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10.1186/s12977-015-0215-z Nuclear accumulation of YFP-Vpr shed from post-fusion cytosolic HIV-1 cores. ASLVpp bearing the HIV-1 core label YFP-Vpr (green) and the content marker Gag-imCherry (red) were bound to CV-1/TVA950 cells in the cold. The cells were brought to a temperature-controlled microscope stage at 37 °C and entry was initiated by the addition of warm buffer. Images were collected every 4 s. Viral fusion, followed by nuclear accumulation of YFP-Vpr shed from post-fusion cytosolic cores, proceeded to completion in ~ 45 min (see also Figs. 3A and 7C and 7D in the main text).