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. 2014 Jun 5;124(8):1335–1343. doi: 10.1182/blood-2014-01-552281

Figure 5.

Figure 5

HFE, but not HFE-C282Y and HFE-H63D, increases ALK3 cell-surface expression. Hep3B cells were transfected with ALK3-Flag in the presence or absence of HFE-HA (WT), HFE-HA (C282Y), and HFE-HA (H63D). Forty-eight hours after transfection, whole cells were incubated with and without Sulfo-NHS-LC-LC-Biotin, and the cell lysates were precipitated with Streptavidin Agarose. The whole-cell lysates and avidin precipitates were subjected to western blot analysis with antibodies as indicated. A nonspecific membrane protein was used as loading control. The experiment was repeated 3 times.