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. 2015 May 29;6(20):18116–18133. doi: 10.18632/oncotarget.4016

Figure 2. 37LRP cDNA transfection in HEK-293 cells results in cell surface expression of the mature receptor and increased cell adhesion to LM.

Figure 2

A. HEK-293 cells were transfected with 37LRP cDNA fused at the C-terminal with a T7 tag and a poly-histidine stretch (37LRP/T7-tag), and named LR-293, or with the empty vector, V-293. Transfected cells were lysed and 50 μg of protein was analyzed by Western blot with 37LRP/67LR and T7-tag specific antibodies. B. Flow cytometric analysis of cell surface 67LR expression was evaluated by incubating V-293 and LR-293 cells with a polyclonal anti-67LR antibody or an isotype control. Fluorescence intensity values are reported. C. V-293 (□) and LR-293 (■) cells were plated on LM-coated wells in the presence of 20 μg/ml non immune immunoglobulins or anti-37LRP/67LR polyclonal antibodies. The attached cells were stained and the absorbance at 540 nm was measured. The values represent the mean ± SD of three experiments performed in triplicate. (*) p < 0.05, as determined by the Student's t test.