Figure 3. Feedback from MBON-α1 is required for appetitive LTM formation.
(A) Diagram of the experiment. (B) Expression pattern of MB299B-GAL4. (C) Blockade of PAM-α1 does not impair STM significantly. 5-min appetitive memory was measured (n = 10, 10, 12). c: conditioning, t: test. (D) Blockade of PAM-α1 during conditioning impairs LTM. 24-hr appetitive LTM was measured (n = 9, 12, 12). (E) Diagram of experiment. (F) Expression pattern of MB310C-GAL4. (G) Blockade of MBON-α1 does not impair STM significantly (n = 6, 7, 10, 6, 10). (H) Blockade of MBON-α1 during conditioning impairs LTM. MB323B-GAL4 is a second driver line that expresses in MBON-α1 (see figure supplement; n = 23, 24, 13, 14, 24). Bar graphs are mean ± s.e.m. *: p < 0.05, ***: p < 0.001, n.s.: p > 0.05. Scale bars, 20 µm.
Figure 3—figure supplement 1. (A) Expression pattern of MB323B-GAL4.
Scale bar, 20 µm. (B) The LTM performance of MB310C/UAS-shi and MB323B/UAS-shi flies at the permissive temperature is not affected (n = 17, 16, 8, 9, 11). n.s.: p > 0.05.