There is an error in the fifth sentence of the Abstract. The correct sentence is: Furthermore we report, for the first time, the identification of the ace.1 G119S mutation in An. arabiensis populations collected at 8 sites.
There is also an error in the first sentence of the Discussion section. The correct sentence is: This study provides current information on the distribution of three members of the Anopheles gambiae complex across Burkina Faso and the frequency and distribution of three important target-site resistance mechanisms in these populations.
In Tables 2 and 3, the values in the column [95% CI] are incorrect. Please view the corrected Tables 2 and 3 here.
Table 2. Allelic and genotypic frequencies at the kdr 1014F and 1014S locus in An. gambiae s.l populations.
| Species | Sites | N | Genotypes | Genotypes | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1014L | 1014L | 1014F | f(L1014F) | [95%Cl] | p(HW) | 1014L | 1014L | f(L1014F) | [95%Cl] | p(HW) | |||
| 1014L | 1014F | 1014F | 1014L | 1014F | |||||||||
| An. arabiensis | Gaoua | 5 | 1 | 0 | 2 | 0.66 | [0.24–1] | - | 0 | 2 | 0.66 | [0.24–1] | 0.2000 |
| Banfora | 0 | 0 | 0 | 0 | - | - | - | 0 | 0 | 0 | - | - | |
| Sindou | 10 | 5 | 0 | 0 | 0 | - | - | 1 | 4 | 0.9 | [0.71–1] | - | |
| Orodara | 1 | 1 | 0 | 0 | 0 | - | 0.4678 | 0 | 0 | 0 | - | - | |
| Dioulassoba | 30 | 1 | 5 | 14 | 0.82 | [0.68–0.95] | 0.2308 | 2 | 8 | 0.42 | [0.24–0.59] | 0.0003 | |
| Soumousso | 11 | 1 | 1 | 5 | 0.78 | [0.53–1] | 0.0956 | 2 | 2 | 0.37 | [0.08–0.65] | 0.2914 | |
| Boromo | 17 | 2 | 3 | 12 | 0.79 | [0.59–0.98] | 0.000 | 6 | 3 | 0.28 | [0.06–0.49] | 0.3405 | |
| Dédougou | 23 | 6 | 0 | 10 | 0.62 | [0.42–0.81] | 0.1652 | 5 | 2 | 0 | - | 0.3213 | |
| Koudougou | 13 | 2 | 3 | 8 | 0.73 | [0.48–0.97] | - | 0 | 0 | 0.5 | [0.22–0.77] | - | |
| Nanoro | 6 | 4 | 0 | 0 | 0 | - | 0.4406 | 0 | 2 | 0.5 | [0.09–0.90] | 0.0857 | |
| Koupela | 13 | 2 | 3 | 3 | 0.56 | [0.29–0.82] | 0.2970 | 2 | 3 | 0.53 | [0.25–0.80] | 0.1795 | |
| Fada | 25 | 6 | 5 | 3 | 0.39 | [0.19–0.58] | 0.0933 | 8 | 3 | 0.57 | [0.37–0.76] | 0.9035 | |
| Kaya | 17 | 4 | 3 | 5 | 0.54 | [0.30–0.77] | - | 1 | 4 | 0.37 | [0.14–0.59] | 0.0061 | |
| Ouahigouya | 1 | 0 | 1 | 0 | 0.5 | [0–1] | 0.0031 | 0 | 0 | 0 | - | - | |
| Dori | 22 | 6 | 2 | 8 | 0.56 | 0.35–0.76] | - | 4 | 2 | 0.26 | [0.07–0.44] | 0.2260 | |
| An. coluzzii | Gaoua | 1 | 1 | 0 | 0 | 0 | - | - | 0 | 0 | 0 | - | - |
| Banfora | 7 | 0 | 1 | 5 | 0.91 | [6.69–1] | - | 0 | 1 | 0.16 | [0–0.43] | 0.0909 | |
| Sindou | 12 | 1 | 1 | 4 | 0.75 | [0.50–0.99] | 0.2727 | 1 | 5 | 0.91 | [0.74–1 | - | |
| Orodara | 5 | 2 | 1 | 1 | 0.37 | [0–0.79] | 0.4286 | 1 | 0 | 0.12 | [0–0.40] | - | |
| Dioulassoba | 9 | 1 | 1 | 3 | 0.7 | [0.40–0.99] | 0.3333 | 1 | 3 | 0.7 | [0.40–0.99] | 0.3333 | |
| Soumousso | 4 | 2 | 1 | 0 | 0.16 | [0–0.51] | - | 0 | 1 | 0.33 | [0–0.79] | 0.2000 | |
| Boromo | 0 | 0 | 0 | 0 | - | - | - | 0 | 0 | - | - | - | |
| Dédougou | 3 | 1 | 1 | 0 | 0.25 | [0–0.74] | - | 0 | 1 | 0.5 | [0–1] | 0.6190 | |
| Koudougou | 7 | 0 | 3 | 2 | 0.7 | |0.36–1] | 1 | 2 | 0 | 0.2 | [0–0.5] | - | |
| Nanoro | 39 | 1 | 5 | 18 | 0.85 | [0.73–0.96] | 0.3983 | 12 | 3 | 0.37 | [0.21–0.52] | 0.3333 | |
| Koupela | 9 | 3 | 5 | 0 | 0.31 | |0–0.61] | 1 | 1 | 0 | 0.06 | [0–0.21] | 0.7446 | |
| Fada | 46 | 7 | 7 | 13 | 0.61 | [0.46–0.75] | 0.0186 | 17 | 2 | 0.38 | [0.23–0.52] | 0.0817 | |
| Kaya | 8 | 2 | 0 | 3 | 0.6 | [0.26–0.93] | 0.0476 | 2 | 1 | 0.4 | [0.06–0.73] | 0.3333 | |
| Ouahigouya | 17 | 4 | 0 | 6 | 0.6 | [0.36–0.83 | 0.0017 | 2 | 5 | 0.6 | [0.36–0.83] | 1 | |
| Dori | 9 | 3 | 0 | 2 | 0.4 | [0.07–0.72] | 0.0476 | 1 | 3 | 0.7 | [0.40–0.99] | - | |
| An. gambiae | Gaoua | 74 | 14 | 8 | 17 | 0.53 | [0.41–0.64] | 0.0002 | 0 | 35 | 0.92 | [0.85–0.98] | 1 |
| Banfora | 29 | 7 | 7 | 10 | 0.56 | [0.37–0.74] | 0.0434 | 3 | 2 | 0.14 | [0.01–0.26] | 0.1518 | |
| Sindou | 46 | 8 | 3 | 13 | 0.6 | [0.45–0.74] | 0.0003 | 5 | 17 | 0.81 | [0.69–0.92] | 0.0611 | |
| Orodara | 33 | 5 | 7 | 11 | 0.63 | [0.46–0.79] | 0.0904 | 1 | 9 | 0.41 | [0.24–0.57] | 0.0420 | |
| Dioulassoba | 8 | 0 | 1 | 3 | 0.87 | [0.63–1] | - | 2 | 2 | 0.75 | [0.44–1] | 0.3257 | |
| Soumousso | 29 | 8 | 9 | 3 | 0.37 | [0.19–0.54] | 0.5690 | 5 | 4 | 0.32 | [0.15–0.48] | 0.0000 | |
| Boromo | 25 | 8 | 7 | 1 | 0.28 | |0.10–0.45] | 0.7912 | 4 | 5 | 0.43 | [0.23–0.62] | 0.1201 | |
| Dédougou | 19 | 5 | 0 | 7 | 0.58 | [0.35–0.80] | 0.0004 | 7 | 0 | 0.29 | [0.08–0.49] | 0.0150 | |
| Koudougou | 26 | 9 | 2 | 8 | 0.47 | [0.27–0.66] | 0.0005 | 4 | 3 | 0.26 | [0.09–0.42] | 1 | |
| Nanoro | 5 | 1 | 0 | 3 | 0.75 | [0.37–1] | 0.1429 | 0 | 1 | 0.25 | [0–0.62] | 0.1429 | |
| Koupela | 24 | 7 | 1 | 6 | 0.46 | [0.26–0.65] | 0.0013 | 4 | 6 | 0.57 | [0.37–0.76] | 0.0003 | |
| Fada | 30 | 3 | 9 | 7 | 0.6 | [0.42–0.77] | 0.6254 | 5 | 6 | 0.44 | [0.26–0.61] | 0.0473 | |
| Kaya | 19 | 5 | 7 | 3 | 0.43 | [0.20–0.65] | 0.5785 | 3 | 1 | 0.16 | [0–0.32] | 0.0000 | |
| Ouahigouya | 30 | 10 | 3 | 7 | 0.42 | [0.24–0.59] | 0.0020 | 2 | 8 | 0.45 | [0.27–0.62] | 0.0632 | |
| Dori | 18 | 4 | 4 | 4 | 0.5 | [0.26–0.73] | 0.2300 | 1 | 5 | 0.55 | [0.32–0.77] | 0.0520 | |
N: number of mosquitoes
f(1014F): frequency of the kdr W resistant allele
f(1014S): frequency of the kdr E resistant allele
p(HW): probability of the exact test for goodness of fit to Hardy Weinberg equilibrium
-: not determined
Table 3. Allelic and genotypic frequencies at the ace-1 locus in An. gambiae s.l populations from 15 sites in Burkina Faso.
| Species | Sites | N | Genotypes | f(119S) | [95%Cl] | p(HW) | ||
|---|---|---|---|---|---|---|---|---|
| 119G 119G | 119G 119S | 119S 119S | ||||||
| An. arabiensis | Gaoua | 3 | 3 | 0 | 0 | 0 | - | - |
| Banfora | 0 | 0 | 0 | 0 | - | - | - | |
| Sindou | 5 | 5 | 5 | 0 | 0 | - | - | |
| Orodara | 1 | 1 | 1 | 0 | 0 | - | - | |
| Dioulassoba | 20 | 4 | 4 | 12 | 0.7 | [0.49–0.90] | 0.0264 | |
| Soumousso | 7 | 1 | 1 | 5 | 0.78 | [0.47–1] | 0.2308 | |
| Boromo | 15 | 5 | 9 | 1 | 0.36 | [0.11–0.60] | 0.9488 | |
| Dédougou | 14 | 4 | 6 | 4 | 0.5 | [0.23–0.76] | 0.0444 | |
| Koudougou | 12 | 5 | 0 | 7 | 0.58 | [0.30–0.85] | 0.0004 | |
| Nanoro | 3 | 2 | 0 | 1 | 0.33 | [0–0.86] | 0.2000 | |
| Koupela | 8 | 8 | 0 | 0 | 0 | - | - | |
| Fada | 13 | 4 | 8 | 1 | 0.38 | [0.11–0.64] | 0.9449 | |
| Kaya | 12 | 11 | 0 | 1 | 0.08 | [0–0.23] | 0.0435 | |
| Ouahigouya | 1 | 1 | 0 | 0 | 0 | - | - | |
| Dori | 14 | 14 | 0 | 0 | 0 | - | - | |
| An. coluzzii | Gaoua | 1 | 1 | 0 | 0 | 0 | - | - |
| Banfora | 6 | 6 | 0 | 0 | 0 | - | - | |
| Sindou | 6 | 6 | 0 | 0 | 0 | - | - | |
| Orodara | 4 | 4 | 0 | 0 | 0 | - | - | |
| Dioulassoba | 5 | 4 | 1 | 0 | 0.1 | [0–0.36] | - | |
| Soumousso | 3 | 3 | 0 | 0 | 0 | - | - | |
| Boromo | 0 | 0 | 0 | 0 | - | - | - | |
| Dédougou | 2 | 0 | 0 | 2 | 0.5 | [0–1] | 1 | |
| Koudougou | 5 | 2 | 3 | 0 | 0.3 | [0–0.70] | 1 | |
| Nanoro | 23 | 17 | 6 | 0 | 0.13 | [0–0.26] | 1 | |
| Koupela | 8 | 6 | 2 | 0 | 0.12 | [0–0.34] | 1 | |
| Fada | 27 | 27 | 0 | 0 | 0 | - | - | |
| Kaya | 5 | 5 | 0 | 0 | 0 | - | - | |
| Ouahigouya | 9 | 6 | 3 | 0 | 0.16 | [0–0.39] | 1 | |
| Dori | 5 | 5 | 0 | 0 | 0 | - | - | |
| An. gambiae | Gaoua | 36 | 22 | 11 | 3 | 0.23 | [0.09–0.36] | 0.2811 |
| Banfora | 24 | 20 | 4 | 0 | 0.08 | [0–0.18] | 1 | |
| Sindou | 24 | 21 | 3 | 0 | 0.06 | [0–0.15] | 1 | |
| Orodara | 23 | 22 | 0 | 1 | 0.04 | [0–0.12] | 0.0222 | |
| Dioulassoba | 4 | 4 | 0 | 0 | 0 | - | - | |
| Soumousso | 20 | 18 | 0 | 2 | 0.1 | [0–0.23] | 0.0021 | |
| Boromo | 15 | 9 | 4 | 2 | 0.26 | [0.03–0.48] | 0.2260 | |
| Dédougou | 12 | 8 | 4 | 0 | 0.16 | [0–0.36] | 1 | |
| Koudougou | 18 | 14 | 1 | 3 | 0.19 | [0–0.37] | 0.0029- | |
| Nanoro | 4 | 3 | 1 | 0 | 0.12 | [0–0.43] | - | |
| Koupela | 12 | 12 | 0 | 0 | 0 | - | - | |
| Fada | 19 | 18 | 0 | 1 | 0.05 | [0–14] | 0.0270 | |
| Kaya | 15 | 11 | 4 | 0 | 0.13 | [0–0.30] | 1 | |
| Ouahigouya | 19 | 14 | 2 | 3 | 0.21 | [0.02–0.39] | 0.0096 | |
| Dori | 11 | 10 | 0 | 1 | 0.09 | [0–0.25] | 0.0476 | |
N: number of mosquitoes
f(119S): frequency of the 119S resistant ace.1 allele
p(HW): probability of the exact test for goodness of fit to Hardy Weinberg equilibrium
-: not determined
Reference
- 1. Dabiré RK, Namountougou M, Diabaté A, Soma DD, Bado J, Toé HK, et al. (2014) Distribution and Frequency of kdr Mutations within Anopheles gambiae s.l. Populations and First Report of the Ace.1G119S Mutation in Anopheles arabiensis from Burkina Faso (West Africa). PLoS ONE 9(7): e101484 doi:10.1371/journal.pone.0101484 [DOI] [PMC free article] [PubMed] [Google Scholar]
