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. 2015 Jun 2;6(23):19759–19779. doi: 10.18632/oncotarget.4331

Figure 3. TUG1 regulates BTB permeability and the expression of tight junction related proteins in GEC.

Figure 3

A. In vitro BTB models were established using EC with stably knockdown of TUG1, relative expression levels of TUG1 were detected by quantitative real-time PCR. B. The Transendothelial electric resistance (TEER) values of BTB were detected after knockdown of TUG1 in GEC. C. Permeability assays were performed by HRP flux test. D. Western blot analysis of TJ-related proteins ZO-1, occludin, and claudin-5 in GEC. The integrated light density values (IDVs) of protein expression levels of TJ-related proteins are shown. Data represent mean ± SD (n = 5, each). *P < 0.05 vs. TUG1 (−) NC group. E. Immunofluorescence localization of ZO-1, occludin and claudin-5 in GEC. Nuclei are labeled with DAPI. Images are representative of five independent experiments. Original magnification: 100x. Scale bar = 5 um.