There is an error in the third sentence of the “16S library preparation” subsection of the Materials and Methods. The first primer listed is incorrect. The correct sentence is: The V6 region was amplified with custom barcoded primers that consist of a region that anneals to the V6 region of interest, followed by a unique five basepair barcode, and the Illumina sequencing adapter [44]:
V6-L [5’- ACACTCTTTCCCTACACGACGCTCTTCCGATCTnnnnnCWACGCGARGAACCTTACC-3’]
V6-R [5’-CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTnnnnnACRACACGAGCTGACGAC-3’]
Reference
- 1. Gignoux-Wolfsohn SA, Vollmer SV (2015) Identification of Candidate Coral Pathogens on White Band Disease-Infected Staghorn Coral. PLoS ONE 10(8): e0134416 doi:10.1371/journal.pone.0134416 [DOI] [PMC free article] [PubMed] [Google Scholar]