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. 2015 May 7;6(19):17584–17593. doi: 10.18632/oncotarget.3770

Figure 3. UXT-induced p53 inhibition caused activation of NF-B.

Figure 3

A. U2OS_UXT cells were subjected to Cignal Finder™ 10-Pathway Reporter Arrays (Qiagen). The results are from 3 independent experiments performed in triplicates. The numbers are means ± SD. B. U2OS_EV or U2OS_UXT cells were analyzed by immunostaining with an anti-p65 (Cell Signaling, MA) co-stained with DAPI. Representative images are shown. C. Cell lysates prepared from U2OS_EV or U2OS_UXT cells were analyzed by Immunoblot with the indicated antibodies. D. U2OS_EV or U2OS_UXT cells that were transfected with either siRNACtrl or siRNAp53 were treated with or without Nutlin-3a (10 μM 3 h). The cells were harvested and subjected Western analysis using the indicated antibodies.