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. 1993 Apr 15;90(8):3408–3412. doi: 10.1073/pnas.90.8.3408

Mapping cis-acting defects in promoters of transcriptionally silent DQA2, DQB2, and DOB genes.

C F Voliva 1, S Tsang 1, B M Peterlin 1
PMCID: PMC46309  PMID: 8475089

Abstract

Defects in promoters of the nonexpressed DQA2, DQB2, and DOB genes from the class II major histo-compatibility complex were mapped by placing Z and X boxes of these silent genes into a synthetic DRA promoter. These conserved upstream sequences confer B-cell-specific and gamma-interferon-inducible expression to the DRA gene. Since DRA promoters containing the X box from the DQA2 gene and Z boxes from DQA2, DQB2, and DOB genes were neither expressed constitutively in B cells nor inducible by gamma interferon in fibroblastic cells, these conserved upstream sequences are implicated in the transcriptional defects of these silent genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson G., Larhammar D., Widmark E., Servenius B., Peterson P. A., Rask L. Class II genes of the human major histocompatibility complex. Organization and evolutionary relationship of the DR beta genes. J Biol Chem. 1987 Jun 25;262(18):8748–8758. [PubMed] [Google Scholar]
  2. Andersson G., Peterlin B. M. NF-X2 that binds to the DRA X2-box is activator protein 1. Expression cloning of c-Jun. J Immunol. 1990 Nov 15;145(10):3456–3462. [PubMed] [Google Scholar]
  3. Auffray C., Lillie J. W., Korman A. J., Boss J. M., Fréchin N., Guillemot F., Cooper J., Mulligan R. C., Strominger J. L. Structure and expression of HLA-DQ alpha and -DX alpha genes: interallelic alternate splicing of the HLA-DQ alpha gene and functional splicing of the HLA-DQ alpha gene using a retroviral vector. Immunogenetics. 1987;26(1-2):63–73. doi: 10.1007/BF00345456. [DOI] [PubMed] [Google Scholar]
  4. Benoist C., Mathis D. Regulation of major histocompatibility complex class-II genes: X, Y and other letters of the alphabet. Annu Rev Immunol. 1990;8:681–715. doi: 10.1146/annurev.iy.08.040190.003341. [DOI] [PubMed] [Google Scholar]
  5. Berdoz J., Tiercy J. M., Rollini P., Mach B., Gorski J. Remarkable sequence conservation of the HLA-DQB2 locus (DX beta) within the highly polymorphic DQ subregion of the human MHC. Immunogenetics. 1989;29(4):241–248. doi: 10.1007/BF00717908. [DOI] [PubMed] [Google Scholar]
  6. Boothby M., Liou H. C., Finn P. W., Gravallese E., Glimcher L. H. Control of transcription at the murine A alpha locus. Immunol Res. 1990;9(2):77–92. doi: 10.1007/BF02918199. [DOI] [PubMed] [Google Scholar]
  7. Boss J. M., Strominger J. L. Regulation of a transfected human class II major histocompatibility complex gene in human fibroblasts. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9139–9143. doi: 10.1073/pnas.83.23.9139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Das H. K., Lawrance S. K., Weissman S. M. Structure and nucleotide sequence of the heavy chain gene of HLA-DR. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3543–3547. doi: 10.1073/pnas.80.12.3543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gaur L. K., Heise E. R., Thurtle P. S., Nepom G. T. Conservation of the HLA-DQB2 locus in nonhuman primates. J Immunol. 1992 Feb 1;148(3):943–948. [PubMed] [Google Scholar]
  10. Hooft van Huijsduijnen R., Li X. Y., Black D., Matthes H., Benoist C., Mathis D. Co-evolution from yeast to mouse: cDNA cloning of the two NF-Y (CP-1/CBF) subunits. EMBO J. 1990 Oct;9(10):3119–3127. doi: 10.1002/j.1460-2075.1990.tb07509.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hughes A. L., Nei M. Evolutionary relationships of class II major-histocompatibility-complex genes in mammals. Mol Biol Evol. 1990 Nov;7(6):491–514. doi: 10.1093/oxfordjournals.molbev.a040622. [DOI] [PubMed] [Google Scholar]
  12. Jonsson A. K., Hyldig-Nielsen J. J., Servenius B., Larhammar D., Andersson G., Jörgensen F., Peterson P. A., Rask L. Class II genes of the human major histocompatibility complex. Comparisons of the DQ and DX alpha and beta genes. J Biol Chem. 1987 Jun 25;262(18):8767–8777. [PubMed] [Google Scholar]
  13. Kappes D., Strominger J. L. Human class II major histocompatibility complex genes and proteins. Annu Rev Biochem. 1988;57:991–1028. doi: 10.1146/annurev.bi.57.070188.005015. [DOI] [PubMed] [Google Scholar]
  14. Kouskoff V., Mantovani R. M., Candéias S. M., Dorn A., Staub A., Lisowska-Grospierre B., Griscelli C., Benoist C. O., Mathis D. J. NF-X, a transcription factor implicated in MHC class II gene regulation. J Immunol. 1991 May 1;146(9):3197–3204. [PubMed] [Google Scholar]
  15. Larhammar D., Servenius B., Rask L., Peterson P. A. Characterization of an HLA DR beta pseudogene. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1475–1479. doi: 10.1073/pnas.82.5.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Liou H. C., Boothby M. R., Finn P. W., Davidon R., Nabavi N., Zeleznik-Le N. J., Ting J. P., Glimcher L. H. A new member of the leucine zipper class of proteins that binds to the HLA DR alpha promoter. Science. 1990 Mar 30;247(4950):1581–1584. doi: 10.1126/science.2321018. [DOI] [PubMed] [Google Scholar]
  17. Ono S. J., Bazil V., Levi B. Z., Ozato K., Strominger J. L. Transcription of a subset of human class II major histocompatibility complex genes is regulated by a nucleoprotein complex that contains c-fos or an antigenically related protein. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4304–4308. doi: 10.1073/pnas.88.10.4304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ono S. J., Liou H. C., Davidon R., Strominger J. L., Glimcher L. H. Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4309–4312. doi: 10.1073/pnas.88.10.4309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Osborn L., Kunkel S., Nabel G. J. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2336–2340. doi: 10.1073/pnas.86.7.2336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reith W., Barras E., Satola S., Kobr M., Reinhart D., Sanchez C. H., Mach B. Cloning of the major histocompatibility complex class II promoter binding protein affected in a hereditary defect in class II gene regulation. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4200–4204. doi: 10.1073/pnas.86.11.4200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Servenius B., Rask L., Peterson P. A. Class II genes of the human major histocompatibility complex. The DO beta gene is a divergent member of the class II beta gene family. J Biol Chem. 1987 Jun 25;262(18):8759–8766. [PubMed] [Google Scholar]
  22. Shewey L. M., Beaty J. S., Andersen L. C., Nepom G. T. Differential expression of related HLA class II DQ beta genes caused by nucleotide variation in transcriptional regulatory elements. J Immunol. 1992 Feb 15;148(4):1265–1273. [PubMed] [Google Scholar]
  23. Tonnelle C., DeMars R., Long E. O. DO beta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 1985 Nov;4(11):2839–2847. doi: 10.1002/j.1460-2075.1985.tb04012.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tsang S. Y., Nakanishi M., Peterlin B. M. B-cell-specific and interferon-gamma-inducible regulation of the HLA-DR alpha gene. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8598–8602. doi: 10.1073/pnas.85.22.8598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tsang S. Y., Nakanishi M., Peterlin B. M. Mutational analysis of the DRA promoter: cis-acting sequences and trans-acting factors. Mol Cell Biol. 1990 Feb;10(2):711–719. doi: 10.1128/mcb.10.2.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Viville S., Jongeneel V., Koch W., Mantovani R., Benoist C., Mathis D. The E alpha promoter: a linker-scanning analysis. J Immunol. 1991 May 1;146(9):3211–3217. [PubMed] [Google Scholar]
  27. Voliva C. F., Aronheim A., Walker M. D., Peterlin B. M. B-cell factor 1 is required for optimal expression of the DRA promoter in B cells. Mol Cell Biol. 1992 May;12(5):2383–2390. doi: 10.1128/mcb.12.5.2383. [DOI] [PMC free article] [PubMed] [Google Scholar]

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