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. 2015 May 11;6(21):18518–18533. doi: 10.18632/oncotarget.3934

Figure 1. CD44 expression in parental and drug-resistant CRC cells.

Figure 1

A. Western blot analysis of CD44 expression in parental and drug-resistant HCT-116 (upper panel) or HT-29 cells (lower panel) using an antibody that detects all CD44 isoforms. CD44s, standard form of CD44; CD44v, variant forms of CD44. Actin was used as loading control. B. RNA was extracted from parental and drug-resistant cells and analyzed by qRT-PCR using primers that detect all isoforms of CD44 followed by agarose gel electrophoresis. C. RNA was extracted from parental and drug-resistant cells and analyzed by qRT-PCR using primers that specifically detect the CD44v3 or CD44v6 isoforms. Data were derived from three independent experiments. Columns correspond to the relative mRNA expression of the indicated mRNA normalized to GAPDH. Bars, SD. **p < 0.01; ***p < 0.001 as determined by Student's t-test.