hemnt1_R

Corrections for Table S6: Differential Expression Level of a Selected Set of Genes in Wild Type Individuals (WT) and PGC-Depleted Morphants (MO) Examined by Microarray and qRT-PCR at 14 and 22 dpf

S/N	Gene Symbol	Accession Number (Correct)	Accession Number (Wrong)
12	cyb5r1	NM_200189.2	NM_212685
80	rfx6	XM_679861.5	NM_001080048.1