Figure 5.

High viscosity-causing human IgG2κ mAb-(E)induces Russell body phenotypes. (A) Viscosity of mAb-E solution (closed circle) and mAb-C solution (open circle) was measured at 70, 95, 120, and 145 mg/ml in triplicate. Error bars denote standard deviation. Centipoise (cP) is a dynamic viscosity measurement unit. (B) Western blotting results for the cell culture media and cell lysates harvested on Day-7 post transfection. The expression constructs used for transfection are shown at the top of each lane. (C) Western blotting results on the cell culture media and cell lysates after 24 hr of BFA or null treatment from Day-2 to Day-3 post transfection. The cell viabilities after the 24 hr BFA and mock treatment were 85.2% and 89.8%, respectively. (D) Fluorescent micrographs of HEK293 cells co-transfected with HC and LC constructs. Cells at steady state are shown in the first row. Cells under BFA treatment condition are shown in the second row. Green, anti-gamma staining. Red, anti-kappa staining. (E and F) Fluorescent micrographs of HEK293 cells transfected with HC construct, shown in green (E) or with LC construct, shown in red (F). RBs are pointed by arrowheads in the bottom row.