High quality draft genome sequence of the moderately halophilic bacterium Pontibacillus yanchengensis Y32^T and comparison among Pontibacillus genomes

Abstract

Pontibacillus yanchengensis Y32^T is an aerobic, motile, Gram-positive, endospore-forming, and moderately halophilic bacterium isolated from a salt field. In this study, we describe the features of P. yanchengensis strain Y32^T together with a comparison with other four Pontibacillus genomes. The 4,281,464 bp high-quality-draft genome of strain Y32^T is arranged into 153 contigs containing 3,965 protein-coding genes and 77 RNA encoding genes. The genome of strain Y32^T possesses many genes related to its halophilic character, flagellar assembly and chemotaxis to support its survival in a salt-rich environment.

Electronic supplementary material

The online version of this article (doi:10.1186/s40793-015-0085-y) contains supplementary material, which is available to authorized users.

Introduction

Pontibacillus yanchengensis Y32^T (= CGMCC 1.10680^T = CCTCC AB209311^T = NRRL B-59408^T) was isolated from a salt field in Yancheng, China [1], and affiliated to the family Bacillaceae, order Bacillales, phylum Firmicutes [2, 3]. The genus Pontibacillus means “Bacillus pertaining to the sea” and was first identified by Lim et al. in 2005 [4]. To date, the genus contains six species, including Pontibacillus yanchengensis [1], Pontibacillus chungwhensis [4], Pontibacillus marinus [5], Pontibacillus halophilus [6], Pontibacillus litoralis [7], and Pontibacillus salicampi [8], which are isolated from a salt field, a solar saltern, a solar saltern, a sea urchin, a sea anemone, and a saltern soil, respectively.

The Pontibacillus members are characterized as moderately halophilic, Gram-positive, aerobic, endospore-forming and rod-shaped bacteria. They are motile by peritrichous flagella and their DNA has a low G + C content. They are able to survive in salt-rich environments and grow optimally at 5-20 % NaCl (w/v) [9]. To adapt to saline environments, halophilic microorganisms have developed various biochemical strategies to maintain cell function, such as induction of Na⁺/H⁺ antiporter systems and the production of compatible solutes. The compatible solutes are gaining increasing interest since they can be used as stabilizers, salt antagonists, or stress-protective agents [10–13]. In addition, a Pontibacillus strain could produce biosurfacants which is useful in degradation of paraffinic mixture or saline organic contamination [11].

In this study, we sequenced five Pontibacillus type strains, including P. yanchengensis Y32^T,P. chungwhensis BH030062^T, P. marinus BH030004^T, P. halophilus JSM076056^T and P. litoralis JSM072002^T(The GenBank accession summary of the strains is shown in Additional file 2). Here we present the draft genome sequence of P. yanchengensis Y32^T and compare it to the genomes of four other type strains. To the best of our knowledge, this is the first description of the Pontibacillus genome.

Organism information

Classification and features

P. yanchengensis Y32^T was isolated from a salt field in Yancheng prefecture, on the east Yellow Sea in China. A taxonomic analysis was conducted based on the 16S rRNA gene sequence. The representative 16S rRNA gene sequences of the most closely related strains were downloaded from NCBI and multi-aligned by CLUSTAL W [14]. Phylogenetic consensus trees were constructed based on the aligned gene sequences using the neighbor-joining method with 1,000 bootstraps by using MEGA 6.0 [15]. The phylogenetic tree based on the 16S rRNA gene sequences indicated that strain Y32^T was clustered within a branch containing other species in the genus Pontibacillus (Fig. 1a).

Fig. 1.

Phylogenetic analysis. a The 16S rRNA gene-based phylogenetic tree showing the position of P. yanchengensis Y32^T. b The NJ phylogenetic tree of P. yanchengensis Y32^T relative to 16 genome-sequenced strains from the Bacillaceae family was built based on the core protein sequences. All genome FASTA files were downloaded from NCBI except for the Pontibacillus genus. A total of 602 conserved proteins were identified using the cluster algorithm tool OrthoMCL [16, 17]. The phylogenetic trees were constructed using the neighbor-joining method by MEGA 6.0 software [15] with a bootstrap value of 1,000

Seventeen related strains of Bacillaceae [2] with complete genome sequences were chosen for further phylogenetic analysis, including the four draft-genome sequences of Pontibacillus that were sequenced by us. In total, 602 core protein sequences were extracted using the cluster algorithm tool OrthoMCL [16, 17] with default parameters. The neighbor-joining (NJ) phylogenetic tree showed that the five Pontibacillus species clustered into the same branch (Fig. 1b), which was in accordance with the 16S rRNA gene-based phylogeny (Fig. 1a).

P. yanchengensis Y32^T is Gram-positive, rod-shaped (0.5–0.9 × 1.9–2.5 μm), motile with flagella (Fig. 2) and endospore-forming. It can grow on Bacto marine broth 2216 (Difco) agar medium containing 3–20 % (w/v) NaCl and does not grow in the absence of NaCl [1]. The optimal growth temperature for Y32^T is 35–40 °C (Table 1). The strain is oxidase- and catalase-positive and negative for the production of H₂S or indole. It has been reported to reduce nitrate to nitrite [1]. P. yanchengensis Y32^T can use a few kinds of sole carbon sources, including D-glucose, D-fructose, D-mannitol, D-maltose and D-trehalose [1]. Compared to the other Pontibacillus genus type strains, only P. yanchengensis Y32^T can utilize D-mannitol as sole carbon source [1]. KEGG pathway analysis of the five Pontibacillus genomes (see below) revealed that only strain Y32^T had the key enzyme mannitol-1-phosphate 5-dehydrogenase (gene ID: N782_14920) which could potentially catalyze D-mannitol 1-phosphate to D-fructose 6-phosphate. This result was consistent with the phenotype. As one of the most abundant polyols in nature, mannitol metabolism provides an important physiologic contribution in microbial stress responses [18].

Fig. 2.

A transmission micrograph of P. yanchengensis Y32^T. The scale bar represents 1 μm. Strain Y32^T was cultured aerobically on Bacto marine broth 2216 (Difco) agar plus 5 % NaCl at 37C° for 48 h

Table 1.

Classification and general features of P. yanchengensis Y32^T according to the MIGS recommendations [26]

MIGS ID	Property	Term	Evidence code
	Current classification	Domain Bacteria	TAS [27]
		Phylum Firmicutes	TAS [28]
		Class Bacilli	TAS [29, 30]
		Order Bacillales	TAS [2, 3]
		Family Bacillaceae	TAS [2, 3]
		Genus Pontibacillus	TAS [4]
		Species Pontibacillus yanchengensis	TAS [1]
		Type strain Y32T	TAS [1]
	Gram stain	Positive	TAS [1]
	Cell shape	Rod-shaped	TAS [1]
	Motility	Motile	TAS [1]
	Sporulation	Sporulating	TAS [1]
	Temperature range	15–45 °C	TAS [1]
	Optimum temperature	35–40 °C	TAS [1]
	Salinity	3–20 % (w/v)	TAS [1]
	Optimum salinity	6–8 % (w/v)	TAS [1]
	pH range	6–9.5	TAS [1]
	Optimum pH	7–8	TAS [1]
MIGS-22	Oxygen	aerobic	TAS [1]
MIGS-15	Biotic relationship	Free-living	NAS
MIGS-6	Habitat	Salt-field	TAS [1]
MIGS-14	Pathogenicity	Non-pathogenic	NAS
MIGS-4	Geographic location	Yancheng, China	TAS [1]
MIGS-4.1	Latitude	N32°23'	TAS [1]
MIGS-4.2	Longitude	E120°0'	TAS [1]
MIGS-4.3	Depth	5–15 cm	TAS [1]
MIGS-4.4	Altitude	Not reported
MIGS-5	Sample collection time	2011	TAS [1]

Evidence codes - TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [31]

Chemotaxonomic data

When grown on Bacto marine broth 2216 (Difco) agar medium plus 3 % (w/v) NaCl, P. yanchengensis Y32^T contained anteiso-C_15:0, iso-C_15:0, and iso-C_14:0 as the major fatty acids and menaquinone (MK-7) as the predominant respiratory quinone. The cell wall peptidoglycan type was meso-diaminopimelic [1]. The classification and general features of P. yanchengensis Y32^T are shown in Table 1.

Genome sequencing information

Genome project history

P. yanchengensis Y32^T was selected for sequencing on the basis of its taxonomic representativeness, halophilic features and potential industrial applications. Genome sequencing was performed by Majorbio Bio-pharm Technology Co., Ltd., Shanghai, China. The draft genome sequence was deposited in NCBI with contigs larger than 200 bp. The GenBank accession number is AVBF00000000. A summary of the genome sequencing project information is shown in Table 2.

Table 2.

Genome sequencing project information for P. yanchengensis Y32^T

MIGS ID	Property	Term
MIGS-31	Finishing quality	High-quality draft
MIGS-29	Libraries used	Illumina Paired-End library (300 bp insert size)
MIGS-29	Sequencing platform	Illumina Hiseq2000
MIGS-31.2	Sequencing coverage	186.5 x
MIGS-30	Assemblers	SOAP denovo v1.05
	Gene calling method	GeneMarkS+
	GenBank date of release	November 6, 2014
	GenBank ID	AVBF00000000
	Locus Tag	N782
MIGS-32	BIOPROJECT	PRJNA214569
	BioSample	SAMN02650962
MIGS-13	Source material identifier	Y32T
	Project relevance	Genome comparison

Growth conditions and DNA isolation

P. yanchengensis Y32^T was grown aerobically in 50 mL Bacto marine broth 2216 (Difco) plus 5 % NaCl (w/v) at 37 °C for 2 d with 150 rpm shaking. Cells were harvested by centrifugation and a pellet with an approximate wet weight of 20 mg was obtained. The genomic DNA was extracted using the QIAamp DNA kit according to the manufacturer’s instructions (Qiagen, Germany). The quality and quantity of total DNA was determined using a NanoDrop Spectrophotometer 2000. Five micrograms of genomic DNA was sent to Majorbio (Shanghai, China) for sequencing on a Hiseq2000 (Illumina, CA) sequencer.

Genome sequencing and assembly

The Illumina Hiseq2000 technology of Paired-End (PE) library with an average insert size of 300 bp was used to determine the sequence of P. yanchengensis Y32^T. A total of 4,083,912 × 2 high quality reads totaling 824,950,224 bp of data with an average coverage of 186.5 x was generated. Raw reads were filtered using a FastQC toolkit followed by assembly with SOAP denovo v1.05 and optimizing through local gap filling and base correction with Gap Closer.

Genome annotation

The draft genome sequence was deposited at NCBI and was annotated through the Prokaryotic Genome Annotation Pipeline, which combined the Best-Placed reference protein set and the gene caller GeneMarkS+. The WebMGA server was used to identify the Clusters of Ortholog Groups [19]. Transmembrane helices and signal peptides were predicted by the online bioinformatic tools TMHMM 2.0 [20, 21] and SignalP 4.1 [22], respectively.

Genome properties

The final whole genome of P. yanchengensis Y32^T was 4,283,464 bp long, distributed in 153 contigs, and had an average GC content of 39.11 %. Of the total 4,080 predicted genes, 3,965 were protein-coding genes (CDSs), and 77 were RNA genes. A total of 2,615 CDSs (65.95 %) were assigned putative functions, and the remaining proteins were annotated as hypothetical proteins. The genome properties and statistics are summarized in Table 3. The distribution of genes into COGs functional categories is shown in Table 4.

Table 3.

Genome statistics for P. yanchengensis Y32^T

Attribute	Value	% of Totala
Genome size (bp)	4,281,464	100.00
DNA coding region (bp)	3.472,267	81.10
DNA G + C content (bp)	1,674,480	39.11
Number of contigs	153	-
Contig N50 (bp)	55350	-
Total genes	4080	100.00
Protein-coding genes	3965	97.18
RNA genes	77	1.89
Pseudo genes	38	0.93
Frame shifted genes	12	-
Genes with function prediction	2615	65.95
Genes assigned to COGs	2972	74.95
Genes with Pfam domains	3135	79.07
Genes with signal peptides	242	6.10
Genes with transmembrane helices	1176	29.66
CRISPR repeats	0	-

^aThe total is based on either the size of the genome in base pairs or the total number of protein coding genes in the annotated genome

Table 4.

Number of protein-coding genes associated with the 25 general COG functional categories in the P. yanchengensis Y32^T genome

COG class	Count	% agea	COG description
J	171	4.31	Translation, ribosomal structure and biogenesis
A	0	0.00	RNA processing and modification
K	258	6.51	Transcription
L	148	3.73	Replication, recombination and repair
B	2	0.05	Chromatin structure and dynamics
D	36	0.91	Cell cycle control, cell division, chromosome partitioning
Y	0	0.00	Nuclear structure
V	54	1.36	Defense mechanisms
T	222	5.60	Signal transduction mechanisms
M	173	4.36	Cell wall/membrane/envelope biogenesis
N	71	1.79	Cell motility
Z	0	0.00	Cytoskeleton
W	0	0.00	Extracellular structures
U	58	1.46	Intracellular trafficking, secretion, and vesicular transport
O	119	3.00	Posttranslational modification, protein turnover, chaperones
C	199	5.02	Energy production and conversion
G	253	6.38	Carbohydrate transport and metabolism
E	295	7.44	Amino acid transport and metabolism
F	94	2.37	Nucleotide transport and metabolism
H	132	3.33	Coenzyme transport and metabolism
I	129	3.25	Lipid transport and metabolism
P	186	4.69	Inorganic ion transport and metabolism
Q	71	1.79	Secondary metabolites biosynthesis, transport and catabolism
R	433	10.92	General function prediction only
S	353	8.9	Function unknown
-	508	12.81	Not in COGs

^aThe percentage is based on the total number of protein-coding genes in the annotated genome

Insights from the genome sequence

In this study, we compared the genome sequence of P. yanchengensis Y32^T with the genomes of P. chungwhensis BH030062^T, P. halophilus JSM076056^T, P. marinus BH030004^T and P. litoralis JSM072002^T. The general features of the five genomic sequences are summarized in Table 5. The results of the core genome analysis suggested that the five Pontibacillus species share 2,160 core genes, and P. yanchengensis Y32^T possesses 1,651 unique genes (Fig. 3a). Among the 1,651 unique genes for strain Y32^T, 1,154 unique genes were classified into 20 COG functional categories, which mainly belonged to the general function prediction group, the carbohydrate transport, the metabolism group and the function unknown group. The remaining 590 unique genes were not classified into any COG categories (Additional file 1: Table S1). The CG View Comparison Tool [23] was used to draw a comparison graphical circular map of the five Pontibacillus strains (Fig. 3b).

Table 5.

General features of the five Pontibacillus genome sequences

Organism	Source	Genome size (bp)	G + C%	Contigs	Contigs N50 (bp)	Genes	CDS	RNA	GenBank No.	CRISPR
P. yanchengensis Y32T	Salt field	4,283,159	39.11	153	55,350	4,080	3,965	77	AVBF00000000	0
P. chungwhensis BH030062T	Solar saltern	3,873,758	40.76	40	225,560	3,801	3,685	62	AVBG00000000	0
P. marinus BH030004T	Solar saltern	4,275,582	38.48	186	45,534	4,329	4,253	52	AVPF00000000	0
P. halophilus JSM076056T	Sea urchin	3,694,752	42.85	68	190,345	3,653	3,560	58	AVPE00000000	0
P. litoralis JSM072002T	Sea anemone	3,205,664	38.18	97	112,260	3,282	3,202	49	AVPG00000000	3

Fig. 3.

Comparative genomic analysis of the genus Pontibacillus. a The flower plot shows the numbers of species-specific genes found in each genome of each species (in the petals) and the core orthologous gene number (in the center) of Pontibacillus. b Comparison map of strain P. yanchengensis Y32^T and the other four sequenced Pontibacillus strains. From outside to inside: rings 1, 4 show protein-coding genes colored by COG categories on the forward/reverse strand, respectively; rings 2, 3 represent genes on the forward/reverse strand, respectively; rings 5, 6, 7, 8 denote the CDS vs CDS BLAST results of P. marinus BH030004^T, P. chungwhensis BH030062^T, P. halophilus JSM076056^T, and P. litoralis JSM072002^T, respectively; ring 9 shows the GC skew

All the Pontibacillus species were isolated from salty environments. They were characterized as moderately halophilic and cannot grow in the absence of NaCl. As moderate halophiles, effective establishment of ionic and osmotic equilibrium was important for survival in a saline environment. The genome comparison analysis showed that the five Pontibacillus strains possessed genes encoding cation/proton antiporter (e.g., Na⁺/H⁺ antiporter, Na⁺/Ca²⁺ antiporter), which played a role in tolerance to high concentrations of Na⁺, K⁺, Li⁺ and/or alkali (Additional file 1: Table S2). Numerous studies showed that Na⁺/H⁺ antiporters play important roles in the pH and Na⁺ homeostasis of cells [24, 25]. Meanwhile, the prediction of the membrane helices of the P. yanchengensis Y32^T genome suggested that nearly 30% of the genes had transmembrane helix structures (Table 3), which may be involved in ion transport.

Other than ion transport, the synthesis of compatible solutes (e.g., betaine, ectoine, amino acids) was beneficial for survival under extreme osmotic stress. Many compatible solute synthesis-related genes were identified in the genomes of the five Pontibacillus species (Additional file 1: Table S2). The Kyoto Encyclopedia of Genes and Genomes was used to reconstruct the glycine, serine and threonine metabolic pathways (Fig. 4). The metabolic pathways suggested that the five Pontibacillus strains could synthesize glycine as the main compatible solute. In addition, P. yanchengensis Y32^T, P. chungwhensis BH030062^T and P. marinus BH030004^T could synthesize betaine through the precursor choline. P. marinus BH030004^T also possessed the pathway of ectoine synthesis. These results indicated that the five Pontibacillus species use different strategies to cope with osmotic stress.

Fig. 4.

The glycine, serine and threonine metabolic pathways of the five Pontibacillus strains (including P. yanchengensis Y32^T, P. marinus BH030004^T, P. chungwhensis BH030062^T, P. halophilus JSM076056^T, and P. litoralis JSM072002^T) reconstructed by KEGG. The green box represents the enzyme shared by all five strains to synthesize glycine. The blue boxes denote the enzymes involved in betain synthesis, which were found in P. yanchengensis Y32^T, P. chungwhensis BH030062^T and P. marinus BH030004^T. The pathway with pink boxes is found only by P. marinus BH030004^T and is related to ectoine synthesis

Many flagella-related genes were identified in the genomes of the five Pontibacillus species. Reconstruction of a multi-organism KEGG map suggested that the five Pontibacillus strains had intact chemotaxis systems (Fig. 5a) and flagella assembly-related genes (flg, fli and flh) (Fig. 5b). The moderately halophilic Pontibacillus strains were unable to grow with NaCl as the sole salt unless artificial seawater was added [1, 4–8]. Flagella and chemotaxis may play important roles in response to environmental salts.

Fig. 5.

Reconstructed bacterial chemotaxis (a) and flagellar assembly (b) KEGG map of the five Pontibacillus strains. Green boxes represent the chemotaxis- and flagellar-related protein-coding genes identified in all five Pontibacillus genomes (P. yanchengensis Y32^T, P. marinus BH030004^T, P. chungwhensis BH030062^T, P. halophilus JSM076056^T, and P. litoralis JSM072002^T)

Conclusions

This study provided genomic information for P. yanchengensis strain Y32^T and the comparison of five Pontibacillus genomes. Strain Y32^T has functional genes encoding cation/proton antiporters and proteins for biosynthesis of compatible solutes such as glycine and ectoine. Compatible solutes could be of use in the cosmetic and food industries [13]. The comparative genomic analysis suggested that the five Pontibacillus strains possess different synthetic pathways for compatible solutes which provided diverse applications of the strains.

Additional files

Additional file 1: Table S1. ^{(15.6KB, docx)}

COG functional categories of the 1651 genes unique to P.yanchengensis Y32^T. Table S2 Species distribution analysis of osmotic stress related gene families. (DOCX 15 kb)

Additional file 2: ^{(33KB, doc)}

GenBank Accession Summary, Strain ID Summary, Reference Search Summary. (DOC 33 kb)