Figure 3. Global expression changes of sncdRNAs in normal and malignant prostate tissue.

Upper and middle panels present scatterplots comparing the normalized expression values of individual sncdRNA (dots) in each prostate cancer library (PCa) during progressing disease to these in the library prepared from normal adjacent prostate tissue (NAP). The expression of sncdRNAs in the hormone-refractory, transurethral resection of the prostate (TURP HR) library is also compared to the benign prostate hyperplasia (BPH) library since the latest represents the normal counterpart of malignant transurethral resection of the prostate material. Differences in sncdRNA expression between biological replicates of Gleason 6 cancers (PCa 6) as well as comparison of a fresh-frozen library (FF) with its formalin-fixed, paraffin-embedded (FFPE) counterpart derived from the same patients are presented in the lower panels. Each sncdRNA type is presented by a different color. Diagonal lines across each scatterplot represent fold change difference in expression. Middle line, crossing the horizontal and vertical axes at 0, no expression change; lines crossing the vertical and horizontal axes at 2, two-fold expression change; lines crossing the vertical and horizontal axes at 4, four-fold expression change. Cured, no disease relapse after radical prostatectomy; rec., recurrent disease, biochemical or metastatic relapse after surgery; LN, metastatic lymph node sample; TERG+, TMPRSS2-ERG fusion gene event; TERG-, no TMPRSS2-ERG fusion event; Numbers (6, 7 or 8) after PCa indicate the pathological Gleason score of the tumors in the respective group.