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. Author manuscript; available in PMC: 2015 Oct 30.
Published in final edited form as: Neuron. 2009 Jan 15;61(1):113–125. doi: 10.1016/j.neuron.2008.10.049

Figure 1. Induction but not expression of AD-ITM requires MAPK activity, and combined KCl depolarization and 5HT application activates MAPK.

Figure 1

A. The semi-intact preparation used to monitor T-SWR in the presence of pharmacological agents (shaded area). B1. The MEK inhibitor, U0126 (20µM; n=5) or its inactive analog, U0124 (20uM; n=6) were applied to the ring ganglia 1 hour before 1xTS and were present throughout the remainder of the experiment. B2. Summary data of 30–90 min time points combined. Within-group analysis revealed significant AD-ITM in controls, but not in groups treated with UO126, and a between group test revealed a significance difference. In this and subsequent figures, data are expressed as means ±SEM. C1. The MEK inhibitor, U0126 (20µM; n=6) or its inactive analog, U0124 (20uM; n=8) were applied to the ring ganglia immediately after 1xTS and were present throughout the remainder of the experiment. C2. Summary data in which intermediate time points 30–90 min were combined. Both groups exhibited significant AD-ITM; there was no significant difference between groups. D1. The treated pleural-pedal pair received i) a single pulse of 5HT (50µM, 5min; n=8), ii) a single pulse of high KCl ASW (100mM, 5 min; n=10), or iii) a combination of KCl and 5HT (KCl first, 2 min overlap with 5HT; n=11). Control side remained in ASW to establish a within-animal baseline (see Methods). Quantification of MAPK activation is represented as the ratio of phospho-MAPK (P-MAPK)/ total MAPK (MAPK) between the two sides (see Methods). D2. Summary data showing that only the combination of KCl+5HT produced significant MAPK activation. E. Pleural-Pedal pairs were divided and one side was stimulated with the analog (KCl+5HT). Sensory clusters were harvested at the indicated times. Within sample t-tests revealed that MAPK was significantly activated by the associative analog at each time point (p<0.05).