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. 1992 Feb;47(2):88–92. doi: 10.1136/thx.47.2.88

Sputum sol phase proteins and elastase activity in patients with clinically stable bronchiectasis.

P Lloberes 1, E Montserrat 1, J M Montserrat 1, C Picado 1
PMCID: PMC463577  PMID: 1372451

Abstract

BACKGROUND: Inflammatory and proteolytic activity occurs in sputum from patients with stable purulent bronchiectasis and has been proposed as the main pathogenetic mechanism of the disease. This study was designed to define further the role of inflammation and proteolysis in bronchiectasis. METHODS: Neutrophil elastase activity, sputum concentrations of the serum derived inhibitors alpha 1 antiproteinase and alpha 2 macroglobulin, and the sputum to serum ratios of albumin and C reactive protein concentration were measured in 26 patients with bronchiectasis. RESULTS: Free elastase activity was found in 15 sputum samples. A trend to higher proteolytic and inflammatory activity was found between mucoid and purulent sputum samples, suggesting that inflammatory and proteolytic activities are related to the macroscopic degree of purulence. Purulent sputum had a high sputum to serum ratio of C reactive protein, suggesting local production or active transport of this protein into bronchial secretions. C reactive protein was more sensitive than albumin in detecting a higher degree of inflammation in elastase positive samples. CONCLUSION: The finding of greater concentrations of alpha 2 macroglobulin in purulent and elastase positive samples than in mucopurulent, mucoid and elastase negative sputum samples suggests that this inhibitor may have a role in the proteolysis-antiproteolysis balance in bronchial secretions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adham N. F., Song M. K., Eng B. F. Hyper-alpha-2-macroglobulinemia in narcotic addicts. Ann Intern Med. 1978 Jun;88(6):793–795. doi: 10.7326/0003-4819-88-6-793. [DOI] [PubMed] [Google Scholar]
  2. Brown G. M., Donaldson K. Degradation of connective tissue components by lung derived leucocytes in vitro: role of proteases and oxidants. Thorax. 1988 Feb;43(2):132–139. doi: 10.1136/thx.43.2.132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burnett D., Stockley R. A. Serum and sputum alpha 2 macroglobulin in patients with chronic obstructive airways disease. Thorax. 1981 Jul;36(7):512–516. doi: 10.1136/thx.36.7.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burnett D., Stockley R. A. Serum and sputum alpha 2 macroglobulin in patients with chronic obstructive airways disease. Thorax. 1981 Jul;36(7):512–516. doi: 10.1136/thx.36.7.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cochrane G. M. Chronic bronchial sepsis and progressive lung damage. Br Med J (Clin Res Ed) 1985 Apr 6;290(6474):1026–1027. doi: 10.1136/bmj.290.6474.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jackson A. H., Hill S. L., Afford S. C., Stockley R. A. Sputum sol-phase proteins and elastase activity in patients with cystic fibrosis. Eur J Respir Dis. 1984 Feb;65(2):114–124. [PubMed] [Google Scholar]
  7. Lapa e Silva J. R., Guerreiro D., Noble B., Poulter L. W., Cole P. J. Immunopathology of experimental bronchiectasis. Am J Respir Cell Mol Biol. 1989 Oct;1(4):297–304. doi: 10.1165/ajrcmb/1.4.297. [DOI] [PubMed] [Google Scholar]
  8. Stockley R. A., Burnett D. Serum derived protease inhibitors and leucocyte elastase in sputum and the effect of infection. Bull Eur Physiopathol Respir. 1980;16 (Suppl):261–272. doi: 10.1016/b978-0-08-027379-2.50027-2. [DOI] [PubMed] [Google Scholar]
  9. Stockley R. A., Hill S. L., Morrison H. M., Starkie C. M. Elastolytic activity of sputum and its relation to purulence and to lung function in patients with bronchiectasis. Thorax. 1984 Jun;39(6):408–413. doi: 10.1136/thx.39.6.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stockley R. A., Mistry M., Bradwell A. R., Burnett D. A study of plasma proteins in the sol phase of sputum from patients with chronic bronchitis. Thorax. 1979 Dec;34(6):777–782. doi: 10.1136/thx.34.6.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stockley R. A. Proteolytic enzymes, their inhibitors and lung diseases. Clin Sci (Lond) 1983 Feb;64(2):119–126. doi: 10.1042/cs0640119. [DOI] [PubMed] [Google Scholar]
  12. Wilson R., Roberts D., Cole P. Effect of bacterial products on human ciliary function in vitro. Thorax. 1985 Feb;40(2):125–131. doi: 10.1136/thx.40.2.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Beer F. C., Nel A. E., Gie R. P., Donald P. R., Strachan A. F. Serum amyloid A protein and C-reactive protein levels in pulmonary tuberculosis: relationship to amyloidosis. Thorax. 1984 Mar;39(3):196–200. doi: 10.1136/thx.39.3.196. [DOI] [PMC free article] [PubMed] [Google Scholar]

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