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. 2015 Jul 17;4(5):764–773. doi: 10.1002/mbo3.277

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© 2015 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Polyester suture loops and porcelain penicylinders were prepared and tested according to AOAC Official Method 966.04 (See “Delayed Heat-Shock”) and a modified version (See “Immediate Heat-Shock”). Spores from B. subtilis had an exposure time of 4 h when tested against CIDEX™, whereas spores from C. sporogenes had an exposure time of 1 h when tested against CIDEX™. Regardless of spore species, the exposure times for STERIPLEX™ HC and STERIPLEX™ Ultra were 25 min and 15 min, respectively. Exposure times were determined experimentally by previous testing (data not shown) to be at the end of the kill curve for each spore species-disinfectant combination. Thirty polyester suture loops and 30 porcelain penicylinders were tested, for each spore type, disinfectant, and heat-treatment combination.