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. 2015 Jul 3;6(19):16939–16950. doi: 10.18632/oncotarget.4760

Figure 1. SUV39H2 methylates LSD1.

Figure 1

A. Recombinant LSD1 protein was methylated by SUV39H2 in a dose-dependent manner. An in vitro methyltransferase assay was performed by using purified His-tagged LSD1 and different amount of SUV39H2 recombinant proteins. Methylated LSD1 was detected by fluorography. Amounts of loading proteins were evaluated by staining with Ponceau S. B. Confirmation of the in vitro methyltransferase assay. Different amount of LSD1 protein was mixed with SUV39H2 in the presence of S-adenosyl-L-[methyl-3H]-methionine. Methylated LSD1 was detected by fluorography. Amounts of loading proteins were evaluated by staining with MemCodeTM Reversible Protein Stain (Thermo Fisher Scientific). C. The MS-MS spectrum corresponding to the trimethylated LSD1 322–347 peptide. The 42 Da increase of the Lys 322 residue was observed. D. MS chromatograms of unmodified and trimethylated LSD1 322–347 peptides.