Time-resolved fluorescence of IR-dyes (5 µmol/L) and IR-dyes/β-NaYF4:20%Yb,2%Er NPs (5 µmol/L : 0.1 µmol/L) were respectively excited at 780 nm, 800 nm, 820 nm, 840 nm and detected at 820 nm, 835 nm, 850 nm, 865 nm by 150 fs pulsed 300 micron slit width laser. The decrease of fluorescence lifetime of IR-dyes upon of NPs indicated energy transfer from IR-dye to nanoparticles.