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. 2015 Oct 27;2:150058. doi: 10.1038/sdata.2015.58

Population genomic datasets describing the post-vaccine evolutionary epidemiology of Streptococcus pneumoniae

Nicholas J Croucher 1,a, Jonathan A Finkelstein 2,3, Stephen I Pelton 4, Julian Parkhill 5, Stephen D Bentley 5, Marc Lipsitch 6, William P Hanage 6
PMCID: PMC4622223  PMID: 26528397

Abstract

Streptococcus pneumoniae is common nasopharyngeal commensal bacterium and important human pathogen. Vaccines against a subset of pneumococcal antigenic diversity have reduced rates of disease, without changing the frequency of asymptomatic carriage, through altering the bacterial population structure. These changes can be studied in detail through using genome sequencing to characterise systematically-sampled collections of carried S. pneumoniae. This dataset consists of 616 annotated draft genomes of isolates collected from children during routine visits to primary care physicians in Massachusetts between 2001, shortly after the seven valent polysaccharide conjugate vaccine was introduced, and 2007. Also made available are a core genome alignment and phylogeny describing the overall population structure, clusters of orthologous protein sequences, software for inferring serotype from Illumina reads, and whole genome alignments for the analysis of closely-related sets of pneumococci. These data can be used to study both bacterial evolution and the epidemiology of a pathogen population under selection from vaccine-induced immunity.

Subject terms: Bacterial genetics, Molecular evolution, Genetic variation, Respiratory tract diseases

Background & Summary

Streptococcus pneumoniae (the pneumococcus) is a genetically diverse bacterial species commonly asymptomatically carried in the nasopharynx of infants, an age group not capable of generating a strong adaptive immune response to the polysaccharide capsule that envelopes most pneumococcal cells1. This capsule inhibits immune clearance by both complement- and neutrophil-mediated pathways2, and is a critical factor in allowing S. pneumoniae to invade other anatomical sites and cause disease such as pneumonia, bacteraemia and meningitis, particularly in both the young and elderly. Consequently, polysaccharide conjugate vaccines (PCVs) based on the pneumococcal capsule have been developed to induce anti-pneumococcal immunity in children3. These vaccine formulations are limited in the number of antigens they contain: the first licensed formulation (PCV7) contained seven serotypes. However, over 90 immunologically distinct capsule polysaccharides (serotypes) have been identified in S. pneumoniae 4, which is the consequence of extensive genetic variation at the capsule polysaccharide synthesis (cps) locus. Hence at the point of vaccine introduction the pneumococcal population consists of a mix of ‘vaccine serotypes’, susceptible to artificially-induced host immunity, and ‘non-vaccine serotypes’. Nevertheless, the widespread use of PCVs has caused a substantial fall in the incidence of invasive pneumococcal disease5.

This is the consequence of the PCV7 vaccine having at least 50% efficacy in preventing nasopharyngeal carriage of vaccine serotypes6,7, and around 98% (ref. 8) efficacy against invasive disease caused by the same types. However, the overall levels of pneumococcal carriage have not changed post-vaccination owing to serotype replacement9,10: the increase in frequency of non-vaccine serotypes. The reduced incidence of pneumococcal disease has therefore been attributed to the lower rate at which these non-vaccine serotypes cause symptomatic infections relative to vaccine serotypes11. Additionally, as many multidrug-resistant pneumococci were of PCV7 serotypes prior to the vaccine’s introduction, it was anticipated that PCV7 would decrease the levels of S. pneumoniae antimicrobial resistance; however, any such benefit in this regard observed shortly after vaccine introduction12 was not sustained over the longer term13. Hence understanding how the carried pneumococcal population structure changes following the implementation of partial coverage PCVs is important for relating the intervention to its subsequent clinical outcomes.

To address this question, the carried population of pneumococci in Massachusetts has been followed by the Streptococcus pneumoniae Antimicrobial Resistance in Children (SPARC) project. The instigation of this study coincided with the introduction of PCV7 in the USA in 2000. Samples were obtained through swabbing the nasopharynx of children seven years of age or under during routine visits to primary care physicians14. In the spring of 2001, winter and spring of 2004, and winter and spring of 2006–2007, 742, 994 and 972 individuals were sampled, respectively13–15. The detected level of pneumococcal colonisation remained stable over these winters, with 190 (26% prevalence), 232 (23% prevalence) and 294 (30% prevalence) S. pneumoniae isolates recovered in the three successive sampling periods. This collection has been used to analyse the changing antigenic profile of the population in response to vaccine-induced immunity through serological typing13–15. The same collection was also genotyped by multilocus sequence typing16 (MLST) to relate these changes to the elimination, emergence and diversification of individual bacterial lineages17,18. Subsequently, whole genome sequencing was applied to the collection to investigate the population dynamics in greater detail19. This dataset consists of 616 annotated draft S. pneumoniae genomes representing the evolutionary epidemiology of the species as the population structure changed in response to vaccine-induced selection pressures. To aid analysis of the overall set of isolates, the species-wide core genome alignment and phylogeny are also made available, as are the predicted protein sequences, a method for inferring serotype from Illumina reads, and whole genome alignments for fifteen sets of closely-related isolates.

Methods

Culturing and phenotyping of strains

Following retrieval from storage, all bacterial samples were colony purified, then grown on 5% sheep’s blood agar overnight at 37 °C in the presence of 5% CO2. Samples were serologically typed using latex agglutination (Statens Serum Institut, Copenhagen) as a check on sample handling. Discrepant results with previous typing were verified using the Quellung reaction (Statens Serum Institut, Copenhagen; Table 1 (available online only)).

Table 1. Sample handling integrity and assembly properties.

Isolate Identification
  Sequence Access
Serology
Genotyping
Genomics Assembly & Annotation Information
Isolate Name Taxon ID Sample Accession Sequence Reads Accession First Contig Annotation Accession Final Contig Annotation Accession Original Serotype Retyping Results (if inconsistent) Serotype From Reads Original Sequence Type Sequence Type From Reads Sequence Type From Assembly Sequence Cluster Total Sequencing Yield (kb) Number of Contigs Assembly N50 Number of Coding Sequences Number of tRNAs Number of rRNAs
For each sample, the results of serological typing before storage (using the Quellung reaction), after revival (using latex agglutination) and after sequencing (inferred from Illumina read mapping) are tabulated. Similarly, the results of genotyping are shown following the original isolation (using capillary sequencing), after re-isolation and whole genome sequencing (inferred from Illumina read mapping) and after de novo assembly (using loci extracted from the assembled contigs). These are used as a check on the integrity of sample handling and processing. The properties of the final draft assemblies are also displayed, including the number of contigs and annotated sequence features they contain.                                    
1334 001334 ERS044037 ERR069731 CNST02000001 CNST02000153 10   10A 585 585 585 1 722874 153 62775 2000 45 3
279388 279388 ERS044115 ERR069809 CNUR02000001 CNUR02000219   10 10A   97 97 1 695458 219 43531 1998 38 3
CH2079 5Z52R ERS069979 ERR129088 COCE02000001 COCE02000187 10A   10A 816 816 816 1 553408 187 52304 1973 36 3
LE4000 N5O68 ERS070017 ERR129126 CFKD02000001 CFKD02000152 10A   10A 3290 3290 3290 1 577924 152 54788 1963 35 3
LE4124 RUJ90 ERS070049 ERR129158 COEA02000001 COEA02000141 10A   10A 816 816 816 1 521438 141 77373 1976 45 3
MD5021 29ORI ERS070055 ERR129164 COEG02000001 COEG02000169 10A   10A 816 816 816 1 537572 169 55810 2024 43 3
ND6034 RN3X8 ERS070090 ERR129199 COFG02000001 COFG02000132 10A   10A 816 816 816 1 539173 132 69832 1972 43 3
ND6039 PKMM4 ERS070092 ERR129201 CFRQ02000001 CFRQ02000122 10A   10A 97 97 97 1 528497 122 82155 1998 45 3
NP7052 N6I98 ERS070147 ERR124256 CNYN02000001 CNYN02000247 10A   10A 97 97 97 1 904732 247 45498 2042 41 3
PT8016 G2Z0B ERS070176 ERR124285 CNZL02000001 CNZL02000178 10A   10A 461 461 461 1 885713 178 48227 1943 44 3
R34-3021 R34-3021 ERS043842 ERR065347 CNPI02000001 CNPI02000197 10A   10A 585 585 585 1 675631 197 62531 1995 41 3
R34-3024 R34-3024 ERS043845 ERR065350 CNPE02000001 CNPE02000138 10A   10A 585 585 585 1 611325 138 62775 2000 43 3
R34-3111 R34-3111 ERS043926 ERR068012 CNWY02000001 CNWY02000163 10A   10A 816 816 816 1 636208 163 81514 1966 45 3
R34-3157 R34-3157 ERS043962 ERR068048 CNXN02000001 CNXN02000214 10A   10A 97 97 97 1 584492 214 62739 2012 38 3
R34-3182 R34-3182 ERS043987 ERR067981 CFID02000001 CFID02000204 10A   10A 816 816 816 1 619369 204 54789 1970 41 3
R34-3184 R34-3184 ERS043989 ERR069683 CNQQ02000001 CNQQ02000274 10A   10A 816 816 816 1 815231 274 34534 1968 31 3
127530 127530 ERS044074 ERR069768 CNTH02000001 CNTH02000239 35F   35F 1953 1953 1953 1 870022 239 44672 1941 31 3
473937 473937 ERS044165 ERR065968 CNPR02000001 CNPR02000272 35F   35F 498 498 498 1 1627916 272 33037 1947 41 2
BR1082 7C7G1 ERS069942 ERR129051 COAX02000001 COAX02000151 35F   35F 498 498 498 1 498464 151 51813 1952 45 3
BR1104 0CNYC ERS069949 ERR129058 CFLT02000001 CFLT02000144 35F   35F 498 498 498 1 467092 144 65135 1958 42 3
CH2084 NIPQY ERS069981 ERR129090 COCF02000001 COCF02000219 35F   35F 498 498 498 1 542021 219 39828 1950 36 3
GL3040 Q7F26 ERS070004 ERR129113 COCX02000001 COCX02000129 35F   35F 498 498 498 1 622745 129 62838 1956 45 3
LE4019 4D0HZ ERS070023 ERR129132 CODD02000001 CODD02000167 35F   35F 498 498 498 1 637013 167 54225 1957 41 3
ND6142 4HUA7 ERS070128 ERR124237 CNYH02000001 CNYH02000190 35F   35F 498 498 498 1 1478239 190 43543 1947 42 3
NP7076 BL0SN ERS070156 ERR124265 CHWY02000001 CHWY02000343 35F   35F 498 498 498 1 755247 343 39673 1971 37 3
PT8011 B50V6 ERS070173 ERR124282 CHWZ02000001 CHWZ02000173 35F   35F 498 498 498 1 990352 173 46695 1945 43 3
PT8076 UVVWA ERS070195 ERR124304 CNZX02000001 CNZX02000184 35F   35F 498 498 498 1 1136340 184 49776 1944 43 3
R34-3112 R34-3112 ERS043927 ERR068013 CHBO02000001 CHBO02000188 35F   35F 498 498 498 1 559483 188 54203 1947 41 3
R34-3118 R34-3118 ERS043932 ERR068018 CNWV02000001 CNWV02000141 35F   35F 498 498 498 1 711343 141 65342 1950 45 3
R34-3120 R34-3120 ERS043934 ERR068020 CNWL02000001 CNWL02000156 35F   35F 498 498 498 1 616726 156 70102 1956 45 3
R34-3178 R34-3178 ERS043983 ERR067977 CNQJ02000001 CNQJ02000235 35F   35F 1916 498 498 1 598635 235 44525 1942 36 3
R34-3191 R34-3191 ERS043996 ERR069690 CNRL02000001 CNRL02000228 35F   35F 498 498 498 1 775377 228 44508 2010 43 3
R34-3213 R34-3213 ERS044018 ERR069712 CNSB02000001 CNSB02000158 35F   35F 498 498 498 1 717789 158 70164 2002 42 3
R34-3216 R34-3216 ERS044021 ERR069715 CNRO02000001 CNRO02000152 35F   35F 498 498 498 1 757343 152 70164 1958 45 3
1996 001996 ERS044038 ERR069732 CNSW02000001 CNSW02000164 6A   6A 1360 1360 1360 1 662636 164 54237 2073 45 3
42397 042397 ERS044046 ERR069740 CGDZ02000001 CGDZ02000162 6A   6A 460 460 460 1 828155 162 63702 2065 45 3
62031 062031 ERS044052 ERR069746 CNSM02000001 CNSM02000213 6A   6A 460 460 460 1 801121 213 45193 2055 42 3
89848 089848 ERS044061 ERR069755 CNTP02000001 CNTP02000211 6A   6A 460 460 460 1 763020 211 57007 2115 46 3
118039 118039 ERS044071 ERR069765 CNTJ02000001 CNTJ02000174 6A   6A 460 460 460 1 847182 174 69053 2061 45 3
135631 135631 ERS044076 ERR069770 CNTG02000001 CNTG02000149 6A   6A   460 460 1 787420 149 60052 2054 45 3
299801 299801 ERS044118 ERR069812 CNUU02000001 CNUU02000137 6A   6A   460 460 1 653068 137 63718 2062 45 3
336850 336850 ERS044129 ERR069823 CIDR02000001 CIDR02000147 6A   6A 460 460 460 1 709263 147 54381 2054 45 3
383739 383739 ERS044141 ERR069835 CNVJ02000001 CNVJ02000145 6A   6A 460 460 460 1 565432 145 69053 2064 45 3
446376 446376 ERS044159 ERR065962 CFLN02000001 CFLN02000324 6A   6A 460 460 460 1 1222840 324 24765 2045 31 3
472699 472699 ERS044164 ERR065967 CNPJ02000001 CNPJ02000188 6A   6A 460 460 460 1 1370061 188 63117 2058 41 3
ND6134 CV6JA ERS070122 ERR124231 CHIS02000001 CHIS02000152 6A   6A 460 460 460 1 824059 152 49835 2050 42 3
R34-3018 R34-3018 ERS043839 ERR065344 CNPA02000001 CNPA02000199 6A   6A 460 460 460 1 406244 199 49705 2056 41 3
R34-3035 R34-3035 ERS043856 ERR065292 CNNG02000001 CNNG02000193 6A   6A 460 460 460 1 732888 193 49705 2058 43 3
R34-3075 R34-3075 ERS043894 ERR065330 CNOT02000001 CNOT02000144 6A   6A 460 460 460 1 476229 144 56775 2061 45 3
R34-3166 R34-3166 ERS043970 ERR067964 CNQX02000001 CNQX02000142 6A   6A 460 460 460 1 560872 142 57073 2039 45 3
R34-3220 R34-3220 ERS044025 ERR069719 CNRU02000001 CNRU02000142 6A   6A 1919 460 460 1 754252 142 57223 2064 45 3
R34-3222 R34-3222 ERS044027 ERR069721 CFQO02000001 CFQO02000146 6A   6A 460 460 460 1 829670 146 69245 2057 45 3
74124 074124 ERS044058 ERR069752 CFJS02000001 CFJS02000112 11A   11D 1913 1913 1913 2 859799 112 73726 1925 44 3
102720 102720 ERS044066 ERR069760 CNTQ02000001 CNTQ02000113 11A   11D   62 62 2 750419 113 70740 1947 45 3
242588 242588 ERS044107 ERR069801 CNUT02000001 CNUT02000118 11A   11D   62 62 2 659925 118 61941 1944 45 3
263137 263137 ERS044110 ERR069804 CNVC02000001 CNVC02000109 11A   11D 62 62 62 2 570680 109 68782 1938 45 3
335574 335574 ERS044128 ERR069822 CNVD02000001 CNVD02000108 11A   11D 62 62 62 2 612642 108 64773 1939 46 3
386329 386329 ERS044143 ERR069837 CNVM02000001 CNVM02000123 11A   11D 1913 1913 1913 2 732342 123 70789 1927 34 3
389109 389109 ERS044145 ERR069839 CNVL02000001 CNVL02000162 11A   11D 62 62 62 2 724678 162 56861 1949 36 3
459747 459747 ERS044161 ERR065964 CNPH02000001 CNPH02000161 11A   11D 62 62 62 2 1718828 161 53562 1985 46 3
BR1039 FP9DM ERS069928 ERR129037 COAM02000001 COAM02000109 11A   11D 62 62 62 2 459396 109 64879 1937 43 3
BR1117 E4NSI ERS069959 ERR129068 COBM02000001 COBM02000109 11A   11D 62 62 62 2 500706 109 65403 1928 46 3
CH2053 DFS2Q ERS069970 ERR129079 COBY02000001 COBY02000151 11A   11D 62 62 62 2 519374 151 49076 1932 41 3
CH2054 K9N0O ERS069971 ERR129080 COCA02000001 COCA02000155 11A   11D 3276 3276 3276 2 535340 155 48753 1925 38 3
CH2091 HWJVH ERS069984 ERR129093 CFKA02000001 CFKA02000119 11A   11D 62 62 62 2 608909 119 59135 1940 45 3
LE4005 R52DJ ERS070018 ERR129127 CFLV02000001 CFLV02000116 11A   11D 62 62 62 2 557016 116 64405 1943 46 3
LE4018 6ZCAZ ERS070022 ERR129131 CODG02000001 CODG02000110 11A   11D 66 62 62 2 698664 110 64405 1939 45 3
LE4040 H3Q9M ERS070028 ERR129137 CHBV02000001 CHBV02000106 11A   11D 3291 62 62 2 530145 106 68768 2001 47 3
LE4050 J1LX6 ERS070030 ERR129139 CFRE02000001 CFRE02000118 11A   11D 62 62 62 2 513346 118 63765 1941 46 3
LE4111 F1G5R ERS070045 ERR129154 CODX02000001 CODX02000093 11A   11D 62 62 62 2 442076 93 77111 1929 46 3
LE4127 F1ODP ERS070050 ERR129159 COEB02000001 COEB02000099 11A   11D 62 62 62 2 449472 99 68793 1986 46 3
MD5070 9ABKR ERS070068 ERR129177 COEO02000001 COEO02000107 11A   11D 62 62 62 2 503807 107 64885 1935 47 3
MD5071 8AH8E ERS070069 ERR129178 COES02000001 COES02000109 11A   11D 62 62 62 2 569828 109 65448 1934 46 3
ND6073 5J97G ERS070101 ERR129210 COFS02000001 COFS02000108 11A   11D 62 62 62 2 816213 108 58324 1924 47 3
ND6085 3YFA4 ERS070102 ERR129211 COFT02000001 COFT02000125 11A   11D 62 62 62 2 551204 125 64405 1994 46 3
ND6103 KYDZM ERS070105 ERR129214 COFW02000001 COFW02000112 11A   11D 62 62 62 2 506942 112 63950 1943 46 3
ND6104 QHXZZ ERS070106 ERR129215 COFV02000001 COFV02000120 11A   11D 62 62 62 2 579315 120 64405 1975 46 3
ND6151 8G3XE ERS070131 ERR124240 CNYB02000001 CNYB02000193 11A   11D 62 62 62 2 1202172 193 41072 1932 31 3
ND6156 T14BF ERS070133 ERR124242 CNYC02000001 CNYC02000112 11A   11D 62 62 62 2 1012160 112 58325 1933 45 3
NP7010 F7IXH ERS070137 ERR124246 CNYK02000001 CNYK02000108 11A   11D 62 62 62 2 1313562 108 59102 1933 47 3
NP7095 RAV85 ERS070159 ERR124268 CFJT02000001 CFJT02000116 11A   11D 62 62 62 2 663964 116 64165 1929 46 3
PT8037 RQ6NH ERS070182 ERR124291 CNZP02000001 CNZP02000109 16F 11 11D 62 62 62 2 825421 109 59058 1933 42 3
PT8053 6CO3C ERS070187 ERR124296 CNZR02000001 CNZR02000140 11A   11D 62 62 62 2 1507531 140 55698 1915 36 3
PT8063 XCJ97 ERS070189 ERR124298 CNZQ02000001 CNZQ02000219 11A   11D 62 62 62 2 1865382 219 49577 1993 42 2
PT8072 28I7H ERS070193 ERR124302 CNZW02000001 CNZW02000104 11A   11D 62 62 62 2 693076 104 70414 1980 47 3
R34-3052 R34-3052 ERS043872 ERR065308 CNNY02000001 CNNY02000119 11A   11D 62 62 62 2 663300 119 68923 1944 45 3
R34-3054 R34-3054 ERS043874 ERR065310 CNOA02000001 CNOA02000104 11A   11D 62 62 62 2 506385 104 72751 1933 46 3
R34-3071 R34-3071 ERS043890 ERR065326 CHBF02000001 CHBF02000117 11A   11D 62 62 62 2 480823 117 71626 1983 46 3
R34-3080 R34-3080 ERS043899 ERR067985 CFRA02000001 CFRA02000116 11A   11D 62 62 62 2 797111 116 64720 1931 46 3
R34-3081 R34-3081 ERS043900 ERR067986 CNWJ02000001 CNWJ02000115 11A   11D 62 62 62 2 781069 115 71626 1938 47 3
R34-3095 R34-3095 ERS043914 ERR068000 CNWE02000001 CNWE02000125 11A   11D 62 62 62 2 673158 125 64778 1959 46 3
R34-3147 R34-3147 ERS043954 ERR068040 CNXD02000001 CNXD02000109 11A   11D 1933 1913 1913 2 563601 109 71626 1933 44 3
R34-3149 R34-3149 ERS043955 ERR068041 CNXF02000001 CNXF02000136 11A   11D 408 408 408 2 580640 136 61830 1973 41 3
R34-3155 R34-3155 ERS043960 ERR068046 CNXL02000001 CNXL02000123 11A   11D 62 62 62 2 700639 123 68173 1948 45 3
R34-3156 R34-3156 ERS043961 ERR068047 CNXJ02000001 CNXJ02000112 11A   11D 62 62 62 2 610537 112 64773 1944 46 3
R34-3170 R34-3170 ERS043974 ERR067968 CNQR02000001 CNQR02000115 11A   11D 62 62 62 2 408141 115 73242 1926 45 3
R34-3203 R34-3203 ERS044008 ERR069702 CNRI02000001 CNRI02000144 11A   11D 62 62 62 2 769725 144 58857 1993 45 3
R34-3208 R34-3208 ERS044013 ERR069707 CNRX02000001 CNRX02000118 11A   11D 62 62 62 2 809261 118 72751 1975 46 3
R34-3214 R34-3214 ERS044019 ERR069713 CNSC02000001 CNSC02000120 11A   11D 62 62 62 2 860741 120 64773 1932 46 3
R34-3228 R34-3228 ERS044033 ERR069727 CNRZ02000001 CNRZ02000108 11A   11D 62 62 62 2 753887 108 68782 1942 45 3
BR1003 SLXY1 ERS069917 ERR129026 CHIT02000001 CHIT02000165 15A   15A 63 63 63 3 579955 165 64610 2081 43 3
BR1086 IB8MW ERS069945 ERR129054 COAZ02000001 COAZ02000130 15A   15A 3282 1914 1914 3 524445 130 68635 1982 45 3
BR1106 W5HGR ERS069951 ERR129060 COBD02000001 COBD02000122 15A   15A 63 63 63 3 512579 122 62192 2051 44 3
BR1109 KRHYA ERS069952 ERR129061 COBE02000001 COBE02000141 15A   15A 63 63 63 3 424418 141 93521 2067 44 3
ND6028 XCB66 ERS070089 ERR129198 COFH02000001 COFH02000122 15A   15A 63 63 63 3 485959 122 69814 1989 45 3
ND6147 UJECB ERS070130 ERR124239 CNYF02000001 CNYF02000176 15A   15A 63 63 63 3 1298010 176 64610 1991 38 3
NP7017 CSI7H ERS070140 ERR124249 CNYO02000001 CNYO02000143 15A   15A 63 63 63 3 951262 143 54713 1980 41 3
NP7105 Z4952 ERS070163 ERR124272 CHIQ02000001 CHIQ02000226 15A   15A 1915 1915 1915 3 1226349 226 56655 2075 42 3
NP7112 XVMDP ERS070165 ERR124274 CFIT02000001 CFIT02000236 15A   15A 1915 1915 1915 3 891236 236 65314 2075 47 3
PT8013 T8K26 ERS070174 ERR124283 CNZJ02000001 CNZJ02000181 15A   15A 63 63 63 3 875403 181 54231 1974 41 3
PT8065 8QTW4 ERS070191 ERR124300 CFIV02000001 CFIV02000259 15A   15A 63 63 63 3 1696803 259 22962 1968 33 3
R34-3036 R34-3036 ERS043857 ERR065293 CNNQ02000001 CNNQ02000133 15A   15A 1914 1914 1914 3 606950 133 65159 1976 45 3
R34-3040 R34-3040 ERS043861 ERR065297 CNNJ02000001 CNNJ02000132 15A   15A 63 63 63 3 647342 132 61778 2001 45 3
R34-3064 R34-3064 ERS043884 ERR065320 CNOI02000001 CNOI02000150 15A   15A 63 63 63 3 430697 150 70075 2068 46 3
R34-3077 R34-3077 ERS043896 ERR065332 CNOR02000001 CNOR02000147 15A   15A 63 63 63 3 495406 147 64598 1995 41 3
R34-3126 R34-3126 ERS043940 ERR068026 CIDQ02000001 CIDQ02000169 15A   15A 63 63 63 3 602486 169 56726 1977 41 3
R34-3130 R34-3130 ERS043942 ERR068028 CHWX02000001 CHWX02000157 15A   15A 63 63 63 3 694582 157 63156 1979 41 3
R34-3138 R34-3138 ERS043946 ERR068032 CNXP02000001 CNXP02000191 15A   15A 63 63 63 3 694702 191 55610 2058 39 3
R34-3150 R34-3150 ERS043956 ERR068042 CNXE02000001 CNXE02000166   15 15A   1914 1914 3 654017 166 56424 1968 43 3
R34-3158 R34-3158 ERS043963 ERR068049 CNXM02000001 CNXM02000194 15A   15A 63 63 63 3 618935 194 57605 2065 42 3
R34-3179 R34-3179 ERS043984 ERR067978 CNQK02000001 CNQK02000173 15A   15A 1915 1915 1915 3 673246 173 56726 2030 42 3
R34-3225 R34-3225 ERS044030 ERR069724 CNRS02000001 CNRS02000136 15A   15A 63 63 63 3 742785 136 65204 1995 43 3
R34-3226 R34-3226 ERS044031 ERR069725 CHBI02000001 CHBI02000139 15A   15A 63 63 63 3 733637 139 63156 1994 43 3
ND6117 HB7S5 ERS070112 ERR124221 CFIR02000001 CFIR02000119 19A   19A 63 63 63 3 850674 119 58974 2009 45 3
R34-3199 R34-3199 ERS044004 ERR069698 CNRG02000001 CNRG02000154 19A   19A 63 63 63 3 802951 154 54701 2053 45 3
35100 035100 ERS044044 ERR069738 CNSI02000001 CNSI02000135 22F   22F 1901 1901 1901 4 742235 135 56595 2053 46 3
120581 120581 ERS044073 ERR069767 CNTI02000001 CNTI02000163 22F   22F 433 433 433 4 804432 163 46699 1995 43 3
153408 153408 ERS044084 ERR069778 CNUK02000001 CNUK02000130 22F   22F 433 433 433 4 820300 130 55492 2039 48 3
163286 163286 ERS044089 ERR069783 CNUN02000001 CNUN02000129 22F   22F 433 433 433 4 809024 129 53587 1997 47 3
385385 385385 ERS044142 ERR069836 CNVI02000001 CNVI02000134 22F   22F 433 433 433 4 727420 134 56595 2049 48 3
CH2108 RWZJE ERS069987 ERR129096 COCO02000001 COCO02000121 22F   22F 433 433 433 4 553476 121 57139 1992 47 3
LE4118 FBPNS ERS070047 ERR129156 CODZ02000001 CODZ02000138 22F   22F 433 433 433 4 496383 138 56232 1990 43 4
ND6047 120T7 ERS070095 ERR129204 COFQ02000001 COFQ02000135 22F   22F 433 433 433 4 266801 135 56753 2004 44 3
NP7013 FJZZW ERS070139 ERR124248 CNYM02000001 CNYM02000145 22F   22F 433 433 433 4 997588 145 52943 1992 42 3
NP7062 Q3H1U ERS070151 ERR124260 CFLO02000001 CFLO02000165 22F   22F 1901 1901 1901 4 708360 165 42558 2049 46 3
PT8085 DQKYE ERS070198 ERR124307 COAA02000001 COAA02000150 22F   22F 433 433 433 4 1155165 150 44870 1994 47 2
PT8116 I0UZQ ERS070209 ERR124318 COAF02000001 COAF02000109 22F   22F 433 433 433 4 428840 109 50671 1965 45 3
R34-3012 R34-3012 ERS043833 ERR065338 CNOY02000001 CNOY02000144 22F   22F   1337 1337 4 574493 144 61942 2042 42 3
R34-3017 R34-3017 ERS043838 ERR065343 CNPG02000001 CNPG02000134 22F   22F 433 433 433 4 530776 134 61715 2018 45 3
R34-3082 R34-3082 ERS043901 ERR067987 CNWI02000001 CNWI02000136 22F   22F 1337 1337 1337 4 692808 136 59103 2044 46 3
R34-3090 R34-3090 ERS043909 ERR067995 CNVZ02000001 CNVZ02000151 22F   22F 1337 1337 1337 4 687236 151 56964 2047 46 3
R34-3091 R34-3091 ERS043910 ERR067996 CNWA02000001 CNWA02000125 22F   22F 433 433 433 4 678272 125 59334 1967 45 3
R34-3143 R34-3143 ERS043951 ERR068037 CNXQ02000001 CNXQ02000130 22F   22F 433 433 433 4 726946 130 58849 1966 45 3
R34-3167 R34-3167 ERS043971 ERR067965 CFJR02000001 CFJR02000119 22F   22F 433 433 433 4 578393 119 56746 1995 46 3
R34-3187 R34-3187 ERS043992 ERR069686 CNRN02000001 CNRN02000128 22F   22F 433 433 433 4 807317 128 58409 1991 46 3
R34-3189 R34-3189 ERS043994 ERR069688 CNRA02000001 CNRA02000120 22F   22F 433 433 433 4 735648 120 64385 1978 42 3
BR1085 VPPXV ERS069944 ERR129053 CIKC02000001 CIKC02000133 11A   11A 156 156 156 5 539305 133 57158 2026 48 3
LE4073 3FBXJ ERS070035 ERR129144 CHBX02000001 CHBX02000258 15B/C   15C 162 162 162 5 665190 258 50011 2146 44 2
MD5103 NJ41D ERS070073 ERR129182 COEU02000001 COEU02000189 15B/C   15B 3275 3275 3275 5 550506 189 54884 2087 50 3
ND6019 5MZ1D ERS070087 ERR129196 COFN02000001 COFN02000332 15B/C   15C 162 162 162 5 604860 332 54585 2174 44 3
R34-3109 R34-3109 ERS043924 ERR068010 CHXA02000001 CHXA02000284   15 15B   162 162 5 527501 284 49960 2078 46 3
GL3021 F8P25 ERS069996 ERR129105 COCT02000001 COCT02000141 19A   19A 1925 1925 1925 5 544855 141 73234 2030 48 3
ND6017 XQR0B ERS070086 ERR129195 COFF02000001 COFF02000150 19A   19A 1925 1925 1925 5 575890 150 59020 2035 48 3
R34-3093 R34-3093 ERS043912 ERR067998 CNWC02000001 CNWC02000171 19A   19A 1925 1925 1925 5 630576 171 84759 1974 45 3
R34-3215 R34-3215 ERS044020 ERR069714 CNSH02000001 CNSH02000166 19A   19A 1925 1925 1925 5 756771 166 80712 2036 44 3
234323 234323 ERS044105 ERR069799 CFQE02000001 CFQE02000139 9A   9V 156 156 156 5 618848 139 79769 2031 49 3
312942 312942 ERS044124 ERR069818 CNVA02000001 CNVA02000142 9A   9V 156 156 156 5 593918 142 76463 2023 48 3
422264 422264 ERS084155 ERR124320 COAH02000001 COAH02000221 9A   9A 156 156 156 5 458625 221 58853 2104 51 3
R34-3016 R34-3016 ERS043837 ERR065342 CHWT02000001 CHWT02000142 9A   9V 156 156 156 5 399840 142 61379 2039 48 3
CH2029 STPDE ERS069968 ERR129077 COBW02000001 COBW02000214 9V   9V 162 162 162 5 527198 214 46466 2083 48 3
R34-3045 R34-3045 ERS043865 ERR065301 CNNT02000001 CNNT02000129 9V   9V 156 156 156 5 525405 129 80077 2028 49 3
PT8019 HR2T3 ERS070177 ERR124286 CHBT02000001 CHBT02000131 15B/C   15B 199 3280 3280 6 1196963 131 77326 2029 46 3
PT8025 0FQ8K ERS070179 ERR124288 CIDT02000001 CIDT02000148 15B/C   15C 3280 3280 3280 6 1052080 148 75361 2024 46 3
PT8044 H8YKW ERS070185 ERR124294 CFQZ02000001 CFQZ02000133 15B/C   17F 3280 3280 3280 6 822160 133 73299 2022 46 3
PT8054 01ZWM ERS070188 ERR124297 CNZS02000001 CNZS02000178 15B/C   15C 3280 3280 3280 6 1115345 178 63357 2020 39 3
PT8120 BJF60 ERS070210 ERR124319 COAI02000001 COAI02000130 15B/C   15C 3280 3280 3280 6 405752 130 77366 2038 46 3
BR1055 0GB6K ERS069933 ERR129042 CHXE02000001 CHXE02000137 23A   23A 338 338 338 6 636440 137 68852 1992 44 3
GL3050 OSEY6 ERS070008 ERR129117 COCY02000001 COCY02000132 23A   23A 338 338 338 6 515889 132 73610 2003 44 3
LE4119 DNB90 ERS070048 ERR129157 CODW02000001 CODW02000128 23A   23A 338 338 338 6 408017 128 74023 2057 45 3
MD5029 5XCD5 ERS070058 ERR129167 COEI02000001 COEI02000186 23A   23A 338 338 338 6 525551 186 59110 2106 35 3
MD5068 TV66E ERS070067 ERR129176 COER02000001 COER02000154 23A   23A 338 338 338 6 502574 154 63937 1998 42 3
ND6100 UNUOJ ERS070104 ERR129213 COFR02000001 COFR02000152 23A   23A 338 338 338 6 552262 152 70236 2054 42 3
ND6140 28PCJ ERS070126 ERR124235 CNXX02000001 CNXX02000140 23A   23A 338 338 338 6 855744 140 63800 2014 44 3
PT8086 A12WS ERS070199 ERR124308 CFRS02000001 CFRS02000138 23A   23A 338 338 338 6 1008797 138 68852 1990 45 3
R34-3047 R34-3047 ERS043867 ERR065303 CFQX02000001 CFQX02000142 23A   23A 338 338 338 6 580649 142 69404 1995 45 3
R34-3123 R34-3123 ERS043937 ERR068023 CFRD02000001 CFRD02000128 23A   23A 338 338 338 6 611026 128 69536 1986 43 3
R34-3209 R34-3209 ERS044014 ERR069708 CFQF02000001 CFQF02000139 23A   23A 338 338 338 6 855583 139 70958 1997 45 3
BR1058 O3452 ERS069934 ERR129043 COAR02000001 COAR02000161 23B   23B 1373 1373 1373 6 550577 161 69549 2107 41 3
ND6124 1LQKE ERS070118 ERR124227 CFIS02000001 CFIS02000182 23B   23B 1373 1373 1373 6 1567202 182 60867 2104 43 3
R34-3136 R34-3136 ERS043944 ERR068030 CNXK02000001 CNXK02000135 23A   23F 342 342 342 6 718246 135 75703 2060 48 3
153438 153438 ERS044085 ERR069779 CNUE02000001 CNUE02000178 6B   6A 1827 1827 1827 6 746362 178 58069 2017 38 3
174702 174702 ERS044092 ERR069786 CNTT02000001 CNTT02000182 6B   6A 639 639 639 6 839574 182 66518 1978 40 3
223832 223832 ERS044104 ERR069798 CNUJ02000001 CNUJ02000161   6 6A   138 138 6 709724 161 65138 2012 46 3
481643 481643 ERS044166 ERR065969 CFQD02000001 CFQD02000221 6B   6A 138 138 138 6 1633294 221 42901 2007 41 3
487827 487827 ERS044168 ERR065971 CNPS02000001 CNPS02000180 6B   6A 138 138 138 6 1428334 180 60779 2000 44 3
MD5037 UB6XH ERS070063 ERR129172 CIIN02000001 CIIN02000160 6B   6A 138 138 138 6 486943 160 64834 2061 45 3
R34-3186 R34-3186 ERS043991 ERR069685 CNRF02000001 CNRF02000174 6B   6A 138 138 138 6 867942 174 59130 2082 45 3
R34-3194 R34-3194 ERS043999 ERR069693 CNRE02000001 CNRE02000165 6B   6A 176 5006 5006 6 789106 165 69823 2053 45 3
MD5030 1TR6C ERS070059 ERR129168 COEH02000001 COEH02000199 6C   6C 138 138 138 6 648781 199 64315 2077 44 2
BR1116 7RCF0 ERS069958 ERR129067 COBP02000001 COBP02000155 7F   7A 191 191 191 7 645774 155 48777 1922 45 3
CH2008 UFG1A ERS069964 ERR129073 COBT02000001 COBT02000140 7F   7F 191 191 191 7 513954 140 57733 1929 45 3
ND6120 RJBFJ ERS070115 ERR124224 CIED02000001 CIED02000153 7F   7A 191 191 191 7 1562436 153 48777 1923 45 3
ND6130 B4R32 ERS070119 ERR124228 CFJV02000001 CFJV02000308 7F   7F 191 191 191 7 973684 308 18795 1912 32 1
ND6158 3BDEM ERS070134 ERR124243 CFQV02000001 CFQV02000149 7F   7F 191 191 191 7 809421 149 53335 1933 45 3
NP7118 Y89K0 ERS070167 ERR124276 CNZE02000001 CNZE02000145 7F   7F 191 191 191 7 716414 145 53335 1928 45 3
PT8039 D8RPY ERS070183 ERR124292 CNZU02000001 CNZU02000156 7F   7A 191 191 191 7 968790 156 46612 1922 45 3
PT8094 55NO4 ERS070203 ERR124312 COAB02000001 COAB02000178 7F   7A 191 191 191 7 1303735 178 45214 1925 46 3
R34-3163 R34-3163 ERS043967 ERR067961 CNQM02000001 CNQM02000161 7F   7A 191 191 191 7 537018 161 48765 1920 45 3
R34-3219 R34-3219 ERS044024 ERR069718 CNRQ02000001 CNRQ02000159 7F   7A 191 191 191 7 812469 159 47051 1923 46 3
72782 072782 ERS044057 ERR069751 CNSY02000001 CNSY02000137 15B/C   15C 199 199 199 8 812550 137 69458 1974 45 3
103453 103453 ERS044067 ERR069761 CNSX02000001 CNSX02000168 15B/C   15C 1938 1938 1938 8 696916 168 73491 2024 44 3
146066 146066 ERS044080 ERR069774 CNTK02000001 CNTK02000166 15B/C   15C 199 199 199 8 871621 166 68997 1972 45 2
217475 217475 ERS044101 ERR069795 CHIP02000001 CHIP02000177 15B/C   15C 199 199 199 8 687293 177 67617 1982 38 3
362412 362412 ERS044134 ERR069828 CNVV02000001 CNVV02000225   15 15B   199 199 8 792208 225 36881 1999 35 3
365152 365152 ERS044135 ERR069829 CNVU02000001 CNVU02000166 15B/C   15C   199 199 8 642192 166 82074 1967 45 3
372297 372297 ERS044139 ERR069833 CFLQ02000001 CFLQ02000144 15B/C   15B 199 199 199 8 586284 144 82128 1982 41 3
483391 483391 ERS044167 ERR065970 CNPQ02000001 CNPQ02000152 15B/C   15C 199 199 199 8 1486818 152 66331 1963 45 3
492706 492706 ERS044169 ERR065972 CNPW02000001 CNPW02000175 15B/C   15C 199 199 199 8 1330422 175 54120 1963 41 3
509365 509365 ERS044172 ERR065975 CNPV02000001 CNPV02000205 15B/C   15C 199 199 199 8 1561835 205 58597 2021 41 3
BR1022-2 DH8X5-2 ERS069923 ERR129032 COBQ02000001 COBQ02000160 15B/C   15C 199 199 199 8 595975 160 62867 2021 45 3
BR1023 K51SW ERS069924 ERR129033 COBS02000001 COBS02000213 15B/C   15C 199 199 199 8 613795 213 69142 2032 42 3
BR1025 PXB8V ERS069925 ERR129034 CFRH02000001 CFRH02000248 15B/C   15C 3280 199 199 8 483133 248 50861 2018 41 3
BR1068 TG9A8 ERS069937 ERR129046 COAU02000001 COAU02000182 15B/C   15C 199 199 199 8 529229 182 69469 2031 44 3
CH2060 FQPLS ERS069972 ERR129081 COCC02000001 COCC02000141 15B/C   15C 199 199 199 8 594249 141 75646 1975 44 3
CH2085 WQ9GV ERS069982 ERR129091 COCH02000001 COCH02000158 15B/C   15C 199 199 199 8 598594 158 64845 1958 41 3
GL3071 IRE4I ERS070012 ERR129121 CODB02000001 CODB02000154 15B/C   15C 199 199 199 8 523593 154 82175 2032 42 1
GL3080 P4GIC ERS070016 ERR129125 CFRI02000001 CFRI02000164 15B/C   15C 199 199 199 8 556405 164 81950 2025 43 3
LE4011 EH2WN ERS070020 ERR129129 COEJ02000001 COEJ02000141 15B/C   15B 199 199 199 8 514322 141 82185 1981 44 3
LE4092 XZHZP ERS070040 ERR129149 CODR02000001 CODR02000160 15B/C   15C 199 199 199 8 557647 160 62958 2019 44 3
MD5045 AYFPY ERS070065 ERR129174 COEQ02000001 COEQ02000183 15B/C   15C 3286 199 199 8 568263 183 69028 2008 41 3
MD5052 IMAPY ERS070066 ERR129175 CHXK02000001 CHXK02000147 15B/C   15C 199 199 199 8 562835 147 84660 2024 45 3
MD5110 QLCSW ERS070077 ERR129186 CHXG02000001 CHXG02000146 15B/C   15B 199 199 199 8 455340 146 82265 1974 43 3
ND6006 5QBRP ERS070081 ERR129190 COEY02000001 COEY02000145 15B/C   15B 199 199 199 8 536559 145 68872 1961 45 3
ND6121 PN3IH ERS070116 ERR124225 CNYY02000001 CNYY02000184 15B/C   15C 199 199 199 8 1254362 184 39433 1977 41 3
ND6132 ESBKM ERS070120 ERR124229 CNXU02000001 CNXU02000160 15B/C   15C 199 199 199 8 765233 160 82188 2027 41 3
NP7066 PCKMO ERS070154 ERR124263 CNYV02000001 CNYV02000245 15B/C   15C 199 199 199 8 1350126 245 34161 1974 39 3
NP7081 LJ6JL ERS070158 ERR124267 CNYZ02000001 CNYZ02000130 15B/C   15B 199 199 199 8 791051 130 67643 1966 42 3
PT8098 IZCEG ERS070204 ERR124313 COAD02000001 COAD02000140 15B/C   15C 199 199 199 8 500975 140 82185 2018 45 3
R34-3011 R34-3011 ERS043832 ERR065337 CFJQ02000001 CFJQ02000179   15 15B   199 199 8 656273 179 68606 2024 45 3
R34-3014 R34-3014 ERS043835 ERR065340 CNPP02000001 CNPP02000150 15B/C   15B 199 199 199 8 682945 150 68971 1966 45 3
R34-3030 R34-3030 ERS043851 ERR065287 CNNI02000001 CNNI02000162 15B/C   15C 199 199 199 8 651692 162 58594 2018 45 3
R34-3031 R34-3031 ERS043852 ERR065288 CNNP02000001 CNNP02000165 15B/C   15C 199 199 199 8 611548 165 71959 1981 46 3
R34-3034 R34-3034 ERS043855 ERR065291 CNNH02000001 CNNH02000177 15B/C   15C 199 199 199 8 594947 177 58598 2037 46 3
R34-3140 R34-3140 ERS043948 ERR068034 CFJX02000001 CFJX02000182 15B/C   15B 199 199 199 8 650075 182 59385 1970 43 3
R34-3144 R34-3144 ERS043952 ERR068038 CNXB02000001 CNXB02000150 15B/C   15C 199 199 199 8 648996 150 76332 1971 45 3
R34-3153 R34-3153 ERS043958 ERR068044 CNXI02000001 CNXI02000162 15B/C   15C 199 199 199 8 634121 162 68873 1981 43 3
R34-3154 R34-3154 ERS043959 ERR068045 CNXH02000001 CNXH02000167 15B/C   15B 199 199 199 8 597608 167 69008 2007 41 3
R34-3159 R34-3159 ERS043964 ERR068050 CNXO02000001 CNXO02000286 15B/C   15C 1932 1932 1932 8 602138 286 32502 1957 31 3
R34-3160 R34-3160 ERS043966 ERR067960 CNQB02000001 CNQB02000159 15B/C   15C 199 199 199 8 655277 159 66332 1984 45 3
R34-3175 R34-3175 ERS043980 ERR067974 CNQF02000001 CNQF02000189 15B/C   15C 199 199 199 8 686799 189 69088 2027 42 3
R34-3188 R34-3188 ERS043993 ERR069687 CNRM02000001 CNRM02000152 15B/C   15C 199 199 199 8 784459 152 86418 2023 45 3
R34-3192 R34-3192 ERS043997 ERR069691 CNRK02000001 CNRK02000163 15B/C   15C 199 199 199 8 825017 163 56307 1988 45 3
R34-3205 R34-3205 ERS044010 ERR069704 CNQU02000001 CNQU02000156 15B/C   15C 199 199 199 8 785495 156 76350 1956 45 3
R34-3206 R34-3206 ERS044011 ERR069705 CNRB02000001 CNRB02000157 15B/C   15C 199 199 199 8 728920 157 82190 1951 45 3
R34-3212 R34-3212 ERS044017 ERR069711 CHBJ02000001 CHBJ02000159 15B/C   15C 199 199 199 8 640200 159 68046 1984 45 3
ND6053 8PY5X ERS070098 ERR129207 COFO02000001 COFO02000165 15F   15C 199 199 199 8 656525 165 85258 2016 44 3
ND6116 M6AJV ERS070111 ERR124220 CNYI02000001 CNYI02000172 15F   15C 199 199 199 8 876332 172 58608 2011 38 3
NP7022 WWC1T ERS070142 ERR124251 CFIU02000001 CFIU02000172 15F   15C 199 199 199 8 936218 172 51800 2010 44 3
R34-3063 R34-3063 ERS043883 ERR065319 CNOK02000001 CNOK02000182 15F   15B 199 199 199 8 486883 182 68971 2057 46 3
57144 057144 ERS044051 ERR069745 CNSK02000001 CNSK02000195 19A   19A 199 199 199 8 750356 195 64801 2078 46 3
80104 080104 ERS044059 ERR069753 CNTC02000001 CNTC02000175 19A   19A 199 199 199 8 836317 175 68967 1968 47 3
135771 135771 ERS044077 ERR069771 CNTN02000001 CNTN02000165 19A   19A 199 199 199 8 768304 165 69133 1967 47 3
148056 148056 ERS044082 ERR069776 CNTZ02000001 CNTZ02000166 19A   19A 1936 1936 1936 8 857827 166 69132 2032 46 0
160449 160449 ERS044087 ERR069781 CNUG02000001 CNUG02000149 19A   19A 199 199 199 8 668543 149 69133 1964 47 3
210162 210162 ERS044100 ERR069794 CNUD02000001 CNUD02000187 19A   19A 1936 1936 1936 8 653397 187 68508 2021 39 3
218186 218186 ERS044102 ERR069796 CNUH02000001 CNUH02000160 19A   19A 199 199 199 8 727078 160 60163 1960 47 3
266035 266035 ERS044111 ERR069805 CIDV02000001 CIDV02000165 19A   19A 199 199 199 8 641832 165 68508 2019 48 3
312034 312034 ERS044123 ERR069817 CNUS02000001 CNUS02000152 19F 19A 19A 199 199 199 8 589224 152 68927 2021 47 3
366293 366293 ERS044137 ERR069831 CNVH02000001 CNVH02000146 19A   19A 199 199 199 8 772939 146 68983 1965 46 3
BR1000 6EO69 ERS069916 ERR129025 COAK02000001 COAK02000239 19A   19A 1756 1756 1756 8 418952 239 39414 2014 36 3
BR1105 RZPNF ERS069950 ERR129059 COBC02000001 COBC02000146 19A   19A 199 199 199 8 518047 146 58735 1970 46 3
BR1112 6UUPJ ERS069954 ERR129063 CHXL02000001 CHXL02000154 19A   19A 199 199 199 8 479533 154 60175 1963 44 3
CH2007 BHCQB ERS069963 ERR129072 COBL02000001 COBL02000121 19A   19A 199 199 199 8 614992 121 68308 1963 47 3
GL3029 RCBGR ERS069999 ERR129108 CFKC02000001 CFKC02000173 19A   19A 199 199 199 8 555058 173 68189 2048 45 3
GL3036 WNYEL ERS070003 ERR129112 COCW02000001 COCW02000152 19A   19A 199 199 199 8 494197 152 69143 1967 45 3
GL3060 WLTN8 ERS070009 ERR129118 CODA02000001 CODA02000164 19A   19A 2344 2344 2344 8 639781 164 61840 1979 45 3
GL3066 RL36E ERS070011 ERR129120 CODE02000001 CODE02000196 19A   19A 199 199 199 8 662815 196 60175 2114 48 2
GL3077 REQGJ ERS070014 ERR129123 CODU02000001 CODU02000166 19A   19A 10013 199 8563 8 543895 166 69029 1969 39 2
LE4055 LOOXY ERS070031 ERR129140 CFIW02000001 CFIW02000168 19A   19A 199 199 199 8 558101 168 56962 2020 47 3
LE4059 FUOYQ ERS070033 ERR129142 CODK02000001 CODK02000195 19A   19A 199 199 199 8 461991 195 60175 2054 42 3
LE4106 Z60YP ERS070044 ERR129153 CODS02000001 CODS02000150 19A   19A 199 199 199 8 521607 150 69236 1969 47 3
LE4142 DPGZI ERS070051 ERR129160 COED02000001 COED02000149 19A   19A 199 199 199 8 475325 149 69186 2030 47 3
MD5033 YQUAQ ERS070061 ERR129170 CFIF02000001 CFIF02000135 19A   19A 199 199 199 8 465051 135 73638 1962 45 3
MD5104 IZQWI ERS070074 ERR129183 COEW02000001 COEW02000144 19A   19A 667 667 667 8 415931 144 68975 1962 44 3
ND6022 1V4ME ERS070088 ERR129197 COFJ02000001 COFJ02000198 19A   19A 199 199 199 8 579270 198 46314 1969 38 3
ND6064 VQC7K ERS070100 ERR129209 CHXI02000001 CHXI02000144 19A   19A 199 199 199 8 582921 144 66146 1970 45 3
ND6139 KMMCT ERS070125 ERR124234 CNXW02000001 CNXW02000168 19A   19A 199 199 199 8 877923 168 50251 1954 38 3
NP7100 6JSY8 ERS070160 ERR124269 CNZC02000001 CNZC02000221 19A   19A 3279 199 199 8 772668 221 41089 1951 41 3
NP7101 0OH1I ERS070161 ERR124270 CNZB02000001 CNZB02000184 19A   19A 876 876 876 8 799999 184 69191 2046 42 3
PT8004 LIW28 ERS070171 ERR124280 CNZG02000001 CNZG02000171 19A   19A 667 667 667 8 905301 171 59025 2013 40 3
PT8009 6FXSQ ERS070172 ERR124281 CNZH02000001 CNZH02000180 19A   19A 667 667 667 8 1063222 180 52132 1956 41 3
PT8014 2EAKR ERS070175 ERR124284 CNZK02000001 CNZK02000172 19A   19A 199 199 199 8 930530 172 56961 1962 36 3
PT8052 RMEOX ERS070186 ERR124295 CFLR02000001 CFLR02000173 19A   19A 1673 1673 1673 8 887694 173 50251 1966 41 3
PT8106 ATBLM ERS070206 ERR124315 CFIE02000001 CFIE02000159 19A   19A 199 199 199 8 455535 159 49130 1985 45 3
R34-3049 R34-3049 ERS043869 ERR065305 CNNU02000001 CNNU02000159 19A   19A 667 667 667 8 671850 159 68918 1966 45 3
R34-3061 R34-3061 ERS043881 ERR065317 CFQR02000001 CFQR02000182 19A   19A 667 667 667 8 499173 182 57189 1947 45 3
R34-3068 R34-3068 ERS043887 ERR065323 CNON02000001 CNON02000156 19A   19A 199 199 199 8 544702 156 69027 1971 47 3
R34-3073 R34-3073 ERS043892 ERR065328 CNOP02000001 CNOP02000173 19A   19A 199 199 199 8 456111 173 69027 2027 43 3
R34-3079 R34-3079 ERS043898 ERR067984 CNWD02000001 CNWD02000161 19A   19A 199 199 199 8 738681 161 68981 1968 47 3
R34-3103 R34-3103 ERS043922 ERR068008 CNWS02000001 CNWS02000207 19A   19A 199 199 199 8 741583 207 56953 2074 45 3
R34-3110 R34-3110 ERS043925 ERR068011 CHWW02000001 CHWW02000157 19A   19A 1935 1935 1935 8 531414 157 68973 1949 46 3
R34-3116 R34-3116 ERS043931 ERR068017 CIIL02000001 CIIL02000166 19A   19A 199 199 199 8 731041 166 69027 1972 46 3
R34-3124 R34-3124 ERS043938 ERR068024 CNWM02000001 CNWM02000171 19A   19A 199 199 199 8 646107 171 60163 1957 47 3
R34-3185 R34-3185 ERS043990 ERR069684 CNQV02000001 CNQV02000177 19A   19A 199 199 199 8 791227 177 69027 1957 44 3
R34-3197 R34-3197 ERS044002 ERR069696 CNRD02000001 CNRD02000164 19A   19A 199 199 199 8 735713 164 71041 1973 46 3
R34-3218 R34-3218 ERS044023 ERR069717 CIDX02000001 CIDX02000166 19A   19A 199 199 199 8 929092 166 69062 1962 47 2
95344 095344 ERS044064 ERR069758 CHXD02000001 CHXD02000176 7   7C   199 199 8 791422 176 64700 1971 33 3
276430 276430 ERS044113 ERR069807 CNUF02000001 CNUF02000133 18C   18C   SLV42 6712 9 555035 133 86170 2009 45 3
154389 154389 ERS044086 ERR069780 CIIJ02000001 CIIJ02000130 23A   23A 436 436 436 9 719341 130 74001 2038 45 3
354557 354557 ERS044132 ERR069826 CNVQ02000001 CNVQ02000141 23A   23A 436 436 436 9 721943 141 84476 2032 45 3
BR1090 ON38S ERS069946 ERR129055 CHBW02000001 CHBW02000122 23A   23A 42 42 42 9 547753 122 80068 2005 44 3
BR1114 5ZS1X ERS069956 ERR129065 COBI02000001 COBI02000125 23A   23A 3287 439 439 9 529650 125 84607 2024 44 3
CH2026 9T42W ERS069967 ERR129076 COBU02000001 COBU02000163 23A   23A 438 438 438 9 563573 163 79466 1981 40 3
CH2061 TEF7S ERS069973 ERR129082 COBX02000001 COBX02000126 23A   23A 190 190 190 9 602853 126 79182 2013 43 3
CH2116 2AHBE ERS069989 ERR129098 COCN02000001 COCN02000165 23A   23A 438 438 438 9 547181 165 72647 1995 40 3
GL3023 J9GMM ERS069997 ERR129106 CFRC02000001 CFRC02000134 23A   23A 1839 1839 1839 9 628593 134 74397 2048 45 3
GL3049 YHPD0 ERS070007 ERR129116 CFLU02000001 CFLU02000113 23A   23A 3285 1839 1839 9 566727 113 80565 2003 46 3
LE4020 CJISL ERS070024 ERR129133 CODI02000001 CODI02000132 23A   23A 42 42 42 9 483668 132 74087 2008 43 3
LE4058 LIT4Q ERS070032 ERR129141 CODP02000001 CODP02000123 23A   23A 1839 1839 1839 9 513277 123 84488 2050 46 3
LE4081 ZXPKH ERS070037 ERR129146 CODO02000001 CODO02000122 23F   23A 42 42 42 9 545528 122 89884 2006 45 3
ND6051 94ORH ERS070096 ERR129205 COFL02000001 COFL02000120 23A   23A 42 42 42 9 717150 120 95546 2012 45 3
PT8021 Y0160 ERS070178 ERR124287 CNZN02000001 CNZN02000343 23A   23A 3289 6712 6712 9 1057321 343 18594 1978 26 3
R34-3050 R34-3050 ERS043870 ERR065306 CNNW02000001 CNNW02000134 23A   23A 1949 6712 6712 9 660213 134 89286 2010 46 3
R34-3051 R34-3051 ERS043871 ERR065307 CNNX02000001 CNNX02000132 23A   23A 438 438 438 9 593281 132 90651 2007 45 3
R34-3055 R34-3055 ERS043875 ERR065311 CNOB02000001 CNOB02000116 23A   23A 1839 1839 1839 9 465525 116 97954 2011 47 3
R34-3057 R34-3057 ERS043877 ERR065313 CNOC02000001 CNOC02000132 23A   23A 438 438 438 9 482946 132 79454 1997 46 3
R34-3067 R34-3067 ERS043886 ERR065322 CNOM02000001 CNOM02000120 23A   23A 1839 1839 1839 9 549576 120 97720 2002 47 3
R34-3127 R34-3127 ERS043941 ERR068027 CNWX02000001 CNWX02000135 23A   23A 1839 1839 1839 9 481401 135 84477 1998 43 3
R34-3174 R34-3174 ERS043979 ERR067973 CNQG02000001 CNQG02000122 23A   23A 1839 1839 1839 9 661844 122 86185 1996 47 3
R34-3177 R34-3177 ERS043982 ERR067976 CNQI02000001 CNQI02000133 23A   23A 42 42 42 9 611198 133 81077 2011 47 3
248734 248734 ERS044108 ERR069802 CNUZ02000001 CNUZ02000132 23B   23B 439 439 439 9 626347 132 73552 1989 45 3
BR1014 FOR95 ERS069920 ERR129029 COBO02000001 COBO02000110 23B   23B 36 36 36 9 525350 110 97610 2016 42 3
BR1084 QRPPW ERS069943 ERR129052 COAY02000001 COAY02000102 23B   23B 36 36 36 9 606062 102 80281 2014 44 3
CH2124 C3W41 ERS069991 ERR129100 COCM02000001 COCM02000195 23B   23B 1847 1847 1847 9 535613 195 58297 2089 41 3
GL3024 DYJGL ERS069998 ERR129107 CFJZ02000001 CFJZ02000126 23B   23B 439 439 439 9 514306 126 73569 2009 44 3
GL3075 WCULS ERS070013 ERR129122 CFLP02000001 CFLP02000149 23B   23B 439 439 439 9 602400 149 63696 2052 45 3
LE4083 3MB4Z ERS070038 ERR129147 CHXF02000001 CHXF02000126 23B   23B 439 439 439 9 473096 126 81334 2034 43 3
LE4117 WYMQI ERS070046 ERR129155 CODY02000001 CODY02000154 23B   23B 439 439 439 9 511369 154 58632 2039 39 3
LE4150 BDGWM ERS070052 ERR129161 COEE02000001 COEE02000145 23B   23B 1847 1847 1847 9 435042 145 73566 2094 45 3
MD5035 8A4VR ERS070062 ERR129171 COEN02000001 COEN02000136 23B   23B 36 36 36 9 515661 136 84483 2071 45 3
ND6008 DP07V ERS070083 ERR129192 COFB02000001 COFB02000137 23B   23B 3281 1448 1448 9 581471 137 74061 2041 34 3
ND6059 7LLCL ERS070099 ERR129208 CFRL02000001 CFRL02000152 23B   23B 1847 1847 1847 9 628205 152 58264 2099 44 3
ND6115 T7IZ1 ERS070110 ERR124219 CHIR02000001 CHIR02000127 23B   23B 36 36 36 9 981592 127 74457 2018 44 3
ND6144 LFB15 ERS070129 ERR124238 CFKB02000001 CFKB02000139 23B   23B 439 439 439 9 1203429 139 70438 2009 44 3
NP7006 04WB3 ERS070136 ERR124245 CNYE02000001 CNYE02000256 23B   23B 1847 1847 1847 9 1055259 256 48628 2087 42 3
R34-3019 R34-3019 ERS043840 ERR065345 CNPB02000001 CNPB02000176 23B   23B 439 439 439 9 532656 176 63728 2042 47 3
R34-3020 R34-3020 ERS043841 ERR065346 CNOS02000001 CNOS02000156 23B   23B 436 439 439 9 528955 156 65013 2045 46 3
R34-3022 R34-3022 ERS043843 ERR065348 CNOU02000001 CNOU02000171 23B   23B 1847 1847 1847 9 467280 171 59893 2091 42 3
R34-3114 R34-3114 ERS043929 ERR068015 CIKP02000001 CIKP02000140 23B   23B 439 439 439 9 499449 140 58852 2025 46 3
R34-3169 R34-3169 ERS043973 ERR067967 CHBH02000001 CHBH02000154 23B   23B 439 439 439 9 634450 154 57848 2015 45 3
R34-3195 R34-3195 ERS044000 ERR069694 CNRC02000001 CNRC02000153 23B   23B 439 439 439 9 800201 153 77317 2059 45 3
25310 025310 ERS044042 ERR069736 CNTE02000001 CNTE02000118 23F   23F 36 36 36 9 772735 118 94139 2034 45 3
49470 049470 ERS044049 ERR069743 CNSG02000001 CNSG02000138 23F   23F 629 629 629 9 741806 138 86199 1976 45 3
151684 151684 ERS044083 ERR069777 CNUI02000001 CNUI02000134 23F   23F 33 33 33 9 768253 134 113656 2003 41 3
203692 203692 ERS044096 ERR069790 CNTX02000001 CNTX02000130 23F   23F 36 36 36 9 723661 130 97257 2025 43 3
239434 239434 ERS044106 ERR069800 CFLL02000001 CFLL02000125 23F   23F 36 36 36 9 701687 125 95258 2032 42 3
365502 365502 ERS044136 ERR069830 CHBM02000001 CHBM02000160 23F   23F 439 439 439 9 625033 160 104195 2108 43 3
427937 427937 ERS044152 ERR065955 CNPX02000001 CNPX02000151 23F   23F 627 627 627 9 1410541 151 62540 2037 46 3
462956 462956 ERS044163 ERR065966 CNPO02000001 CNPO02000210 23F   23F 36 36 36 9 1471009 210 74439 2019 41 3
R34-3013 R34-3013 ERS043834 ERR065339 CNOW02000001 CNOW02000132 23F   23F 1908 1908 1908 9 484809 132 118278 2045 46 3
R34-3029 R34-3029 ERS043850 ERR065355 CNOZ02000001 CNOZ02000109 23F   23F 36 36 36 9 741213 109 118419 2016 45 3
R34-3076 R34-3076 ERS043895 ERR065331 CNOQ02000001 CNOQ02000102 23F   23F 36 36 36 9 546962 102 122873 1977 46 3
R34-3181 R34-3181 ERS043986 ERR067980 CNQP02000001 CNQP02000144 23F   23F 1909 1909 1909 9 583435 144 122802 1976 43 3
93209 093209 ERS044063 ERR069757 CNTL02000001 CNTL02000120 6C   6C 1379 1379 1379 10 764238 120 74728 1992 45 3
119571 119571 ERS044072 ERR069766 CNTA02000001 CNTA02000137 6C   6C 1390 1390 1390 10 778044 137 79162 1994 45 3
291880 291880 ERS044117 ERR069811 CNUO02000001 CNUO02000165 6C   6C 1390 1390 1390 10 629035 165 79160 2002 45 3
BR1045 NB8E4 ERS069930 ERR129039 COAN02000001 COAN02000156 6C   6C 1390 1390 1390 10 523591 156 65272 1999 43 3
BR1076 QQK9Y ERS069939 ERR129048 CRYL02000001 CRYL02000106 6C   6C 1379 1379 1379 10 551256 106 123360 1987 45 3
GL3032 SEOTT ERS070002 ERR129111 COCV02000001 COCV02000132 6C   6C 1379 1379 1379 10 633772 132 71318 1979 41 3
LE4079 ODNWT ERS070036 ERR129145 CFLY02000001 CFLY02000125 6C   6C 1379 1379 1379 10 630498 125 78375 2001 47 3
LE4100 V5VF1 ERS070043 ERR129152 CODV02000001 CODV02000116 6C   6C 1390 1390 1390 10 435645 116 88368 1999 45 3
MD5031 PHY03 ERS070060 ERR129169 COEL02000001 COEL02000167 6C   6C 1390 1390 1390 10 457980 167 71747 1992 34 3
ND6046 8CRCE ERS070094 ERR129203 COFK02000001 COFK02000146 6C   6C 1390 1390 1390 10 572506 146 65224 1994 45 3
ND6110 PWGX0 ERS070108 ERR124217 CNXT02000001 CNXT02000165 6C   6C 1379 1379 1379 10 1478923 165 62946 1984 40 3
ND6135 6PU39 ERS070123 ERR124232 CNXZ02000001 CNXZ02000170 6C   6C 1390 1390 1390 10 925659 170 51208 1989 38 3
NP7028 NKLJL ERS070144 ERR124253 CNYP02000001 CNYP02000211 6C   6C 1379 1379 1379 10 1202456 211 68216 2040 42 3
NP7029 3JK7Y ERS070145 ERR124254 CNYT02000001 CNYT02000174 6C   6C 1390 1390 1390 10 759341 174 54537 2041 37 3
NP7070 057G3 ERS070155 ERR124264 CNYU02000001 CNYU02000162 6C   6C 1390 1390 1390 10 1435872 162 48360 1997 41 3
NP7078 4XILA ERS070157 ERR124266 CNZA02000001 CNZA02000167 6C   6C 1379 1379 1379 10 734159 167 71318 1982 38 3
PT8081 LY288 ERS070196 ERR124305 CIIT02000001 CIIT02000152 6C   6C 1379 1379 1379 10 1309262 152 74740 1981 44 2
PT8092 QU2WM ERS070201 ERR124310 CHXC02000001 CHXC02000156 6C   6C 1379 1379 1379 10 1252811 156 69492 1990 38 3
PT8114 QD0ZV ERS070208 ERR124317 CHXJ02000001 CHXJ02000141 6C   6C 1390 1390 1390 10 501117 141 71207 1998 45 3
R34-3041 R34-3041 ERS043862 ERR065298 CNNM02000001 CNNM02000151 6C   6C 1390 1390 1390 10 1020710 151 71195 2007 45 3
R34-3060 R34-3060 ERS043880 ERR065316 CNOF02000001 CNOF02000144 6C   6C 1379 1379 1379 10 464158 144 74728 2046 45 3
R34-3072 R34-3072 ERS043891 ERR065327 CNOO02000001 CNOO02000138 6C   6C 1390 1390 1390 10 536053 138 68749 1993 45 3
R34-3089 R34-3089 ERS043908 ERR067994 CNVY02000001 CNVY02000202 6C   6C 1390 1390 1390 10 662413 202 66654 1986 41 3
R34-3092 R34-3092 ERS043911 ERR067997 CNWB02000001 CNWB02000152 Pool I 6C 6C 1390 1390 1390 10 695410 152 68969 2000 45 3
R34-3125 R34-3125 ERS043939 ERR068025 CNWP02000001 CNWP02000180 6C   6C 1390 1390 1390 10 641249 180 47929 2000 41 3
R34-3053 R34-3053 ERS043873 ERR065309 CNNZ02000001 CNNZ02000133 NT   6C 1379 1379 1379 10 581258 133 79177 2051 45 3
7649 007649 ERS044039 ERR069733 CNSZ02000001 CNSZ02000102 35B   35B 558 558 558 11 645576 102 80308 1928 45 3
67094 067094 ERS044055 ERR069749 CNSL02000001 CNSL02000116 35B   35B 1946 558 558 11 674433 116 68288 1903 44 3
379678 379678 ERS044140 ERR069834 CNUX02000001 CNUX02000158 35B   35B 558 558 558 11 618903 158 47273 1967 41 3
BR1029 T8Z8O ERS069926 ERR129035 COAL02000001 COAL02000109 35B   35B 558 558 558 11 480655 109 68301 1914 44 3
CH2016 F77ZH ERS069965 ERR129074 CHXB02000001 CHXB02000118 35B   35B 558 558 558 11 516125 118 80523 1968 42 3
CH2074 4K4C9 ERS069977 ERR129086 COCI02000001 COCI02000152 35B   35B 558 558 558 11 575562 152 53336 1921 40 3
CH2075 QWSZT ERS069978 ERR129087 COCD02000001 COCD02000108 35B   35B 558 558 558 11 524683 108 71794 1917 42 3
CH2083 4PYM0 ERS069980 ERR129089 COCG02000001 COCG02000169 35B   35B 558 558 558 11 621661 169 39912 1917 41 3
CH2123 WAMFH ERS069990 ERR129099 COCQ02000001 COCQ02000117 35B   35B 558 558 558 11 552266 117 64020 1918 44 3
GL3019 W8IHX ERS069995 ERR129104 COCS02000001 COCS02000166 35B   35B 558 558 558 11 600023 166 46254 1915 38 3
GL3061 9D25H ERS070010 ERR129119 CODF02000001 CODF02000144 35B   35B 558 558 558 11 642988 144 52254 1914 40 3
LE4021 P1NM2 ERS070025 ERR129134 CODJ02000001 CODJ02000133 35B   35B 3277 3277 3277 11 535581 133 53334 1925 40 3
LE4062 0U64I ERS070034 ERR129143 CODM02000001 CODM02000124 35B   35B 558 558 558 11 560663 124 67445 1916 40 3
LE4084 UTEDZ ERS070039 ERR129148 CODQ02000001 CODQ02000190 35B   35B 558 558 558 11 482101 190 43583 1912 30 3
MD5008 B1KMB ERS070054 ERR129163 COEK02000001 COEK02000161 35B   35B 558 558 558 11 557353 161 51757 1936 40 3
MD5042 AFVC5 ERS070064 ERR129173 COEP02000001 COEP02000138 35B   35B 558 558 558 11 513828 138 56186 1904 38 3
MD5081 REAOU ERS070070 ERR129179 CHXN02000001 CHXN02000128 35B   35B 558 558 558 11 525989 128 67445 1912 40 3
ND6038 0NWX9 ERS070091 ERR129200 COFI02000001 COFI02000147 35B   35B 558 558 558 11 536535 147 49320 1921 41 3
ND6141 1VDX8 ERS070127 ERR124236 CNYG02000001 CNYG02000171 35B   35B 558 558 558 11 890199 171 46882 1912 40 2
ND6153 CCV1H ERS070132 ERR124241 CNYD02000001 CNYD02000180 35B   35B 558 558 558 11 1356930 180 40436 1910 40 3
NP7020 W9GKO ERS070141 ERR124250 CNYR02000001 CNYR02000141 35B   35B 558 558 558 11 986715 141 67171 1988 41 0
NP7109 LS3OB ERS070164 ERR124273 CFJU02000001 CFJU02000211 35B   35B 558 558 558 11 1482671 211 39912 1912 40 2
NP7113 RS9D2 ERS070166 ERR124275 CNZF02000001 CNZF02000316 35B   35B 558 558 558 11 1675064 316 21028 1901 31 3
NP7122 6893Z ERS070168 ERR124277 CFQY02000001 CFQY02000193 35B   35B 558 558 558 11 845238 193 39914 1911 30 3
PT8003 81LMX ERS070170 ERR124279 CNZI02000001 CNZI02000208 35B   35B 558 558 558 11 786840 208 40209 1910 39 3
PT8035 BZ2I7 ERS070181 ERR124290 CNZO02000001 CNZO02000220 35B   35B 558 558 558 11 1119263 220 34345 1905 34 3
PT8040 O0RHB ERS070184 ERR124293 CHBS02000001 CHBS02000240 35B   35B 558 558 558 11 857518 240 32540 1920 30 3
PT8064 O61U7 ERS070190 ERR124299 CNZT02000001 CNZT02000253 35B   35B 558 558 558 11 1020004 253 31937 1910 29 3
PT8069 O8I1E ERS070192 ERR124301 CNZV02000001 CNZV02000240 35B   35B 558 558 558 11 1139763 240 30607 1906 32 3
PT8107 JBYFY ERS070207 ERR124316 COAG02000001 COAG02000101 35B   35B 558 558 558 11 390709 101 67446 1917 44 3
R34-3025 R34-3025 ERS043846 ERR065351 CNPL02000001 CNPL02000147 35B   35B 558 558 558 11 496882 147 49192 1914 43 3
R34-3044 R34-3044 ERS043864 ERR065300 CNNL02000001 CNNL02000120 35B   35B 558 558 558 11 552380 120 68288 1922 45 3
R34-3069 R34-3069 ERS043888 ERR065324 CNOL02000001 CNOL02000127 35B   35B 558 558 558 11 566136 127 68090 1930 41 3
R34-3074 R34-3074 ERS043893 ERR065329 CNOV02000001 CNOV02000167 NT 35B 35B 558 558 558 11 473228 167 43706 1920 38 3
R34-3083 R34-3083 ERS043902 ERR067988 CNWN02000001 CNWN02000132 35B   35B 558 558 558 11 671702 132 67433 1924 41 3
R34-3087 R34-3087 ERS043906 ERR067992 CNVX02000001 CNVX02000118 35B   35B 558 558 558 11 582794 118 68289 1920 41 3
R34-3097 R34-3097 ERS043916 ERR068002 CNWG02000001 CNWG02000226 35B   35B 558 558 558 11 659561 226 38025 1912 30 3
R34-3119 R34-3119 ERS043933 ERR068019 CNWT02000001 CNWT02000111 35B   35B 558 558 558 11 598720 111 80487 1962 45 3
R34-3131 R34-3131 ERS043943 ERR068029 CNXA02000001 CNXA02000127 35B   35B 558 558 558 11 683965 127 70434 1962 45 3
R34-3139 R34-3139 ERS043947 ERR068033 CNXR02000001 CNXR02000163 35B   35B 558 558 558 11 622738 163 45107 1914 41 3
R34-3164 R34-3164 ERS043968 ERR067962 CNQO02000001 CNQO02000143 35B Pool G 35B 1947 1947 1947 11 606548 143 51654 1922 40 3
R34-3165 R34-3165 ERS043969 ERR067963 CNQN02000001 CNQN02000116 35B   35B 558 558 558 11 581140 116 68288 1976 45 3
R34-3172 R34-3172 ERS043976 ERR067970 CNQC02000001 CNQC02000182 35B   35B 1947 3632 3632 11 547613 182 41812 1925 41 3
R34-3204 R34-3204 ERS044009 ERR069703 CNQS02000001 CNQS02000159 35B   35B 558 558 558 11 738391 159 67700 2012 41 3
R34-3227 R34-3227 ERS044032 ERR069726 CNRW02000001 CNRW02000170 35B   35B 558 558 558 11 777195 170 47314 1931 42 3
R34-3229 R34-3229 ERS044034 ERR069728 CNRY02000001 CNRY02000138 35B   35B 558 558 558 11 807583 138 56108 1914 42 3
255210 255210 ERS044109 ERR069803 CNVE02000001 CNVE02000206   NT NT   449 449 12 521097 206 39906 2097 45 3
403790 403790 ERS044147 ERR069841 CNVO02000001 CNVO02000197 NT   NT   1054 7803 12 621882 197 40790 2040 45 3
462746 462746 ERS044162 ERR065965 CNPK02000001 CNPK02000180 NT   NT 448 448 448 12 1434835 180 54927 2084 45 3
508907 508907 ERS044171 ERR065974 CNPU02000001 CNPU02000205 NT   NT 448 448 448 12 1479387 205 54929 2081 45 3
LE4012 68G2D ERS070021 ERR129130 CODC02000001 CODC02000201 NT   NT 62 448 448 12 765506 201 53870 2087 34 3
PT8000 WVCE6 ERS070169 ERR124278 CFQQ02000001 CFQQ02000209 NT   NT 3278 448 448 12 1107892 209 49980 2081 44 3
R34-3015 R34-3015 ERS043836 ERR065341 CNPF02000001 CNPF02000171 NT   NT 448 448 448 12 554434 171 50610 2077 45 3
R34-3032 R34-3032 ERS043853 ERR065289 CFQL02000001 CFQL02000166 Pool I NT NT   448 448 12 664900 166 53916 2089 45 3
R34-3039 R34-3039 ERS043860 ERR065296 CNNO02000001 CNNO02000165 6B NT NT 448 448 448 12 646546 165 54902 2086 45 3
R34-3088 R34-3088 ERS043907 ERR067993 CFJY02000001 CFJY02000211   NT NT   344 344 12 814267 211 40190 2089 45 3
15445 015445 ERS044040 ERR069734 CNTB02000001 CNTB02000210 6A   6B 1876 1876 1876 13 710738 210 50116 1983 42 3
42861 042861 ERS044047 ERR069741 CNSE02000001 CNSE02000269 6A   6B 1911 10036 10036 13 685819 269 39880 1983 32 3
101058 101058 ERS044065 ERR069759 CHBL02000001 CHBL02000162 6A   6B 690 690 690 13 882416 162 59787 1989 52 3
166637 166637 ERS044090 ERR069784 CNTU02000001 CNTU02000140   6 6B   473 473 13 658118 140 63964 2000 46 3
181272 181272 ERS044094 ERR069788 CNTW02000001 CNTW02000159 6A   6B 1876 1876 1876 13 701590 159 59255 1994 46 3
359370 359370 ERS044133 ERR069827 CNVT02000001 CNVT02000171 6A   6B 1926 1876 1876 13 740093 171 57397 1991 46 3
420881 420881 ERS044150 ERR065953 CIDS02000001 CIDS02000271 6A   6B 1940 1940 1940 13 1327340 271 39783 1986 31 3
CH2090 0TN5H ERS069983 ERR129092 COCJ02000001 COCJ02000186 6A   6B 1876 1876 1876 13 566419 186 53289 2035 44 3
R34-3027 R34-3027 ERS043848 ERR065353 CNPD02000001 CNPD02000178 6A   6B 473 473 473 13 563530 178 54094 1987 42 3
R34-3096 R34-3096 ERS043915 ERR068001 CNWF02000001 CNWF02000192 6A   6B 1928 1928 1928 13 643363 192 56978 1976 42 3
R34-3099 R34-3099 ERS043918 ERR068004 CFIP02000001 CFIP02000154 6A   6B 1927 1927 1927 13 714180 154 65660 2058 46 3
R34-3151 R34-3151 ERS043957 ERR068043 CNXG02000001 CNXG02000253 6A   6B 1917 1876 1876 13 622561 253 46473 1982 44 3
R34-3180 R34-3180 ERS043985 ERR067979 CNQL02000001 CNQL02000260 6A   6B 690 690 690 13 554946 260 40320 1977 40 3
R34-3210 R34-3210 ERS044015 ERR069709 CHIO02000001 CHIO02000157 6A   6B 1876 1876 1876 13 752877 157 63730 2001 46 3
R34-3211 R34-3211 ERS044016 ERR069710 CNSA02000001 CNSA02000196 6A   6B 1876 1876 1876 13 664068 196 54094 1983 39 3
R34-3100 R34-3100 ERS043919 ERR068005 CHWV02000001 CHWV02000159 6B   6B 690 690 690 13 621229 159 59787 1996 52 3
BR1008 CM917 ERS069918 ERR129027 COBB02000001 COBB02000217 6C   6C 473 473 473 13 608916 217 48780 1994 40 2
GL3043 FU8OL ERS070006 ERR129115 COCZ02000001 COCZ02000216 6C   6C 473 473 473 13 488273 216 48037 2049 42 3
ND6119 5W908 ERS070114 ERR124223 CNYX02000001 CNYX02000180 6C   6C 473 473 473 13 898992 180 50284 1990 45 3
51715 051715 ERS044050 ERR069744 CNSO02000001 CNSO02000158 6A   6A 376 376 376 14 842144 158 54645 2041 42 3
65435 065435 ERS044053 ERR069747 CNSN02000001 CNSN02000162 6A   6A 376 376 376 14 715450 162 58082 2004 42 3
108480 108480 ERS044069 ERR069763 CNSR02000001 CNSR02000161 6A   6A 376 376 376 14 907154 161 57928 2014 42 3
179743 179743 ERS044093 ERR069787 CNTV02000001 CNTV02000163 6A   6A 376 376 376 14 714250 163 55893 2006 42 3
342672 342672 ERS044131 ERR069825 CNVS02000001 CNVS02000175 6A   6A 1922 1922 1922 14 584422 175 46950 1992 42 3
448643 448643 ERS044160 ERR065963 CNPN02000001 CNPN02000186 6A   6A 376 376 376 14 1238469 186 40684 2038 38 3
LE4007 CXQ2T ERS070019 ERR129128 COEM02000001 COEM02000189 6A   6B 1538 1538 1538 14 538853 189 40812 2047 38 3
PT8074 AI3GZ ERS070194 ERR124303 CHBU02000001 CHBU02000177 6A   6A 376 376 376 14 865534 177 54657 2033 43 3
PT8105 2RLC4 ERS070205 ERR124314 COAE02000001 COAE02000167 6A   6B 376 376 376 14 545326 167 54654 2044 35 3
R34-3168 R34-3168 ERS043972 ERR067966 CNQT02000001 CNQT02000231 6A   6A 376 376 376 14 544245 231 40800 2029 37 3
R34-3171 R34-3171 ERS043975 ERR067969 CNQE02000001 CNQE02000256 6A   6A 376 376 376 14 521771 256 34437 2024 34 3
R34-3201 R34-3201 ERS044006 ERR069700 CNQW02000001 CNQW02000360 6A   6A 1921 1921 1921 14 808590 360 22244 2045 30 3
BR1022-1 DH8X5-1 ERS069922 ERR129031 COBR02000001 COBR02000251 19A   19A 320 320 320 15 570719 251 33714 1976 36 3
BR1036 06URQ ERS069927 ERR129036 COAJ02000001 COAJ02000232 19A   19A 320 320 320 15 519594 232 39129 1977 43 3
BR1080 NFPTS ERS069940 ERR129049 CFQT02000001 CFQT02000156 19A   19A 320 320 320 15 544707 156 59177 1974 47 3
CH2017 1QJAP ERS069966 ERR129075 COBV02000001 COBV02000248 19A   19A 320 320 320 15 519029 248 35971 1938 34 3
ND6010 9SKOT ERS070084 ERR129193 COFD02000001 COFD02000160 19A   19A 320 320 320 15 515723 160 55884 1977 42 3
ND6118 OQTRJ ERS070113 ERR124222 CNYW02000001 CNYW02000166 19A   19A 320 320 320 15 883212 166 48716 1938 43 3
ND6137 LABTO ERS070124 ERR124233 CNXV02000001 CNXV02000149 19A   19A 320 320 320 15 865637 149 56124 1969 45 3
NP7047 CHP0M ERS070146 ERR124255 CNYL02000001 CNYL02000337 19A   19A 1451 1451 1451 15 515569 337 21839 1998 31 3
R34-3221 R34-3221 ERS044026 ERR069720 CNRR02000001 CNRR02000170 19A   19A 320 320 320 15 732768 170 59783 1939 42 3
105542 105542 ERS044068 ERR069762 CFQW02000001 CFQW02000161 9A 19F 19F 1945 1945 1945 15 704891 161 57389 1952 46 2
208866 208866 ERS044097 ERR069791 CNUA02000001 CNUA02000163 19F   19F 236 236 236 15 711884 163 62950 1946 46 3
209930 209930 ERS044099 ERR069793 CNUB02000001 CNUB02000179 19F   19F 236 236 236 15 692143 179 48868 1942 44 3
404463 404463 ERS044148 ERR069842 CHBN02000001 CHBN02000173 19F   19F 236 236 236 15 807881 173 56367 1942 46 3
416185 416185 ERS044149 ERR069843 CNVP02000001 CNVP02000227 19F   19F 320 320 320 15 747175 227 56601 1983 41 3
503574 503574 ERS044170 ERR065973 CNPT02000001 CNPT02000162 19F   19F 320 320 320 15 1443326 162 58805 1986 44 3
BR1111 6GU7V ERS069953 ERR129062 COBF02000001 COBF02000183 19F   19F 3292 3292 3292 15 553921 183 55163 2000 43 3
CH2033 WMK3T ERS069969 ERR129078 CFRJ02000001 CFRJ02000186 19F   19F 236 236 236 15 531232 186 50718 1973 42 3
R34-3058 R34-3058 ERS043878 ERR065314 CNOD02000001 CNOD02000172 19F   19F 236 236 236 15 444412 172 57390 1992 46 3
R34-3085 R34-3085 ERS043904 ERR067990 CHBQ02000001 CHBQ02000269 19F   19F 271 271 271 15 790622 269 33792 1972 38 3
R34-3094 R34-3094 ERS043913 ERR067999 CHBR02000001 CHBR02000162 19F   19F 236 236 236 15 671606 162 57389 2016 47 3
R34-3098 R34-3098 ERS043917 ERR068003 CNWH02000001 CNWH02000166 19F   19F 1941 1941 1941 15 597150 166 62940 1949 47 3
R34-3141 R34-3141 ERS043949 ERR068035 CNXS02000001 CNXS02000299 19F   19F 1937 271 271 15 662233 299 28410 1974 31 3
R34-3142 R34-3142 ERS043950 ERR068036 CFRK02000001 CFRK02000305 19F   19F 271 271 271 15 684428 305 24262 1969 31 3
R34-3200 R34-3200 ERS044005 ERR069699 CHBG02000001 CHBG02000149 19F   19F 236 236 236 15 779663 149 57389 1959 46 3
R34-3207 R34-3207 ERS044012 ERR069706 CHWU02000001 CHWU02000166 19F   19F 1943 1943 1943 15 804061 166 56376 1989 45 3
65645 065645 ERS044054 ERR069748 CNSP02000001 CNSP02000120 3   3 180 180 180 16 752562 120 90828 2001 46 3
72511 072511 ERS044056 ERR069750 CNSV02000001 CNSV02000124   3 3   180 180 16 897874 124 91041 1953 43 3
369820 369820 ERS044138 ERR069832 CNVG02000001 CNVG02000119 3   3 180 180 180 16 826096 119 95228 1987 39 3
MD5090 P213M ERS070071 ERR129180 COEV02000001 COEV02000122 3   3 180 180 180 16 483715 122 97568 2003 43 3
ND6097 LDD87 ERS070103 ERR129212 COFU02000001 COFU02000128 3   3 180 180 180 16 1020942 128 91059 1987 42 1
R34-3078 R34-3078 ERS043897 ERR067983 CNVW02000001 CNVW02000124 3   3 180 180 180 16 712646 124 91025 1950 43 3
R34-3084 R34-3084 ERS043903 ERR067989 CFLM02000001 CFLM02000251 3   3 180 180 180 16 762742 251 58581 2046 41 3
R34-3101 R34-3101 ERS043920 ERR068006 CNWU02000001 CNWU02000122 3   3 180 180 180 16 679544 122 91029 1950 43 3
R34-3190 R34-3190 ERS043995 ERR069689 CNRJ02000001 CNRJ02000110 3   3 180 180 180 16 738923 110 96268 1984 44 3
R34-3202 R34-3202 ERS044007 ERR069701 CNRH02000001 CNRH02000134 3   3 180 180 180 16 805360 134 91041 1995 43 3
R34-3217 R34-3217 ERS044022 ERR069716 CNRP02000001 CNRP02000117 3   3 180 180 180 16 650169 117 91029 1956 43 3
142534 142534 ERS044078 ERR069772 CNTO02000001 CNTO02000185 14   14 124 124 124 16 785710 185 38979 1994 46 3
268660 268660 ERS044112 ERR069806 CNVB02000001 CNVB02000174 14   14 124 124 124 16 662547 174 40732 1989 45 3
306435 306435 ERS044122 ERR069816 CNUV02000001 CNUV02000182 14   14 124 124 124 16 578420 182 40732 1997 45 3
341365 341365 ERS044130 ERR069824 CNVR02000001 CNVR02000197 14   14 124 124 124 16 713927 197 43426 2054 46 3
430772 430772 ERS044153 ERR065956 CIIR02000001 CIIR02000188 14   14 124 124 124 16 1217194 188 40138 1998 46 3
R34-3230 R34-3230 ERS044035 ERR069729 CNSD02000001 CNSD02000182 14   14 124 124 124 16 840724 182 40732 2030 45 3
R34-3231 R34-3231 ERS044036 ERR069730 CNSU02000001 CNSU02000184 14   14 124 124 124 16 715524 184 55853 2038 46 3
GL3031 VODW3 ERS070001 ERR129110 COCU02000001 COCU02000138 21   21 432 432 432 16 569706 138 67367 2042 44 3
BR1101 E0ZKK ERS069948 ERR129057 COBA02000001 COBA02000131 31   31 568 568 568 16 545690 131 52285 1957 44 3
LE4030 N8YA4 ERS070026 ERR129135 CODH02000001 CODH02000119 31   31 568 568 568 16 524480 119 52473 1952 44 3
PT8093 YSOFP ERS070202 ERR124311 COAC02000001 COAC02000120 31   31 568 568 568 16 1058147 120 52277 1960 45 3
R34-3033 R34-3033 ERS043854 ERR065290 CNNK02000001 CNNK02000130 31   31 568 568 568 16 616222 130 52596 1957 45 3
438180 438180 ERS044156 ERR065959 CNQA02000001 CNQA02000194 34   34 547 547 547 16 1396389 194 59325 2070 41 3
BR1041 NA03L ERS069929 ERR129038 COAP02000001 COAP02000183 34   34 547 547 547 16 542908 183 73649 2045 40 3
BR1115 DR1PD ERS069957 ERR129066 COBK02000001 COBK02000126 34   34 547 547 547 16 563613 126 76434 2046 45 3
R34-3048 R34-3048 ERS043868 ERR065304 CNNV02000001 CNNV02000142 34   34 1931 547 547 16 701664 142 73930 2067 45 3
R34-3115 R34-3115 ERS043930 ERR068016 CNWK02000001 CNWK02000142 34   34 547 547 547 16 609572 142 76543 2067 45 3
R34-3176 R34-3176 ERS043981 ERR067975 CNQH02000001 CNQH02000237 34   34 1902 1902 1902 16 663022 237 44714 2096 39 3
304793 304793 ERS044121 ERR069815 CNUY02000001 CNUY02000166 9N Pool D 37 447 447 6942 16 596507 166 48962 1946 43 3
LE4099 F5UY4 ERS070042 ERR129151 CODT02000001 CODT02000143 37   37 447 447 6942 16 417920 143 49010 1942 45 3
147587 147587 ERS044081 ERR069775 CNTR02000001 CNTR02000264 38   38 393 393 393 16 741838 264 45303 2036 38 3
BR1048 HU4AA ERS069931 ERR129040 COAO02000001 COAO02000265 38   38 393 393 393 16 534430 265 32187 2068 38 3
BR1098 8PNF1 ERS069947 ERR129056 CFRF02000001 CFRF02000204 38   38 393 393 393 16 552230 204 39157 2023 42 2
GL3078 J29X8 ERS070015 ERR129124 COEC02000001 COEC02000189 NT 38 38 393 393 393 16 533034 189 45340 2047 45 3
ND6005 K8121 ERS070079 ERR129188 COFC02000001 COFC02000197 38   38 393 393 393 16 483648 197 39628 2054 45 3
R34-3046 R34-3046 ERS043866 ERR065302 CNNN02000001 CNNN02000235 38   38 393 393 393 16 587582 235 45392 2047 41 3
R34-3065 R34-3065 ERS043885 ERR065321 CNOJ02000001 CNOJ02000199 25A 38 38 393 393 393 16 531234 199 49022 2051 45 3
R34-3146 R34-3146 ERS043953 ERR068039 CNXC02000001 CNXC02000195 6C 38 38 1390 393 393 16 571351 195 45320 2038 45 3
277394 277394 ERS044114 ERR069808 CNUQ02000001 CNUQ02000206 11A   11D 1934 7661 7661 16 610477 206 57136 2088 42 3
333793 333793 ERS044126 ERR069820 CNUP02000001 CNUP02000141 15A   15A 817 817 817 16 733666 141 56397 1954 45 3
NP7004 18FDW ERS070135 ERR124244 CNYA02000001 CNYA02000142 15B/C   15C 346 346 346 16 895142 142 63816 2005 43 3
BR1050 X703J ERS069932 ERR129041 COAQ02000001 COAQ02000169 16F   16F 995 995 995 16 597707 169 65882 2021 43 3
MD5096 2W7ME ERS070072 ERR129181 COET02000001 COET02000149 16F   16F 570 570 570 16 527284 149 80141 2023 34 3
MD5106 SHTPB ERS070075 ERR129184 COEX02000001 COEX02000167 16F   16F 659 659 659 16 538101 167 74046 2072 44 3
NP7012 M66VO ERS070138 ERR124247 CNYJ02000001 CNYJ02000197 16F   16F 1840 1840 1840 16 1444341 197 63248 2086 44 3
NP7103 2Y1CQ ERS070162 ERR124271 CNZD02000001 CNZD02000133 16F   16F 659 659 659 16 711094 133 73934 1961 44 3
PT8029 RD7KX ERS070180 ERR124289 CNZM02000001 CNZM02000203 16F   16F 659 659 659 16 914843 203 52928 2061 39 3
PT8090 BA5TC ERS070200 ERR124309 CNZY02000001 CNZY02000191 16F   16F 659 659 659 16 1161912 191 57166 2078 45 3
R34-3062 R34-3062 ERS043882 ERR065318 CNOH02000001 CNOH02000180 16F   16F 659 659 659 16 471228 180 80496 1976 45 3
ND6111 GWTK7 ERS070109 ERR124218 CHBP02000001 CHBP02000162 17F   17F 392 392 392 16 1436662 162 57903 1963 44 3
NP7026 9LUM5 ERS070143 ERR124252 CFRB02000001 CFRB02000133 17F   17F 2355 2355 8563 16 1437060 133 74364 1954 46 3
NP7057 3XUC4 ERS070149 ERR124258 CNYS02000001 CNYS02000142 17F   17F 392 392 392 16 841522 142 66633 1971 43 3
PT8082 E3GXY ERS070197 ERR124306 CIEC02000001 CIEC02000145 17F   17F 392 392 392 16 1344486 145 48946 1986 45 3
R34-3056 R34-3056 ERS043876 ERR065312 CNOE02000001 CNOE02000138 17F   17F 1924 1924 1924 16 442380 138 65850 1996 45 3
40241 040241 ERS044045 ERR069739 CNSF02000001 CNSF02000205 18C   18C 1923 1923 1923 16 797963 205 59905 2070 46 3
303656 303656 ERS044120 ERR069814 CHBK02000001 CHBK02000135 18C   18C 1948 496 496 16 652704 135 75300 1997 45 3
R34-3059 R34-3059 ERS043879 ERR065315 CNOG02000001 CNOG02000176 37 18C 18C 1073 1073 1073 16 494629 176 79858 2069 46 3
R34-3102 R34-3102 ERS043921 ERR068007 CNWR02000001 CNWR02000142 18C   18C 496 496 496 16 589985 142 80570 1992 44 3
R34-3196 R34-3196 ERS044001 ERR069695 CHWS02000001 CHWS02000185 18C   18C 113 113 113 16 770603 185 88139 2024 47 3
BR1012 26IET ERS069919 ERR129028 COBH02000001 COBH02000183 19A   19A 695 SLV695 695 16 549104 183 39572 2072 47 3
BR1019 FRIZ4 ERS069921 ERR129030 COBN02000001 COBN02000184 19A   19A 36 SLV695 695 16 516806 184 39572 2071 46 3
CH2067 X71H5 ERS069974 ERR129083 COBZ02000001 COBZ02000203 19A   19A 695 SLV695 695 16 566926 203 42951 2080 46 3
CH2069 7NA98 ERS069975 ERR129084 COCB02000001 COCB02000143 19A   19A 415 415 415 16 698246 143 56417 1994 44 3
GL3015 Y6BSG ERS069994 ERR129103 COCR02000001 COCR02000228 19A   19A 695 SLV695 695 16 583440 228 39580 2132 46 3
LE4038 3UTO8 ERS070027 ERR129136 CODN02000001 CODN02000176 19A   19A 695 SLV695 695 16 539693 176 37541 2072 46 3
LE4047 6U8ZJ ERS070029 ERR129138 CODL02000001 CODL02000201 19A   19A 180 SLV695 695 16 1027130 201 35497 2067 46 2
ND6042 3GWV1 ERS070093 ERR129202 COFP02000001 COFP02000222 19A   19A 695 SLV695 695 16 501324 222 42927 2118 46 3
ND6109 MFII0 ERS070107 ERR129216 COFY02000001 COFY02000174 19A   19A 695 SLV695 695 16 351551 174 42951 2080 46 3
R34-3037 R34-3037 ERS043858 ERR065294 CNNS02000001 CNNS02000203 19A   19A 1944 1944 1944 16 557513 203 48681 2038 41 3
R34-3183 R34-3183 ERS043988 ERR067982 CFQP02000001 CFQP02000130 19A   19A 276 276 10194 16 602182 130 78511 1994 45 3
27999 027999 ERS044043 ERR069737 CNSS02000001 CNSS02000273 19F   19F 1340 1340 1340 16 782294 273 57970 2124 46 3
209464 209464 ERS044098 ERR069792 CNTY02000001 CNTY02000196 19F   19F 425 425 425 16 696536 196 46435 1993 45 3
284968 284968 ERS044116 ERR069810 CNUL02000001 CNUL02000174 19F   19F 425 425 425 16 649536 174 47632 1996 46 3
439699 439699 ERS044157 ERR065960 CFQS02000001 CFQS02000201 19F   19F 654 654 654 16 1403097 201 48219 2008 46 3
BR1065 DALR8 ERS069936 ERR129045 COAT02000001 COAT02000262 19F   19F 426 426 426 16 547973 262 42584 2126 42 3
BR1081 SQADW ERS069941 ERR129050 COAW02000001 COAW02000178 19F   19F 654 654 654 16 551841 178 46467 2012 47 3
CH2105 QIKYS ERS069985 ERR129094 COCL02000001 COCL02000190 19F 19F 19F 43 43 43 16 560697 190 44749 2031 45 3
CH2106 Q0413 ERS069986 ERR129095 CXOE01000001 CXOE01000178 NT 19F 19F 43 43 43 16 530890 178 48337 2021 46 3
ND6052 TJRUQ ERS070097 ERR129206 COFM02000001 COFM02000200 19F   19F 43 43 43 16 629196 200 69065 2035 37 3
R34-3028 R34-3028 ERS043849 ERR065354 CNOX02000001 CNOX02000178 19F   19F 425 425 425 16 606774 178 47404 2050 45 3
R34-3043 R34-3043 ERS043863 ERR065299 CNNR02000001 CNNR02000179 19F   19F 654 654 654 16 556874 179 48619 1964 46 3
R34-3070 R34-3070 ERS043889 ERR065325 CHIN02000001 CHIN02000226 19F   19F 654 654 654 16 506861 226 46277 2053 47 3
R34-3113 R34-3113 ERS043928 ERR068014 CNWZ02000001 CNWZ02000181 19F   19F 43 43 43 16 579564 181 58752 1970 45 3
R34-3137 R34-3137 ERS043945 ERR068031 CFIQ02000001 CFIQ02000190 19F   19F 425 425 425 16 678105 190 51413 2053 46 3
R34-3193 R34-3193 ERS043998 ERR069692 CNQY02000001 CNQY02000181 19F   19F 1903 1903 1903 16 721954 181 50254 2001 45 3
R34-3198 R34-3198 ERS044003 ERR069697 CNQZ02000001 CNQZ02000187 19F   19F 425 425 425 16 656612 187 50412 2061 47 3
R34-3224 R34-3224 ERS044029 ERR069723 CNRV02000001 CNRV02000167 19F   19F 425 425 425 16 783924 167 61628 2011 45 3
44744 044744 ERS044048 ERR069742 CNSJ02000001 CNSJ02000175   23 23F   242 242 16 784856 175 63365 2064 46 3
173015 173015 ERS044091 ERR069785 CNTS02000001 CNTS02000162 23F   23F 81 81 81 16 772262 162 69767 2053 45 3
218229 218229 ERS044103 ERR069797 CNUC02000001 CNUC02000187 23F   23F 81 81 81 16 760163 187 48689 2058 45 3
334847 334847 ERS044127 ERR069821 CNVF02000001 CNVF02000177 23F   23F 81 81 81 16 695521 177 58491 2059 45 3
144370 144370 ERS044079 ERR069773 CNTF02000001 CNTF02000231 25A   38 393 393 393 16 916919 231 36104 2044 35 3
MD5107 OZJ9F ERS070076 ERR129185 COEZ02000001 COEZ02000100 33A   33F 2705 2705 2705 16 328196 100 122092 2022 44 3
187406 187406 ERS044095 ERR069789 CIEE02000001 CIEE02000122 33   33F 100 100 100 16 591847 122 84375 1992 43 3
NP7053 ZPN7P ERS070148 ERR124257 CNYQ02000001 CNYQ02000120 33F   33F 2705 2705 2705 16 829937 120 97009 2013 44 3
R34-3121 R34-3121 ERS043935 ERR068021 CNWQ02000001 CNWQ02000125 33F   33F 100 100 100 16 692351 125 74621 1940 45 3
R34-3173 R34-3173 ERS043978 ERR067972 CNQD02000001 CNQD02000128 33F   33F 100 100 100 16 706317 128 75405 1982 45 3
R34-3023 R34-3023 ERS043844 ERR065349 CNPM02000001 CNPM02000184 35B   35B 452 452 452 16 534120 184 50437 1968 45 3
R34-3122 R34-3122 ERS043936 ERR068022 CVKI02000001 CVKI02000195 35B   35B 452 452 452 16 673207 195 54480 1965 45 3
R34-3223 R34-3223 ERS044028 ERR069722 CNRT02000001 CNRT02000180 35B   35B 452 452 452 16 784915 180 58183 1976 45 3
110093 110093 ERS044070 ERR069764 CNSQ02000001 CNSQ02000259 6A   6B 1939 1939 1939 16 805312 259 46223 2074 46 3
R34-3026 R34-3026 ERS043847 ERR065352 CNPC02000001 CNPC02000252 6A   6A 242 242 242 16 578246 252 40403 2030 41 3
18044 018044 ERS044041 ERR069735 CNTD02000001 CNTD02000174 6B   6C 1518 1518 1518 16 837914 174 52440 2077 46 3
160986 160986 ERS044088 ERR069782 CNUM02000001 CNUM02000168 6B   6A 1536 1536 1536 16 748659 168 68783 2007 45 3
302649 302649 ERS044119 ERR069813 CNUW02000001 CNUW02000187 6B   6A 146 146 146 16 671199 187 57742 2059 45 3
323485 323485 ERS044125 ERR069819 CFQU02000001 CFQU02000215 6B   NT 146 146 146 16 627195 215 50634 2047 41 3
388483 388483 ERS044144 ERR069838 CNVK02000001 CNVK02000216 6B   NT 146 146 146 16 789543 216 40936 1998 34 3
397079 397079 ERS044146 ERR069840 CNVN02000001 CNVN02000192 6B   6A 315 315 315 16 701215 192 40720 2132 45 3
436154 436154 ERS044154 ERR065957 CNPY02000001 CNPY02000261 6B   6A 1954 1954 1954 16 1237316 261 46510 2083 41 3
R34-3038 R34-3038 ERS043859 ERR065295 CHBE02000001 CHBE02000186 6B   6A 1930 1930 1930 16 712729 186 55199 2057 46 3
BR1064 EKR1O ERS069935 ERR129044 COAS02000001 COAS02000225 6C   6C 1692 1692 1692 16 534817 225 34151 2003 31 3
CH2006 WG60C ERS069962 ERR129071 COBJ02000001 COBJ02000123 6C NT NT 547 3288 3288 16 623754 123 54052 2001 43 2
ND6012 P0N8K ERS070085 ERR129194 COFE02000001 COFE02000225 6C   6C 1692 1692 1692 16 471694 225 39894 1995 31 3
ND6133 6JT2I ERS070121 ERR124230 CNXY02000001 CNXY02000168 6C   6C 547 547 547 16 771433 168 66394 2109 46 3
R34-3086 R34-3086 ERS043905 ERR067991 CNWO02000001 CNWO02000172 6C   6C 547 547 547 16 718583 172 80401 2107 43 3
BR1074 2VBTK ERS069938 ERR129047 COAV02000001 COAV02000280 7C   7C 3284 3284 3284 16 582701 280 34224 1953 32 3
ND6000 W9G4K ERS070078 ERR129187 COFA02000001 COFA02000163 7C   7C 3283 1797 1797 16 575295 163 47752 2018 44 3
BR1113 U3EO1 ERS069955 ERR129064 COBG02000001 COBG02000094 7F   7A 1176 1176 1176 16 515607 94 65255 1925 46 3
132571 132571 ERS044075 ERR069769 CNTM02000001 CNTM02000126 9N   9N 405 405 405 16 726669 126 73240 1988 45 3
436915 436915 ERS044155 ERR065958 CNPZ02000001 CNPZ02000168 9N   9N 66 66 66 16 1343098 168 80750 1989 46 3
MD5007 GJ6MQ ERS070053 ERR129162 COEF02000001 COEF02000152 9N   9N 1797 66 66 16 512782 152 78813 1987 45 3
ND6123 14YE5 ERS070117 ERR124226 CFJW02000001 CFJW02000135 9N   9N 405 405 405 16 1035462 135 61282 1980 42 3
GL3012 34YLE ERS069993 ERR129102 COCP02000001 COCP02000142 NT   NT 3288 3288 3288 16 535078 142 57331 2011 47 3
R34-3108 R34-3108 ERS043923 ERR068009 CNWW02000001 CNWW02000174 NT   NT 1905 2011 2011 16 635137 174 58280 1990 43 3

Overnight plate growth was harvested through resuspension in phosphate buffered saline, and genomic DNA was extracted using DNeasy columns (Qiagen) following manufacturer’s instructions. The concentration of genomic DNA was quantified using the Qubit system (Life Technologies); all samples yielded at least 3 μg of DNA. The integrity of the genomic DNA was checked using agarose gel electrophoresis relative to a λHindIII ladder (New England Biolabs).

Generation of sequence data

Illumina sequencing libraries were constructed as described previously20,21. Briefly, genomic DNA was first fragmented using Adaptive Focused Acoustics technology (Covaris). The resulting fragments were then repaired to ensure they had blunt ends, phosphorylated at their 5′ end, A-tailed at the 3′ end, and ligated to adapter molecules. This library of fragments was then separated by agarose gel electrophoresis. DNA constructs of the appropriate size range (generating an insert size of approximately 150–300 bp) were then extracted from the gel and amplified by a polymerase chain reaction using Kapa HiFi polymerase (Kapa Biosystems) that added one of the 96 index tags used in this project. Libraries were then quantified using qPCR, and combined into an equimolar pool of 96 samples prior to denaturation, cluster generation and sequencing in a single flow cell lane with an Illumina HiSeq machine. Isolates from 2001 and 2003–2004 were sequenced as paired end libraries generating 75 nt reads; isolates from 2006–2007 were sequenced as paired end libraries generating 100 nt reads.

Assembly and annotation of sequence data

Sequences were assembled de novo using Velvet22 version 1.2 with parameters selected to be optimal for individual datasets as described previously23. Both Glimmer3 (ref. 24) and Prodigal25 were trained on the reference sequence of S. pneumoniae ATCC 700669 (ref. 26; Data Citation 1), then applied to the complete draft assembly with an 11 nt sequence encoding stop codons in each reading frame added to each end, to facilitate the identification of partial coding sequences (CDSs) broken by the assembly. Putative CDSs were then trimmed at the 3′ end to stop them spanning contig breaks within the assembly. Final CDS predictions were identified as the consensus of Glimmer3 and Prodigal outputs, as described previously19 (Table 1 (available online only)). Protein sequences were then translated, aligned using BLAT27 suite 0.34, and ‘clusters of orthologous genes’ (COGs) identified using COGsoft28. Pairs of orthologous sequences were then manually identified as described previously19.

To generate functional annotations of genome sequences, all CDSs were labelled with a unique identifier (of the form, ‘ERSX_Y’, where ‘ERSX’ is the sample accession code in the European Nucleotide Archive listed in Table 1 (available online only) and Y is an incrementing index) and their COG (of the form, ‘SPARC1_CLSZ’ or ‘SPARC1_CLSTZ’, where Z is a number). COGs relating to antibiotic resistance and the newly-characterised variable restriction-modification system loci were annotated as described previously29; the 590 COGs found to be specific to prophage, the three COGs found to be specific to a particular prophage remnant, the 142 COGs found to be specific to phage-related chromosomal islands, and the 355 COGs found to be specific to integrative and conjugative elements were also appropriately identified in these datasets29. All COGs not belonging to one of these categories were annotated using a database of pneumococcal CDS information. This was constructed by extracting the protein sequences and annotated functions from publicly-available complete genomes and the annotation of 90 capsule polysaccharide synthesis loci30. Where a CDS in one of the draft genomes had a putative protein sequence identical to the translated sequence of a previously annotated locus, the annotation was directly transferred; otherwise, the annotation was transferred on the basis of orthology, if another putative protein in the same COG was identical to the translation of a CDS in an annotated genome sequence. In cases where no such information could be obtained, CDSs were labelled as producing ‘hypothetical proteins’. Pneumococcal small interspersed repeats were annotated as described previously31, and tRNA and rRNA loci were annotated with tRNAscan-SE32 version 1.3.1 and rnammer33 version 1.2, respectively (Table 1 (available online only)).

Generation of core genome alignment and overall phylogeny

As described previously19, the 1,194 COGs found to have a single representative in each of the 616 genomes were individually aligned at the protein level using MUSCLE34, prior to backtranslation to generate a 1.14 Mb codon alignment. The 106,196 polymorphic sites were extracted and used to generate a phylogeny using RAxML35 version 7.0.4 with the general time reversible substitution model and a four category gamma distribution to account for rate heterogeneity. This tree was midpoint rooted on the longest branch, which separated sequence cluster 12 from the rest of the population. This is consistent with a wider phylogenetic analysis of multiple species that suggested sequence cluster 12 was the earliest lineage to diverge from the other isolates19. The same alignment was analysed with BAPS36 version 5 to identify the sequence clusters. Both the core genome alignment and tree are made available as part of Data Citation 2.

Generation of whole genome alignments

For each of the fifteen monophyletic sequence clusters identified using BAPS and RAxML, a single reference draft assembly was selected for manual curation. The Illumina read data were reassembled with SGA37 version 0.9, and these contigs merged with those from Velvet using Zorro38 version 2.2. These were arranged into scaffolds using SSPACE39 version 2, then manually curated and ordered using ABACAS40 and ACT41. These fifteen assemblies are made available as part of Data Citation 2. Illumina read pairs from isolates of the same sequence cluster were then mapped against this reference using SMALT42 version 0.5.8. The resulting read alignment was processed with Samtools43, VCFtools44 and Biopython45 to generate a consensus sequence. Bases were called at positions spanned by at least two reads in each direction, where at least a 75% consensus on the allele was evident; additionally, the base quality at the site had to be at least 50, and the mapping quality had to be at least 30, on the Phred scale46,47. These consensus sequences from each representative of the sequence cluster were then combined to generate a reference-based multiple genome alignment, each of which was analysed as described previously using an earlier version of the Gubbins48 software. These fifteen whole genome alignments, which do not include the reference assemblies themselves, are also made available as part of Data Citation 2.

Code Availability

The algorithm used to predict recombination events has been developed into a software package, named Gubbins48, which can be installed on Linux and Max OSX operating systems. It can also be run on Windows operating systems using a virtual machine environment, and is freely available from http://sanger-pathogens.github.io/gubbins/. Code and reference sequences for the serological typing of S. pneumoniae using sequence reads is made available as part of Data Citation 2.

Data Records

The raw sequence data (FASTQ format) and annotated draft genome sequences (EMBL format) for the 616 isolates in the dataset have been deposited in the European Nucleotide Archive (http://www.ebi.ac.uk/ena/) with the project accession code in Data Citation 3. Individual accession codes for both raw read data and annotated draft genome assemblies are listed in Table 1 (available online only) and in the machine-readable ISA-tab metadata files associated with this article.

Further files are made available through the Dryad repository (https://datadryad.org) with the digital object identifier in Data Citation 2. The core genome codon alignment (FASTA format) and maximum likelihood phylogeny (Newick format) describe the overall population structure. For the study of individual lineages, a whole genome alignment (FASTA format) and draft reference assembly (FASTA format) are included for each of the 15 monophyletic sequence clusters19. These encompass 491 of the isolates, excluding those in the diverse polyphyletic sequence cluster 16. In addition, the full set of protein coding sequences (FASTA format), and the translated proteins (FASTA format), can be used to study the diversity of individual COGs. To minimise the manipulation of the protein sequences, the initial amino acid of each protein is directly translated, rather than being converted to a methionine.

The epidemiological and phylogenetic data can also be interactively visualised and analysed online using the Microreact website (http://microreact.org/) with the URL in Data Citation 4.

Technical Validation

Integrity of sample handling and quality control

An overview of the processing pipeline, including the technical validation steps, is shown in Fig. 1. Of the 716 samples collected as part of the surveillance project between 2001 and 2007, 631 could be revived and cultured. All these isolates were subjected to serological typing using latex agglutination to ensure consistency of sample handling relative to previous studies (Fig. 1 and Table 1 (available online only)). As multiple colonisation is often observed in children49 it was not necessarily expected that the original strain would be recovered in all cases; in the earlier studies, only a single isolate per individual was analysed in order to maximise the size of the host population sample, as detecting strains carried at low frequencies is inefficient using standard microbiological techniques50. The serology differed from that previously recorded for 12 of the 631 samples. These new serotype inferences were all independently verified as being correct using a second culture of the same isolate, and subsequently confirmed in all seven cases where the samples were retyped in a separate laboratory using the Quellung reaction51. One sample yielded two clearly morphologically distinct strains that proved to be of different serotypes, increasing the number of isolates in the study to 632. All isolates found to be non-typeable serologically were tested for optochin susceptibility using ‘P discs’; this identified one isolate as likely to represent a non-pneumococcal streptococcus, the exclusion of which reduced the overall collection size to 631.

Figure 1. Workflow for the generation of draft genome assemblies.

Figure 1

The flow chart shows the steps taken to generate the draft genome dataset; boxes in orange indicate steps at which typing was performed, allowing the integrity of sample handling to be checked, and boxes in blue indicate steps at which checks were performed to allow for the identification and elimination of low quality samples.

Sufficient high-quality genomic DNA for analysis was extracted from all isolates. After sequencing, nine samples failed either automated quality control checks implemented by the Sanger Institute, or manual investigation of dataset properties, such as adapter content, insert size and GC content; one of these failures was successfully resequenced. After assembly of the remaining 623 samples, seven further samples were rejected as generating low quality draft genomes. These represented cases where the N50 was below 15 kb and the total assembly length was greater than 2.4 Mb, likely as a consequence of sequence from more than one isolate being mixed in the raw Illumina reads. This resulted in the final dataset of 616 draft genomes.

Integrity of data handling

Serotypes and multilocus sequence types (STs) were inferred from Illumina read data as described previously23. Excluding isolates determined as being ‘non typeable’ by either microbiological or bioinformatic serotyping, across the final set of 616 samples the capsule polysaccharide synthesis (cps) locus was congruent with the serogroup (a set of antigenically similar serotypes) identified through immunological tests in all but two cases. Of the 594 isolates for which an ST had been previously established, 553 (93%) were identical with those inferred from Illumina reads (Fig. 2a). These included both cases where the genome’s cps locus did not match the experimentally ascertained serogroup, indicating these discrepancies were not likely to result from sampling handling issues. Of the 41 cases in which the original ST was discrepant with that inferred from the reads, 29 differed at only one of the seven loci (Fig. 2b). All remaining inconsistencies are likely to reflect instances of multiple colonisation, resulting in different strains being originally genotyped before storage and subsequently retrieved for sequencing. This is consistent with the cps locus from the sequence reads in these cases matching the serology of the revived isolates from which the genomic DNA was extracted.

Figure 2. Validation of the draft genome assemblies.

Figure 2

(a) Venn diagram showing the overlap between the sequence types from the original genotyping of the collection, those inferred from Illumina sequence read mapping, and those inferred from the genome assemblies. Only data for the 594 isolates for which all three datatypes were available are represented here. (b) Venn diagram showing the overlap between sequence types inferred by different methodologies, in this case treating results as being consistent if only one of the seven loci differed between results. In this case, the sequence types inferred from read mapping and de novo assembly are identical, and differ from the original genotyping in only twelve cases. (c) Histogram showing the number of CDSs in publicly available annotated complete, or high quality draft, S. pneumoniae genomes. (d) Histogram showing the number of CDSs in the 616 draft genomes from Massachusetts. This distribution shows that the count of putative CDSs in each draft genome is within the range of CDSs identified in manually annotated genomes, consistent with the draft genomes being near-complete, and the CDS predictions being accurate.

Quality of genome assemblies

The 616 samples in the dataset each yielded between 267 and 1,865 Mb of sequence data (median of 652 Mb). Assuming a typical 2 Mb S. pneumoniae genome26, this meant each isolate had a sequencing depth of over 100 fold coverage. Based on a random sample of 10,000 reads aligned to a set of prokaryotic and eukaryotic reference genomes using BWA52, a substantial majority of reads matched to the S. pneumoniae representative (strain ATCC 700669) in each dataset, confirming these data were primarily derived from the submitted genomic DNA sample.

All draft assemblies had an N50 greater than 15 kb and a total length between 1.98 and 2.19 Mb, similar to complete S. pneumoniae genomes. Additionally, the number of CDSs in the de novo assemblies was within the range of CDSs found within annotated complete or high-quality draft S. pneumoniae genomes (Fig. 2c,d). Each isolate’s annotation included at least 101 of the 102 protein functional domains recently suggested to be essential and ubiquitous across cellular genomes53; the only discrepancy was the short ribosomal protein coding sequence rpsN, which was not consistently identified by the automated gene annotation software even when present within assemblies. Assembly quality was also judged on the basis of non-coding RNA content: using previously-defined criteria53, the majority of isolates had a full-length representative of each of the ribosomal RNAs. Similarly, quantifying tRNA content found the majority of isolates had at least one tRNA for each standard amino acid.

To ascertain the accuracy of the de novo assemblies relative to the original epidemiological data and Illumina sequence reads, the STs were extracted from the contigs through identification of the relevant loci using BLAST54 (Fig. 2a,b). All seven loci could be recovered from each assembly. Across the 616 samples, the ST extracted from the assembly and Illumina reads was identical in 602 cases (98% accuracy). In four cases, the ST inferred from the assemblies differed from the consensus of the original genotyping and the ST extracted from the reads at a single locus (Fig. 2a). In six cases, the ST inferred from the Illumina reads differed from the consensus of the original genotyping and the assembly at a single locus; the assemblies indicated these all corresponded to a single ST, suggesting a rare systematic error. In four cases, the STs inferred from the assembly and Illumina reads differed at a single locus, and the original genotyping data were missing or inconsistent with both.

The wider ‘core’ genome was defined as a set of 1,194 COGs, a single representative of which was found in each genome19. Independent re-analysis of this dataset with a different method for defining COGs found 1,206 ‘core’ COGs, of which 1,027 were identical to the 1,194 originally identified55. Concatenated codon alignments of the ‘core’ COGs were subject to three independent analyses with BAPS version 5, which converged on identical membership of the sixteen sequence clusters, in two cases, with additional isolates included within SC7 in the third. The fifteen sequence clusters containing similar isolates were correspondingly monophyletic in the core genome phylogeny, confirming them as being closely related sets of bacteria (Fig. 3).

Figure 3. Overall population structure of the 616 S. pneumoniae isolates.

Figure 3

The maximum likelihood phylogeny generated from the core genome alignment is presented, as displayed in the Microreact system (Data Citation 4), with each leaf node coloured according to its sequence cluster (SC).

Validation of recombination analyses

Recombination detection was only attempted within the 15 monophyletic sequence clusters, as they consisted of groups of isolates with detectable similarity that was likely to reflect recent common ancestry. Simulations indicated that the type of approach that was used to identify recombinations in whole genome alignments is most accurate when applied to sets of closely-related sequences48. In the analyses presented in this work, all alignments in which at least ten recombination events were detected formed an exponential recombination length distribution with a rate constant consistent with other genomic data23,56–58 and experimental work47. The positions of recombination ‘hotspots’ relative to the reference genome annotations were also consistent with these independent analyses. In the cases where evidence of a molecular clock could be detected, the substitution rate was also found to be consistent with the analyses of other S. pneumoniae datasets23,56–58. Additionally, the consistency of the final phylogenies with the epidemiological data allowed a phylogeographic signal to be detected19.

Further analysis of the isolates’ inferred serology (Table 1 (available online only)) identified cases where closely-related isolates differed at their cps loci, suggesting ‘serotype switching’ had occurred. In all cases where the pattern of switching could be robustly established, the change at the cps locus could be attributed to an inferred recombination affecting the relevant genes4.

Usage Notes

Sequence data may be downloaded from the European Nucleotide Archive using the project accession codes ERP000809 or PRJEB2632. All accession codes for raw sequence data and annotated individual assemblies are listed in Table 1 (available online only). Associated epidemiological data was published previously19. Sequences and functional annotation can be displayed using Artemis41. Whole genome alignments can be viewed and analysed using standard software. Gubbins48 can be applied to them for the inference of recombined sequence. The software for serological typing can be run on Linux or Mac OSX as described in the accompanying README file.

Additional Information

Tables 1 is only available in the online version of this paper.

How to cite this article: Croucher, N. J. et al. Population genomic datasets describing the post-vaccine evolutionary epidemiology of Streptococcus pneumoniae. Sci. Data 2:150058 doi: 10.1038/sdata.2015.58 (2015).

Supplementary Material

sdata201558-isa1.zip (23.7KB, zip)

Acknowledgments

N.J.C. is funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and Royal Society (Grant Number 104169/Z/14/Z). Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases of the US National Institutes of Health (NIH) under award R01AI066304 and by Wellcome Trust grant 098051. We are grateful for the support of the Sanger Institute core sequencing and informatics teams.

Footnotes

S.I.P. has investigator-initiated grants from Merck and Pfizer and has consulted for GlaxoSmithKline, Merck, Pfizer and Novartis. W.P.H. has consulted for GlaxoSmithKline. M.L. has consulted for Pfizer and Novartis.

Data Citations

  1. Croucher N.J. 2009. International Nucleotide Sequence Database . FM211187
  2. Croucher N.J. 2015. Dryad. http://dx.doi.org/10.5061/dryad.t55gq
  3. Croucher N.J. 2013. International Nucleotide Sequence Database . PRJEB2632
  4. Croucher N.J. 2015. Microreact. http://microreact.org/project/NJwviE7F

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Citations

  1. Croucher N.J. 2009. International Nucleotide Sequence Database . FM211187
  2. Croucher N.J. 2015. Dryad. http://dx.doi.org/10.5061/dryad.t55gq
  3. Croucher N.J. 2013. International Nucleotide Sequence Database . PRJEB2632
  4. Croucher N.J. 2015. Microreact. http://microreact.org/project/NJwviE7F

Supplementary Materials

sdata201558-isa1.zip (23.7KB, zip)

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