Figure 5. Secretion of IFN-γ from DCs.

Secretion of IFN-γ from DCs after incubation with PECA cells treated by ALA-PDT with 630 nm LED light at doses of 0.5 J/cm² A. 1 J/cm² B. or 2 J/cm² C. at different times (0 to 24 h) after light irradiation, with untreated imDCs as a negative control. D. PECA cells were treated by ALA-PDT with different light doses (0.25 to 2 J/cm²). Six hours after treatment, PECA cells were collected and incubated with imDCs for 24 h and IFN-γ secreted from the DCs were quantified. E. PECA cells treated by PDT (0.5 mM ALA, 0.5 J/cm²) or three cycles of freeze thaw (F/T). Six hours after treatment, PECA cells were incubated with imDCs for 24 h, followed by measurement of IFN-γ secreted form DCs. Secretion of IFN-γ form untreated imDCs was used as a negative control and secretion of IFN-γ form DCs after incubation for 24 h with LPS was used as a positive control.