Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Apr 15;90(8):3314–3318. doi: 10.1073/pnas.90.8.3314

Data base analysis of protein expression patterns during T-cell ontogeny and activation.

S M Hanash 1, J R Strahler 1, Y Chan 1, R Kuick 1, D Teichroew 1, J V Neel 1, N Hailat 1, D R Keim 1, J Gratiot-Deans 1, D Ungar 1, et al.
PMCID: PMC46290  PMID: 8475073

Abstract

We have developed a data base of lymphoid proteins detectable by two-dimensional polyacrylamide gel electrophoresis. The data base contains two-dimensional patterns and derived information pertaining to polypeptide constituents of unstimulated and stimulated mature T cells and immature thymocytes, single-cell-derived T- and B-cell clones, leukemia cells, and lymphoid cell lines. Using this data base, we have compared the protein constituents of mature T cells and immature thymocytes before and after mitotic stimulation. A subset of polypeptides that are induced in mature T cells following mitotic stimulation were found to be constitutively expressed in immature thymocytes. Other polypeptides exhibited differences in their expression between mature and immature thymocytes in a manner unrelated to proliferation. The identity of several constitutively expressed or mitotically induced proteins in lymphoid cells was established by microsequencing. These initial findings point to significant differences in the molecular pathways leading to proliferation between mature and immature T cells. The construction of this database should facilitate further studies of lymphoid differentiation and function.

Full text

PDF
3314

Images in this article

3316Fig. 1
on p.3316
3317Fig. 2
on p.3317
3317Fig. 3
on p.3317

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali I., Chan Y., Kuick R., Teichroew D., Hanash S. M. Implementation of a two-dimensional electrophoresis-related laboratory information processing system: database aspects. Electrophoresis. 1991 Oct;12(10):747–761. doi: 10.1002/elps.1150121008. [DOI] [PubMed] [Google Scholar]
  2. Chan P. K., Aldrich M., Cook R. G., Busch H. Amino acid sequence of protein B23 phosphorylation site. J Biol Chem. 1986 Feb 5;261(4):1868–1872. [PubMed] [Google Scholar]
  3. Crabtree G. R. Contingent genetic regulatory events in T lymphocyte activation. Science. 1989 Jan 20;243(4889):355–361. doi: 10.1126/science.2783497. [DOI] [PubMed] [Google Scholar]
  4. Feuerstein N. Phosphorylation of numatrin and other nuclear proteins by cdc2 containing CTD kinase cdc2/p58. J Biol Chem. 1991 Aug 25;266(24):16200–16206. [PubMed] [Google Scholar]
  5. Gilles A. M., Presecan E., Vonica A., Lascu I. Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme. J Biol Chem. 1991 May 15;266(14):8784–8789. [PubMed] [Google Scholar]
  6. Gratiot-Deans J., Keim D., Strahler J. R., Turka L. A., Hanash S. Differential expression of Op18 phosphoprotein during human thymocyte maturation. J Clin Invest. 1992 Oct;90(4):1576–1581. doi: 10.1172/JCI116026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gross B., Gaestel M., Böhm H., Bielka H. cDNA sequence coding for a translationally controlled human tumor protein. Nucleic Acids Res. 1989 Oct 25;17(20):8367–8367. doi: 10.1093/nar/17.20.8367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hailat N., Keim D. R., Melhem R. F., Zhu X. X., Eckerskorn C., Brodeur G. M., Reynolds C. P., Seeger R. C., Lottspeich F., Strahler J. R. High levels of p19/nm23 protein in neuroblastoma are associated with advanced stage disease and with N-myc gene amplification. J Clin Invest. 1991 Jul;88(1):341–345. doi: 10.1172/JCI115299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanash S. M., Strahler J. R., Kuick R., Chu E. H., Nichols D. Identification of a polypeptide associated with the malignant phenotype in acute leukemia. J Biol Chem. 1988 Sep 15;263(26):12813–12815. [PubMed] [Google Scholar]
  10. Hanash S. M., Strahler J. R., Neel J. V., Hailat N., Melhem R., Keim D., Zhu X. X., Wagner D., Gage D. A., Watson J. T. Highly resolving two-dimensional gels for protein sequencing. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5709–5713. doi: 10.1073/pnas.88.13.5709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Keim D., Hailat N., Hodge D., Hanash S. M. Proliferating cell nuclear antigen expression in childhood acute leukemia. Blood. 1990 Sep 1;76(5):985–990. [PubMed] [Google Scholar]
  12. Keim D., Hailat N., Melhem R., Zhu X. X., Lascu I., Veron M., Strahler J., Hanash S. M. Proliferation-related expression of p19/nm23 nucleoside diphosphate kinase. J Clin Invest. 1992 Mar;89(3):919–924. doi: 10.1172/JCI115672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krainer A. R., Mayeda A., Kozak D., Binns G. Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell. 1991 Jul 26;66(2):383–394. doi: 10.1016/0092-8674(91)90627-b. [DOI] [PubMed] [Google Scholar]
  14. Landry J., Lambert H., Zhou M., Lavoie J. N., Hickey E., Weber L. A., Anderson C. W. Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II. J Biol Chem. 1992 Jan 15;267(2):794–803. [PubMed] [Google Scholar]
  15. Lieu T. S., Newkirk M. M., Capra J. D., Sontheimer R. D. Molecular characterization of human Ro/SS-A antigen. Amino terminal sequence of the protein moiety of human Ro/SS-A antigen and immunological activity of a corresponding synthetic peptide. J Clin Invest. 1988 Jul;82(1):96–101. doi: 10.1172/JCI113607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McIntosh E. M., Ager D. D., Gadsden M. H., Haynes R. H. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8020–8024. doi: 10.1073/pnas.89.17.8020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Park M. H., Chung S. I., Cooper H. L., Folk J. E. The mammalian hypusine-containing protein, eukaryotic initiation factor 4D. Structural homology of this protein from several species. J Biol Chem. 1984 Apr 10;259(7):4563–4565. [PubMed] [Google Scholar]
  18. Pihlajaniemi T., Helaakoski T., Tasanen K., Myllylä R., Huhtala M. L., Koivu J., Kivirikko K. I. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987 Mar;6(3):643–649. doi: 10.1002/j.1460-2075.1987.tb04803.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  20. Sakai K., Hirai M., Minoshima S., Kudoh J., Fukuyama R., Shimizu N. Isolation of cDNAs encoding a substrate for protein kinase C: nucleotide sequence and chromosomal mapping of the gene for a human 80K protein. Genomics. 1989 Aug;5(2):309–315. doi: 10.1016/0888-7543(89)90063-3. [DOI] [PubMed] [Google Scholar]
  21. Strahler J. R., Kuick R., Eckerskorn C., Lottspeich F., Richardson B. C., Fox D. A., Stoolman L. M., Hanson C. A., Nichols D., Tueche H. J. Identification of two related markers for common acute lymphoblastic leukemia as heat shock proteins. J Clin Invest. 1990 Jan;85(1):200–207. doi: 10.1172/JCI114413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strahler J. R., Lamb B. J., Ungar D. R., Fox D. A., Hanash S. M. Cell cycle progression is associated with distinct patterns of phosphorylation of Op18. Biochem Biophys Res Commun. 1992 May 29;185(1):197–203. doi: 10.1016/s0006-291x(05)80975-1. [DOI] [PubMed] [Google Scholar]
  23. Ting J., Lee A. S. Human gene encoding the 78,000-dalton glucose-regulated protein and its pseudogene: structure, conservation, and regulation. DNA. 1988 May;7(4):275–286. doi: 10.1089/dna.1988.7.275. [DOI] [PubMed] [Google Scholar]
  24. Turka L. A., Linsley P. S., Paine R., 3rd, Schieven G. L., Thompson G. B., Ledbetter J. A. Signal transduction via CD4, CD8, and CD28 in mature and immature thymocytes. Implications for thymic selection. J Immunol. 1991 Mar 1;146(5):1428–1436. [PubMed] [Google Scholar]
  25. von der Kammer H., Klaudiny J., Zimmer M., Scheit K. H. Human elongation factor 1 beta: cDNA and derived amino acid sequence. Biochem Biophys Res Commun. 1991 May 31;177(1):312–317. doi: 10.1016/0006-291x(91)91984-k. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES