Figure 1.

HDACs are deregulated in esophageal cancer cells. The panels show representative serial sections, respective the same tissue areas, of matching normal esophageal epithelium and ESCC or EAC tissue specimens stained for the epigenetic modifiers HDAC1, HDAC2, HDAC3, H3K9ac (red staining; via AP/Streptavidin, DAKO Real Detection system AP/RED) as well as DNMT1 and 5mC (brown staining, via DAB, DAKO envision FLEX+ Kit). Note reduced H3K9ac as well as loss of DNMT1 expression and 5mC (see arrows) in tumor cells of ESCCs and EACs as compared to normal epithelial cells (see inserts). Refer to Supplementary Data Table S1 for quantification of all cases. Bar represents 100 μm.