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. 1993 Apr 15;90(8):3378–3382. doi: 10.1073/pnas.90.8.3378

The two motility systems of Myxococcus xanthus show different selective advantages on various surfaces.

W Shi 1, D R Zusman 1
PMCID: PMC46303  PMID: 8475084

Abstract

Myxococcus xanthus, a bacterium that forms fruiting bodies, moves by gliding motility utilizing dual motility systems that differ both genetically and morphologically [system A, having at least 21 genetic loci and moving mainly single cells, and system S, having at least 10 genetic loci and moving groups (rafts) of cells] [Hodgkin, J. & Kaiser, D. (1979) Mol. Gen. Genet. 172, 177-191]. In this study, we found that A- and S-gliding-motility systems have different selective advantages on surfaces containing different concentrations of agar. We observed that colonies of A+S- cells (A-motile cells) swarmed better than A-S+ cells (S-motile cells) on relatively firm and dry surfaces (e.g., 1.5% agar). In contrast, colonies of A-S+ cells swarmed much better than A+S- cells on soft and wet surfaces (e.g., 0.3% agar). Individual A-motile cells moved at a rate of 2-4 microns/min on 1.5% agar but they barely moved on 0.3% agar (< 0.5 microns/min); in contrast S-motile cells moved 3-5 times faster on 0.3% agar than on 1.5% agar. Wild-type cells with both A- and S-motility systems were able to move well over a wide range of surfaces. These results suggest that dual motility systems enable the myxobacteria to adapt to a variety of physiological and ecological environments and show similarities in function to the dual motility systems of flagellated bacteria such as Vibrio spp.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumann P., Baumann L. Biology of the marine enterobacteria: genera Beneckea and Photobacterium. Annu Rev Microbiol. 1977;31:39–61. doi: 10.1146/annurev.mi.31.100177.000351. [DOI] [PubMed] [Google Scholar]
  2. Behmlander R. M., Dworkin M. Extracellular fibrils and contact-mediated cell interactions in Myxococcus xanthus. J Bacteriol. 1991 Dec;173(24):7810–7820. doi: 10.1128/jb.173.24.7810-7820.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Belas R., Mileham A., Simon M., Silverman M. Transposon mutagenesis of marine Vibrio spp. J Bacteriol. 1984 Jun;158(3):890–896. doi: 10.1128/jb.158.3.890-896.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burchard R. P. Gliding motility mutants of Myxococcus xanthus. J Bacteriol. 1970 Nov;104(2):940–947. doi: 10.1128/jb.104.2.940-947.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Campos J. M., Geisselsoder J., Zusman D. R. Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978 Feb 25;119(2):167–178. doi: 10.1016/0022-2836(78)90431-x. [DOI] [PubMed] [Google Scholar]
  6. Campos J. M., Zusman D. R. Regulation of development in Myxococcus xanthus: effect of 3':5'-cyclic AMP, ADP, and nutrition. Proc Natl Acad Sci U S A. 1975 Feb;72(2):518–522. doi: 10.1073/pnas.72.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fink J. M., Zissler J. F. Characterization of lipopolysaccharide from Myxococcus xanthus by use of monoclonal antibodies. J Bacteriol. 1989 Apr;171(4):2028–2032. doi: 10.1128/jb.171.4.2028-2032.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOUWINK A. L., van ITERSON W. Electron microscopical observations on bacterial cytology; a study on flagellation. Biochim Biophys Acta. 1950 Mar;5(1):10–44. doi: 10.1016/0006-3002(50)90144-2. [DOI] [PubMed] [Google Scholar]
  9. Hagen D. C., Bretscher A. P., Kaiser D. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev Biol. 1978 Jun;64(2):284–296. doi: 10.1016/0012-1606(78)90079-9. [DOI] [PubMed] [Google Scholar]
  10. Hodgkin J., Kaiser D. Cell-to-cell stimulation of movement in nonmotile mutants of Myxococcus. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2938–2942. doi: 10.1073/pnas.74.7.2938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaiser D. Social gliding is correlated with the presence of pili in Myxococcus xanthus. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5952–5956. doi: 10.1073/pnas.76.11.5952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEIFSON E., HUGH R. Variation in shape and arrangement of bacterial flagella. J Bacteriol. 1953 Mar;65(3):263–271. doi: 10.1128/jb.65.3.263-271.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MacRae T. H., Dobson W. J., McCurdy H. D. Fimbriation in gliding bacteria. Can J Microbiol. 1977 Aug;23(8):1096–1108. doi: 10.1139/m77-165. [DOI] [PubMed] [Google Scholar]
  14. McCarter L., Hilmen M., Silverman M. Flagellar dynamometer controls swarmer cell differentiation of V. parahaemolyticus. Cell. 1988 Jul 29;54(3):345–351. doi: 10.1016/0092-8674(88)90197-3. [DOI] [PubMed] [Google Scholar]
  15. Sar N., McCarter L., Simon M., Silverman M. Chemotactic control of the two flagellar systems of Vibrio parahaemolyticus. J Bacteriol. 1990 Jan;172(1):334–341. doi: 10.1128/jb.172.1.334-341.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shimkets L. J. Role of cell cohesion in Myxococcus xanthus fruiting body formation. J Bacteriol. 1986 Jun;166(3):842–848. doi: 10.1128/jb.166.3.842-848.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shimkets L. J. Social and developmental biology of the myxobacteria. Microbiol Rev. 1990 Dec;54(4):473–501. doi: 10.1128/mr.54.4.473-501.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shinoda S., Okamoto K. Formation and function of Vibrio parahaemolyticus lateral flagella. J Bacteriol. 1977 Mar;129(3):1266–1271. doi: 10.1128/jb.129.3.1266-1271.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stephens K., Hartzell P., Kaiser D. Gliding motility in Myxococcus xanthus: mgl locus, RNA, and predicted protein products. J Bacteriol. 1989 Feb;171(2):819–830. doi: 10.1128/jb.171.2.819-830.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ulitzer S. The mechanism of swarming of Vibrio alginolyticus. Arch Microbiol. 1975 Jun 20;104(1):67–71. doi: 10.1007/BF00447301. [DOI] [PubMed] [Google Scholar]
  21. Zusman D. R., McBride M. J. Sensory transduction in the gliding bacterium Myxococcus xanthus. Mol Microbiol. 1991 Oct;5(10):2323–2329. doi: 10.1111/j.1365-2958.1991.tb02077.x. [DOI] [PubMed] [Google Scholar]

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