Fig. 5.

The tumorigenicity of the GC lines. (A–D) Kaplan–Meier graphs illustrating the survival of NOD-SCID mice injected intracranially with GC lines of the (A) Proneural (PN), (B) Neural (NL), (C) Classical (CL) and (D) Mesenchymal (MS) subtypes. (E) Summary of tumor formation and staining for STEM121 (human-specific). (F–G) H&E staining and immunostaining for STEM121 in the brains of mice injected with the U3024MG (F) or U3034MG (G) Mesenchymal GC lines. Black scale bar = 500 μm, white scale bar = 50 μm. (H) Kaplan–Meier graph comparing survival of NOD-SCID mice injected with GC lines of different subtypes. (I) H&E stainings of secondary tumors in NOD-SCID mice (left column) and the human tumor from which the cell line was derived (right column). Scale bar = 50 μm. See also Fig. S3.