Table 2.

Features of proMMP-7 binding to bicelles in the absence and presence of cholesterol 3-sulfate^*.

Property	Zwitterionic DMPC/D6PC	Anionic CSa+DMPC/D6PC
Tc of rotational diffusion	14.4 ns	20.9 ns
Vertical displacement†	39.6 ± 0.5 Å	28.4 ± 0.4 Å
Pitch (β)‡	62.1 ± 2.7°	7.5 ± 1.8°
Roll (γ)§	12.7 ± 3.8°	4.6 ± 1.2°
Buried surface area (BSA)	670 ± 70 Å2	2395 ± 116 Å2
Polar BSA∥	448 ± 45 Å2	1485 ± 99 Å2
Hydrophobic BSA	223 ± 39 Å2	910 ± 47 Å2
Convergent hydrophobic BSA∥	70 ± 23 Å2	745 ± 42 Å2

^*at ~3 per leaflet per bicelle on average. Uncertainties are SD.

^†measured between the centers of mass of the bilayer and catalytic domain

^‡This nautical angle is the Euler angle β between the membrane normal and the principal (longitudinal) axis of the zymogen (red in Fig. 2A,B).

^§This nautical angle is the Euler angle γ, defined as the rotation about the longitudinal axis, measured between the lateral axis along the active site helix and the bilayer surface.

^∥A detailed list of consistent, apparent contacts is provided in Table S1.