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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Neuroscience. 2015 Sep 14;310:322–341. doi: 10.1016/j.neuroscience.2015.09.006

Figure 1. Experimental schedule.

Figure 1

Female mice were singly housed with access to a running wheel starting at noon at age P36. After 5 days of acclimation to the wheel (1st Baseline), their food access was restricted to two hours per day (from 7 - 9 PM) for 3 days (1st ABA). At the end of the 1st ABA, they were housed in fresh cages with ad libitum food access and no running wheel. Following 7 days of recovery, access to a running wheel was restored. Following 4 days of wheel access with ad libitum food access (2nd Baseline), the second induction of ABA (2nd ABA) started, on P55. Mice were divided into two groups on P56, i.e. the second day of food restriction or FR2. The two groups were counterbalanced in terms of running activity on FR1. One group received P4 in oil and the other received only oil. All mice were perfused at noon on P59, at the end of the 2nd ABA, after four days of food restriction.