Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units.

VOLUME 288 (2013) PAGES 25976–25985

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The legend for Fig. 2 should be revised as follows.

FIGURE 2. 6X11 and 6X12 are serologically distinct from other members in serogroup 6. Flow cytometry histograms of various pneumococcal strains (indicated at the left of each row) that were stained with different mAbs (indicated at the bottom of each column) are shown. The shaded area in each panel represents non-specific staining by the secondary antibodies and was obtained by simultaneously staining TIGR6A with both anti-IgG and anti-IgM antibodies in the absence of monoclonal antibodies. In unpublished experiments, all isogenic serogroup 6 strains had low staining by secondary antibodies (mean fluorescence index <5).

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“Fig. 3A” and “Fig. 3B” should be corrected to “Fig. 3.”

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In Fig. 4, panels A and B were switched. The corrected figure and legend are shown below.

FIGURE 4.

Overlay of ¹H-¹³C HMQC spectra of serotypes 6A (black) and 6X12 (red) (A) and 6B (black) and 6X11 (red) (B). For 6X12 and 6X11, new glucose (Glc′) signals appear indicating their PSs are a mixture of two different repeating units (RU). A, the chemical shifts (ppm) of the labeled Gal and Glc′ peaks of 6X12 and 6A overlap at 5.60 ppm, indicating ∼75% of 6X12 RUs are identical to 6A, whereas 25% are 6C-like because they contain glucose′. B, anomeric signals of Gal and Glc′ of 6X11 and 6B overlap at 5.55–5.60 ppm, indicating ∼40% of 6X11 RUs are identical to 6B, whereas 60% are 6D-like because they contain Glc′. C, proposed structural models of 6X12 and 6X11 PS.