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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 May 1;90(9):4176–4180. doi: 10.1073/pnas.90.9.4176

Controlled release of polypeptides from polyanhydrides.

E Ron 1, T Turek 1, E Mathiowitz 1, M Chasin 1, M Hageman 1, R Langer 1
PMCID: PMC46469  PMID: 8483931

Abstract

The effects of both polymer hydrophobicity and addition of stabilizers on the release and integrity of polymer-encapsulated proteins were studied. By using very hydrophobic poly[1,3-bis(p-carboxyhydroxy)hexane anhydride] with sucrose as an excipient, both recombinant bovine somatotropin and zinc insulin were released intact over 3 weeks. The released proteins appeared to maintain their integrity as judged by acidic reverse-phase HPLC, size-exclusion HPLC, radioimmunoassay, and conformation-sensitive immunoassays. Our results also suggest how polymer hydrophobicity can be used to enhance protein stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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