Skip to main content
NCBI home page
Thorax logoLink to Thorax
. 1990 Nov;45(11):856–859. doi: 10.1136/thx.45.11.856

Effect of frusemide on airway smooth muscle contractility in vitro.

A J Knox 1, P Ajao 1
PMCID: PMC462783  PMID: 2256014

Abstract

Frusemide, an inhibitor of sodium-potassium-chloride (Na-K-Cl) cotransport, has been shown to inhibit the airway response to several constrictor stimuli in asthmatic subjects. The protection seen with frusemide in these studies could be due to an effect on epithelium, inflammatory cells, neural pathways, or airway smooth muscle. To determine whether frusemide inhibits airway smooth muscle contraction, experiments were performed in bovine and human airways in vitro. Fresh bovine tissue was obtained from the abattoir and human tissue from thoracotomy. The effect of 10(-5)M frusemide on histamine, potassium chloride, and hyperosmolar saline induced contractions was studied in bovine tracheal strips without epithelium. Frusemide, at a concentration that specifically inhibits Na-K-Cl cotransport, did not inhibit contraction caused by any of these agents. Frusemide was also without effect on hypertonic saline induced contractions of bovine and human bronchial rings with epithelium intact. These results suggest that modification of Na-K-Cl cotransport does not alter airway smooth muscle contractility and that the protective effect of frusemide on induced bronchoconstriction in vivo is unlikely to be due to a direct effect on airway smooth muscle.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Advenier C., Cerrina J., Duroux P., Floch A., Renier A. Effects of five different organic calcium antagonists on guinea-pig isolated trachea. Br J Pharmacol. 1984 Jul;82(3):727–733. doi: 10.1111/j.1476-5381.1984.tb10812.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bianco S., Pieroni M. G., Refini R. M., Rottoli L., Sestini P. Protective effect of inhaled furosemide on allergen-induced early and late asthmatic reactions. N Engl J Med. 1989 Oct 19;321(16):1069–1073. doi: 10.1056/NEJM198910193211602. [DOI] [PubMed] [Google Scholar]
  3. Bianco S., Vaghi A., Robuschi M., Pasargiklian M. Prevention of exercise-induced bronchoconstriction by inhaled frusemide. Lancet. 1988 Jul 30;2(8605):252–255. doi: 10.1016/s0140-6736(88)92540-8. [DOI] [PubMed] [Google Scholar]
  4. Chapman H. A., Jr, Reilly J. J., Jr, Kobzik L. Role of plasminogen activator in degradation of extracellular matrix protein by live human alveolar macrophages. Am Rev Respir Dis. 1988 Feb;137(2):412–419. doi: 10.1164/ajrccm/137.2.412. [DOI] [PubMed] [Google Scholar]
  5. Chipperfield A. R. The (Na+-K+-Cl-) co-transport system. Clin Sci (Lond) 1986 Nov;71(5):465–476. doi: 10.1042/cs0710465. [DOI] [PubMed] [Google Scholar]
  6. Deth R. C., Payne R. A., Peecher D. M. Influence of furosemide on rubidium-86 uptake and alpha-adrenergic responsiveness of arterial smooth muscle. Blood Vessels. 1987;24(6):321–333. doi: 10.1159/000158709. [DOI] [PubMed] [Google Scholar]
  7. Hall I. P., Chilvers E. R. Inositol phosphates and airway smooth muscle. Pulm Pharmacol. 1989;2(3):113–120. doi: 10.1016/0952-0600(89)90034-3. [DOI] [PubMed] [Google Scholar]
  8. Lundergan C. F., Fitzpatrick T. M., Rose J. C., Ramwell P. W., Kot P. A. Effect of cyclooxygenase inhibition on the pulmonary vasodilator response to furosemide. J Pharmacol Exp Ther. 1988 Jul;246(1):102–106. [PubMed] [Google Scholar]
  9. Rasmussen H., Takuwa Y., Park S. Protein kinase C in the regulation of smooth muscle contraction. FASEB J. 1987 Sep;1(3):177–185. [PubMed] [Google Scholar]
  10. Van Breemen C., Aaronson P., Loutzenhiser R. Sodium-calcium interactions in mammalian smooth muscle. Pharmacol Rev. 1978 Jun;30(2):167–208. [PubMed] [Google Scholar]
  11. Welsh M. J. Inhibition of chloride secretion by furosemide in canine tracheal epithelium. J Membr Biol. 1983;71(3):219–226. doi: 10.1007/BF01875463. [DOI] [PubMed] [Google Scholar]
  12. Widdicombe J. H., Nathanson I. T., Highland E. Effects of "loop" diuretics on ion transport by dog tracheal epithelium. Am J Physiol. 1983 Nov;245(5 Pt 1):C388–C396. doi: 10.1152/ajpcell.1983.245.5.C388. [DOI] [PubMed] [Google Scholar]
  13. van Breemen C., Saida K. Cellular mechanisms regulating [Ca2+]i smooth muscle. Annu Rev Physiol. 1989;51:315–329. doi: 10.1146/annurev.ph.51.030189.001531. [DOI] [PubMed] [Google Scholar]

Articles from Thorax are provided here courtesy of BMJ Publishing Group

RESOURCES