Erratum for Uda et al., Establishment of an In Vitro d-Cycloserine-Synthesizing System by Using O-Ureido-l-Serine Synthase and d-Cycloserine Synthetase Found in the Biosynthetic Pathway

ERRATUM

Volume 57, no. 6, p. 2603–2612, 2013. Page 2609, right column, first full paragraph, line 6: “2.1 × 10⁵ ± 0.3 × 10⁵ M^–1 s^–1” should read “2.1 × 10² ± 0.3 × 10² M^–1 s^–1.”

Page 2610: The values in Table 2 should appear as shown below.

TABLE 2.

Kinetic parameters of DcsG

Substrate	Mean Km (mM)a ± SD	Mean kcat (s−1)a ± SD	Mean kcat/Km (M−1 s−1) ± SD	Mean Kmapp for ATP (μM)b ± SD
d-OUS	10 ± 1	2.2 ± 0.2	210 ± 30	73 ± 8
β-Aminooxy-d-alanine	NDc	NDc	14 ± 1	37 ± 3
d-2,4-Diaminobutyrate	NDc	NDc	2.1 ± 0.1	45 ± 7
d-Ornithine	NDc	NDc	0.67 ± 0.04	45 ± 2
d-Homocysteine	19 ± 3	0.86 ± 0.06	45 ± 9	23 ± 6
d-Homoserine	NDc	NDc	6.1 ± 0.3	54 ± 10

^aK_m and k_cat were determined in the presence of 5 mM ATP.

^bK_m^app for ATP was determined in the presence of 50 mM d-amino acid.

^cSince K_m was too high, K_m and k_cat were not determined (ND).