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. 2015 Apr 22;6(19):17685–17697. doi: 10.18632/oncotarget.3779

Figure 1. TEAD4 interacts with KLF5 and suppresses the p27 gene expression in TNBC cell lines.

Figure 1

A. Protein expression levels of TEAD1–4 in breast epithelial cell lines by WB. β-actin serves as the loading control. B. TEAD4 specifically interacts with KLF5 in HEK293FT cells. All TEADs were tagged with Flag and immunoprecipitated with the anti-Flag antibody. Exogenous KLF5 was only immunoprecipitated by Flag-TEAD4. TEAD1-L and -S are two different TEAD1 isoforms. C. TEAD4 and KLF5 suppress the protein levels of p27 in both HCC1937 and HCC1806. TEAD4 and KLF5 were silenced by two different siRNAs in both cell lines. The p27 and β-actin protein levels were quantified by the IMAGE J software. The normalized p27 protein levels are shown below the p27 panel.